LOCUS       sequence-236196-        8029 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression Brg1 amino acids 1-837 in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8029)
  AUTHORS   Zhou J, Zhang M, Fang H, El-Mounayri O, Rodenberg JM, Imbalzano AN, 
            Herring BP
  TITLE     The SWI/SNF chromatin remodeling complex regulates myocardin-induced
            smooth muscle-specific gene expression.
  JOURNAL   Arterioscler Thromb Vasc Biol. 2009 Jun;29(6):921-8. doi: 
            10.1161/ATVBAHA.109.187229. Epub 2009 Apr 2.
  PUBMED    19342595
REFERENCE   2  (bases 1 to 8029)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1161/ATVBAHA.109.187229"; journalName: "Arterioscler Thromb Vasc
            Biol"; date: "2009-06"; volume: "29"; issue: "6"; pages: "921-8"
COMMENT     Sequence Label: Brg1 Del2 pET28a
FEATURES             Location/Qualifiers
     source          1..8029
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             1..23
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             42..59
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             69..86
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             90..122
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             2836..2853
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(2906..2924)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      2920..2967
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      3004..3459
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3091..3110)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3301..3322
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(3551..4366)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(3551..4366)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     4274..4293
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     4358..4377
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      4488..5076
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4977..4996
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5230..5247
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    5262..5401
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(5327..5346)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5487..5509
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(5503..5694)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(6503..7585)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     7547..7566
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(7586..7663)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     7869..7888
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     7972..7991
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7972..7990
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    7991..8015
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 tttgtttaac tttaagaagg agatatacca tgggcagcag ccatcatcat catcatcaca
       61 gcagcggcct ggtgccgcgc ggcagccata tggctagcat gactggtgga cagcaaatgg
      121 gtcgcggatc cggtggtggt gcaaatcaaa gaactgctcc tcagtggatg ttgcctttac
      181 ttctaggcct gtacggaagt gttacttctg ctctaaaagc tgctctagcc ctcgacggcg
      241 ccggcggctc ctgcaggagg ccactgtctg cagctcccgt gaagatgtcc actccagacc
      301 cacccctggg cggaactcct cggccaggtc cttccccggg ccctggccct tcccctggag
      361 ccatgctggg ccctagcccg ggtccctcgc cgggctccgc ccacagcatg atggggccca
      421 gcccagggcc gccctcagca ggacacccca tccccaccca ggggcctgga gggtaccctc
      481 aggacaacat gcaccagatg cacaagccca tggagtccat gcatgagaag ggcatgtcgg
      541 acgacccgcg ctacaaccag atgaaaggaa tggggatgcg gtcagggggc catgctggga
      601 tggggccccc gcccagcccc atggaccagc actcccaagg ttacccctcg cccctgggtg
      661 gctctgagca tgcctctagt ccagttccag ccagtggccc gtcttcgggg ccccagatgt
      721 cttccgggcc aggaggtgcc ccgctggatg gtgctgaccc ccaggccttg gggcagcaga
      781 accggggccc aaccccattt aaccagaacc agctgcacca gctcagagct cagatcatgg
      841 cctacaagat gctggccagg gggcagcccc tccccgacca cctgcagatg gcggtgcagg
      901 gcaagcggcc gatgcccggg atgcagcagc agatgccaac gctacctcca ccctcggtgt
      961 ccgcaacagg acccggccct ggccctggcc ctggccccgg cccgggtccc ggcccggcac
     1021 ctccaaatta cagcaggcct catggtatgg gagggcccaa catgcctccc ccaggaccct
     1081 cgggcgtgcc ccccgggatg ccaggccagc ctcctggagg gcctcccaag ccctggcctg
     1141 aaggacccat ggcgaatgct gctgccccca cgagcacccc tcagaagctg attcccccgc
     1201 agccaacggg ccgcccttcc cccgcgcccc ctgccgtccc acccgccgcc tcgcccgtga
     1261 tgccaccgca gacccagtcc cccgggcagc cggcccagcc cgcgcccatg gtgccactgc
     1321 accagaagca gagccgcatc acccccatcc agaagccgcg gggcctcgac cctgtggaga
     1381 tcctgcagga gcgcgagtac aggctgcagg ctcgcatcgc acaccgaatt caggaacttg
     1441 aaaaccttcc cgggtccctg gccggggatt tgcgaaccaa agcgaccatt gagctcaagg
     1501 ccctcaggct gctgaacttc cagaggcagc tgcgccagga ggtggtggtg tgcatgcgga
     1561 gggacacagc gctggagaca gccctcaatg ctaaggccta caagcgcagc aagcgccagt
     1621 ccctgcgcga ggcccgcatc actgagaagc tggagaagca gcagaagatc gagcaggagc
     1681 gcaagcgccg gcagaagcac caggaatacc tcaatagcat tctccagcat gccaaggatt
     1741 tcaaggaata tcacagatcc gtcacaggca aaatccagaa gctgaccaag gcagtggcca
     1801 cgtaccatgc caacacggag cgggagcaga agaaagagaa cgagcggatc gagaaggagc
     1861 gcatgcggag gctcatggct gaagatgagg aggggtaccg caagctcatc gaccagaaga
     1921 aggacaagcg cctggcctac ctcttgcagc agacagacga gtacgtggct aacctcacgg
     1981 agctggtgcg gcagcacaag gctgcccagg tcgccaagga gaaaaagaag aaaaagaaaa
     2041 agaagaaggc agaaaatgca gaaggacaga cgcctgccat tgggccggat ggcgagcctc
     2101 tggacgagac cagccagatg agcgacctcc cggtgaaggt gatccacgtg gagagtggga
     2161 agatcctcac aggcacagat gcccccaaag ccgggcagct ggaggcctgg ctcgagatga
     2221 acccggggta tgaagtagct ccgaggtctg atagtgaaga aagtggctca gaagaagagg
     2281 aagaggagga ggaggaagag cagccgcagg cagcacagcc tcccaccctg cccgtggagg
     2341 agaagaagaa gattccagat ccagacagcg atgacgtctc tgaggtggac gcgcggcaca
     2401 tcattgagaa tgccaagcaa gatgtcgatg atgaatatgg cgtgtcccag gcccttgcac
     2461 gtggcctgca gtcctactat gccgtggccc atgctgtcac tgagagagtg gacaagcagt
     2521 cagcgcttat ggtcaatggt gtcctcaaac agtaccagat caaaggtttg gagtggctgg
     2581 tgtccctgta caacaacaac ctgaacggca tcctggccga cgagatgggc ctggggaaga
     2641 ccatccagac catcgcgctc atcacgtacc tcatggagca caaacgcatc aatgggccct
     2701 tcctcatcat cgtgcctctc tcaacgctgt ccaactgggc gtacgagttt gacaagtggg
     2761 ccccctccgt ggtgaaggtg tcttacaagg gatccgaatt cgagctccgt cgacaagctt
     2821 gcggccgcac tcgagcacca ccaccaccac cactgagatc cggctgctaa caaagcccga
     2881 aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc
     2941 tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg attggcgaat
     3001 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     3061 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     3121 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     3181 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     3241 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     3301 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     3361 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     3421 ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa
     3481 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     3541 gaattaattc ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta ttcatatcag
     3601 gattatcaat accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa aactcaccga
     3661 ggcagttcca taggatggca agatcctggt atcggtctgc gattccgact cgtccaacat
     3721 caatacaacc tattaatttc ccctcgtcaa aaataaggtt atcaagtgag aaatcaccat
     3781 gagtgacgac tgaatccggt gagaatggca aaagtttatg catttctttc cagacttgtt
     3841 caacaggcca gccattacgc tcgtcatcaa aatcactcgc atcaaccaaa ccgttattca
     3901 ttcgtgattg cgcctgagcg agacgaaata cgcgatcgct gttaaaagga caattacaaa
     3961 caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc atcaacaata ttttcacctg
     4021 aatcaggata ttcttctaat acctggaatg ctgttttccc ggggatcgca gtggtgagta
     4081 accatgcatc atcaggagta cggataaaat gcttgatggt cggaagaggc ataaattccg
     4141 tcagccagtt tagtctgacc atctcatctg taacatcatt ggcaacgcta cctttgccat
     4201 gtttcagaaa caactctggc gcatcgggct tcccatacaa tcgatagatt gtcgcacctg
     4261 attgcccgac attatcgcga gcccatttat acccatataa atcagcatcc atgttggaat
     4321 ttaatcgcgg cctagagcaa gacgtttccc gttgaatatg gctcataaca ccccttgtat
     4381 tactgtttat gtaagcagac agttttattg ttcatgacca aaatccctta acgtgagttt
     4441 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     4501 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     4561 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     4621 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     4681 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     4741 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     4801 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     4861 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     4921 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     4981 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     5041 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     5101 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     5161 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     5221 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc
     5281 cttacgcatc tgtgcggtat ttcacaccgc aatggtgcac tctcagtaca atctgctctg
     5341 atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc
     5401 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     5461 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     5521 atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc
     5581 acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt
     5641 ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc
     5701 ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat
     5761 gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa
     5821 acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg
     5881 cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat
     5941 ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa
     6001 accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca
     6061 cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag
     6121 ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggggccgcca tgccggcgat
     6181 aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag
     6241 ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa
     6301 gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat
     6361 aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct
     6421 gactgggttg aaggctctca agggcatcgg tcgagatccc ggtgcctaat gagtgagcta
     6481 acttacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     6541 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgccaggg
     6601 tggtttttct tttcaccagt gagacgggca acagctgatt gcccttcacc gcctggccct
     6661 gagagagttg cagcaagcgg tccacgctgg tttgccccag caggcgaaaa tcctgtttga
     6721 tggtggttaa cggcgggata taacatgagc tgtcttcggt atcgtcgtat cccactaccg
     6781 agatatccgc accaacgcgc agcccggact cggtaatggc gcgcattgcg cccagcgcca
     6841 tctgatcgtt ggcaaccagc atcgcagtgg gaacgatgcc ctcattcagc atttgcatgg
     6901 tttgttgaaa accggacatg gcactccagt cgccttcccg ttccgctatc ggctgaattt
     6961 gattgcgagt gagatattta tgccagccag ccagacgcag acgcgccgag acagaactta
     7021 atgggcccgc taacagcgcg atttgctggt gacccaatgc gaccagatgc tccacgccca
     7081 gtcgcgtacc gtcttcatgg gagaaaataa tactgttgat gggtgtctgg tcagagacat
     7141 caagaaataa cgccggaaca ttagtgcagg cagcttccac agcaatggca tcctggtcat
     7201 ccagcggata gttaatgatc agcccactga cgcgttgcgc gagaagattg tgcaccgccg
     7261 ctttacaggc ttcgacgccg cttcgttcta ccatcgacac caccacgctg gcacccagtt
     7321 gatcggcgcg agatttaatc gccgcgacaa tttgcgacgg cgcgtgcagg gccagactgg
     7381 aggtggcaac gccaatcagc aacgactgtt tgcccgccag ttgttgtgcc acgcggttgg
     7441 gaatgtaatt cagctccgcc atcgccgctt ccactttttc ccgcgttttc gcagaaacgt
     7501 ggctggcctg gttcaccacg cgggaaacgg tctgataaga gacaccggca tactctgcga
     7561 catcgtataa cgttactggt ttcacattca ccaccctgaa ttgactctct tccgggcgct
     7621 atcatgccat accgcgaaag gttttgcgcc attcgatggt gtccgggatc tcgacgctct
     7681 cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc
     7741 gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg
     7801 cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct
     7861 tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg
     7921 ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa ttaatacgac
     7981 tcactatagg ggaattgtga gcggataaca attcccctct agaaataat
//
