LOCUS       sequence-236192-        6659 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression Brg1 amino acids 1-381 in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6659)
  AUTHORS   Zhou J, Zhang M, Fang H, El-Mounayri O, Rodenberg JM, Imbalzano AN, 
            Herring BP
  TITLE     The SWI/SNF chromatin remodeling complex regulates myocardin-induced
            smooth muscle-specific gene expression.
  JOURNAL   Arterioscler Thromb Vasc Biol. 2009 Jun;29(6):921-8. doi: 
            10.1161/ATVBAHA.109.187229. Epub 2009 Apr 2.
  PUBMED    19342595
REFERENCE   2  (bases 1 to 6659)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1161/ATVBAHA.109.187229"; journalName: "Arterioscler Thromb Vasc
            Biol"; date: "2009-06"; volume: "29"; issue: "6"; pages: "921-8"
COMMENT     Sequence Label: Brg1 Del1 pET28a
FEATURES             Location/Qualifiers
     source          1..6659
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..25
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             40..62
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             81..98
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             108..125
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             129..161
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             1505..1522
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(1575..1593)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1589..1636
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      1673..2128
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1760..1779)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1970..1991
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(2220..3035)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(2220..3035)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2943..2962
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     3027..3046
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      3157..3745
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3646..3665
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3899..3916
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3931..4070
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3996..4015)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4156..4178
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(4172..4363)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(5172..6254)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     6216..6235
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(6255..6332)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     6538..6557
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     join(6641..6659,1)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6641..6659
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggaattgtga gcggataaca attcccctct agaaataatt ttgtttaact ttaagaagga
       61 gatataccat gggcagcagc catcatcatc atcatcacag cagcggcctg gtgccgcgcg
      121 gcagccatat ggctagcatg actggtggac agcaaatggg tcgcggatcc ggtggtggtg
      181 caaatcaaag aactgctcct cagtggatgt tgcctttact tctaggcctg tacggaagtg
      241 ttacttctgc tctaaaagct gctctagccc tcgacggcgc cggcggctcc tgcaggaggc
      301 cactgtctgc agctcccgtg aagatgtcca ctccagaccc acccctgggc ggaactcctc
      361 ggccaggtcc ttccccgggc cctggccctt cccctggagc catgctgggc cctagcccgg
      421 gtccctcgcc gggctccgcc cacagcatga tggggcccag cccagggccg ccctcagcag
      481 gacaccccat ccccacccag gggcctggag ggtaccctca ggacaacatg caccagatgc
      541 acaagcccat ggagtccatg catgagaagg gcatgtcgga cgacccgcgc tacaaccaga
      601 tgaaaggaat ggggatgcgg tcagggggcc atgctgggat ggggcccccg cccagcccca
      661 tggaccagca ctcccaaggt tacccctcgc ccctgggtgg ctctgagcat gcctctagtc
      721 cagttccagc cagtggcccg tcttcggggc cccagatgtc ttccgggcca ggaggtgccc
      781 cgctggatgg tgctgacccc caggccttgg ggcagcagaa ccggggccca accccattta
      841 accagaacca gctgcaccag ctcagagctc agatcatggc ctacaagatg ctggccaggg
      901 ggcagcccct ccccgaccac ctgcagatgg cggtgcaggg caagcggccg atgcccggga
      961 tgcagcagca gatgccaacg ctacctccac cctcggtgtc cgcaacagga cccggccctg
     1021 gccctggccc tggccccggc ccgggtcccg gcccggcacc tccaaattac agcaggcctc
     1081 atggtatggg agggcccaac atgcctcccc caggaccctc gggcgtgccc cccgggatgc
     1141 caggccagcc tcctggaggg cctcccaagc cctggcctga aggacccatg gcgaatgctg
     1201 ctgcccccac gagcacccct cagaagctga ttcccccgca gccaacgggc cgcccttccc
     1261 ccgcgccccc tgccgtccca cccgccgcct cgcccgtgat gccaccgcag acccagtccc
     1321 ccgggcagcc ggcccagccc gcgcccatgg tgccactgca ccagaagcag agccgcatca
     1381 cccccatcca gaagccgcgg ggcctcgacc ctgtggagat cctgcaggag cgcgagtaca
     1441 ggctgcaggc tcgcatcgca caccgaattc gagctccgtc gacaagcttg cggccgcact
     1501 cgagcaccac caccaccacc actgagatcc ggctgctaac aaagcccgaa aggaagctga
     1561 gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt
     1621 cttgaggggt tttttgctga aaggaggaac tatatccgga ttggcgaatg ggacgcgccc
     1681 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     1741 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     1801 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     1861 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     1921 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     1981 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     2041 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     2101 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
     2161 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg aattaattct
     2221 tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata
     2281 ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat
     2341 aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct
     2401 attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact
     2461 gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag
     2521 ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc
     2581 gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa
     2641 tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat
     2701 tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca
     2761 tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt
     2821 agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac
     2881 aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca
     2941 ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc
     3001 ctagagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg
     3061 taagcagaca gttttattgt tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     3121 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     3181 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     3241 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     3301 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     3361 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     3421 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     3481 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     3541 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     3601 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     3661 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     3721 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     3781 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     3841 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     3901 cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct
     3961 gtgcggtatt tcacaccgca atggtgcact ctcagtacaa tctgctctga tgccgcatag
     4021 ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc
     4081 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
     4141 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
     4201 gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg
     4261 cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga
     4321 taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag
     4381 ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac
     4441 gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg
     4501 tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata
     4561 cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa
     4621 tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca
     4681 ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc
     4741 gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca
     4801 acgacaggag cacgatcatg cgcacccgtg gggccgccat gccggcgata atggcctgct
     4861 tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga
     4921 ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc
     4981 cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag
     5041 tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga
     5101 aggctctcaa gggcatcggt cgagatcccg gtgcctaatg agtgagctaa cttacattaa
     5161 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     5221 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgccagggt ggtttttctt
     5281 ttcaccagtg agacgggcaa cagctgattg cccttcaccg cctggccctg agagagttgc
     5341 agcaagcggt ccacgctggt ttgccccagc aggcgaaaat cctgtttgat ggtggttaac
     5401 ggcgggatat aacatgagct gtcttcggta tcgtcgtatc ccactaccga gatatccgca
     5461 ccaacgcgca gcccggactc ggtaatggcg cgcattgcgc ccagcgccat ctgatcgttg
     5521 gcaaccagca tcgcagtggg aacgatgccc tcattcagca tttgcatggt ttgttgaaaa
     5581 ccggacatgg cactccagtc gccttcccgt tccgctatcg gctgaatttg attgcgagtg
     5641 agatatttat gccagccagc cagacgcaga cgcgccgaga cagaacttaa tgggcccgct
     5701 aacagcgcga tttgctggtg acccaatgcg accagatgct ccacgcccag tcgcgtaccg
     5761 tcttcatggg agaaaataat actgttgatg ggtgtctggt cagagacatc aagaaataac
     5821 gccggaacat tagtgcaggc agcttccaca gcaatggcat cctggtcatc cagcggatag
     5881 ttaatgatca gcccactgac gcgttgcgcg agaagattgt gcaccgccgc tttacaggct
     5941 tcgacgccgc ttcgttctac catcgacacc accacgctgg cacccagttg atcggcgcga
     6001 gatttaatcg ccgcgacaat ttgcgacggc gcgtgcaggg ccagactgga ggtggcaacg
     6061 ccaatcagca acgactgttt gcccgccagt tgttgtgcca cgcggttggg aatgtaattc
     6121 agctccgcca tcgccgcttc cactttttcc cgcgttttcg cagaaacgtg gctggcctgg
     6181 ttcaccacgc gggaaacggt ctgataagag acaccggcat actctgcgac atcgtataac
     6241 gttactggtt tcacattcac caccctgaat tgactctctt ccgggcgcta tcatgccata
     6301 ccgcgaaagg ttttgcgcca ttcgatggtg tccgggatct cgacgctctc ccttatgcga
     6361 ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag
     6421 gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat
     6481 acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt
     6541 gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat
     6601 gcgtccggcg tagaggatcg agatctcgat cccgcgaaat taatacgact cactatagg
//
