LOCUS       sequence-236226-        1062 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression plasmid for PURE system components.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Shimizu Y, Inoue A, Tomari Y, Suzuki T, Yokogawa T, Nishikawa K, 
            Ueda T
  TITLE     Cell-free translation reconstituted with purified components.
  JOURNAL   Nat Biotechnol. 2001 Aug;19(8):751-5. doi: 10.1038/90802.
  PUBMED    11479568
REFERENCE   2  (bases 1 to 1062)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Biotechnol"; date: "2001-08"; volume: "19"; issue: "8"; pages: 
            "751-5"
COMMENT     Sequence Label: pET21a-LysRS-His
FEATURES             Location/Qualifiers
     source          1..1062
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             36..58
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ttgtgagcgg ataanaattc ccctctagaa ataattttgt ttaactttaa gaaggagata
       61 tacatatgtc tgaacaagaa acacggggag ccaatgaggc tattgatttt aacgatgaac
      121 tgagaaatcg ccgcgaaaaa ctggcggcac tacgtcagca aggtgtggcg tttcccaatg
      181 attttcgccg cgaccatacc tctgaccagt tgcacgaaga gtttgatgcg aaggataacc
      241 aggaactgga atccttaaac attgaagtct cggttgctgg ccgaatgatg acccgtcgta
      301 tcatggggaa agcctccttt gtaacgttgc aggatgtcgg tggmcgtatt caactgtacg
      361 ttgcaagaga tagcctgcca gaaggtgttt ataacgatca gtttaaaaaa tgggatctgg
      421 gtgacattat cggtgcccgc ggtacgctgt ttaagacgca ancgggtgag ctttccattc
      481 actgtactga gctgcgcctg ctgactaaag cactacgtcc tttaccagat aaattccatg
      541 gtctgcagga tcaggaagtc cgttatcgtc aacgttatct ggacctcatc gctaacgata
      601 aatcccgtca aacgtttgtt gtccgttcaa aaattctggc cgctatccgt caattcatgg
      661 tcgcgcgcgg ctttatggaa gtagaaaccc cgatgatgca ggtaattcca ggtggggcat
      721 ctgctcgccc gtttattacc catcataatg ctctggattt agatatgtat ctgcgtatcg
      781 cgccggagct gtatctgaaa cgtctggttg taggcggttt tgaacgggta ttcgaaatca
      841 accgtaactt ccgtaatgaa ggtatttctg ttcgccataa tcctgagttc acaatgatgg
      901 aactctacat ggcgtatgcg gattaccacg atttgattga actgacagag tcactgttcc
      961 gcaccctggc acaagaggtt ctgggtacca ctaaagtcac ttatggcgag catgtgtttg
     1021 atttcggcaa accgtttgaa aaactcacca tgcgcgaagc aa
//
