LOCUS       sequence-236208-        1159 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression plasmid for PURE system components.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Shimizu Y, Inoue A, Tomari Y, Suzuki T, Yokogawa T, Nishikawa K, 
            Ueda T
  TITLE     Cell-free translation reconstituted with purified components.
  JOURNAL   Nat Biotechnol. 2001 Aug;19(8):751-5. doi: 10.1038/90802.
  PUBMED    11479568
REFERENCE   2  (bases 1 to 1159)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Biotechnol"; date: "2001-08"; volume: "19"; issue: "8"; pages: 
            "751-5"
COMMENT     Sequence Label: pQE30-His-AsnRS
FEATURES             Location/Qualifiers
     source          1..1159
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(1..35)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag ttctgaggtc
       61 attactggat ctatcaacag gagtccaagc tcagctaatt aagcttttag aagctggcgt
      121 tacgcggagt acgtgggaac ggaatcacat cacgtacgtt ttgcacgcca gttacgtaag
      181 caatcagacg ttcaaaacca agaccgaaac ctgaatgcgg aacagtaccg tagcgacgca
      241 gatcgcgata ccaccagtaa tcttctttat tcaggcccat ttccagcata cgctcgtcca
      301 gcacgtccag acgttcttca cgctgggagc caccaatgat ctcaccgatg cccggagcca
      361 gaacgtccat agccgcaacg gttttaccgt cttcgttaag gcgcatatag aacgctttaa
      421 tatctttcgg atagttttta accactaccg gtgctttaaa gtgttcttcc gccagataac
      481 gctcatgctc agaagagaga tcgactcccc agtaaaccgg gttttcaaac ttcctgccgc
      541 agttttcgag aatggtcact gcgtcggtat aatccacctg cgcaaaatcg gcttcaatga
      601 agcgttccag acgtgaaacg gcatctttat ctacgcgttc agcgaagaat ttcatgtcgt
      661 cagcgcgttc ttcgagaacc gctttgaaga catatttcag catggcttca gccagacccg
      721 caatatcgtt caggttagca aacgccactt ccggctccag catccagaat tccgccaggt
      781 gacggctggt gttggagttt tcagcacgga aagtcgggcc gaaggtataa attttggaca
      841 atgcgcaagc gtaggtttcg ccgttcaact ggccagatac ggtcaggaaa gactctttac
      901 caaagaagtc tttgtcgaaa tccactttgc cctgatcgtt acgcggcagg ttttccagat
      961 ccagcgtaga aacgcggaac atttcgcctg caccttcggt atcagatgcg gtaatcagtg
     1021 gcgttgaaac ccagaagaat ccctgctcgt taaagaagcg atgcagcgcc tgcgccagcg
     1081 tatggcgaac gcgcgcgacg gcaccaatca ggtttgtgcg cggacgcagg tgagcgactt
     1141 cacgcaatac tcaatgctg
//
