LOCUS       sequence-236227-         925 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression plasmid for PURE system components.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Shimizu Y, Inoue A, Tomari Y, Suzuki T, Yokogawa T, Nishikawa K, 
            Ueda T
  TITLE     Cell-free translation reconstituted with purified components.
  JOURNAL   Nat Biotechnol. 2001 Aug;19(8):751-5. doi: 10.1038/90802.
  PUBMED    11479568
REFERENCE   2  (bases 1 to 925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Biotechnol"; date: "2001-08"; volume: "19"; issue: "8"; pages: 
            "751-5"
COMMENT     Sequence Label: pET21a-MetRS-His
FEATURES             Location/Qualifiers
     source          1..925
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     29..47
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(100..117)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gacccgttta gaggccccaa ggggttatgc tagttattgc tcagcggtgg cagcagccaa
       61 ctcagcttcc tttcgggctt tgttagcagc cggatctcag tggtggtggt ggtggtgctc
      121 gagtttcacc tgatgacccg gtttagcacc ggcatccggg cttagcagga aaatatcttt
      181 cccgccagga ccggcagcca tcaccatgcc ttcagagata ccgaagcgca ttttacgtgg
      241 tgccaggtta gccaccataa tggtgtgacg accaatcagt gcctgcggat ccgggtaagc
      301 agaacgaata ccggagaaga cattgcgttt ttcaccgccg agatccagcg tcaggcgcag
      361 cagtttgtca gaaccttcaa caaactctgc gttttcaatc agcgccacgc gcaggtcaac
      421 tttagcgaag tcgtcaaagg tgatggtttc ctgaatcgga tcatctgcca gcgggccagt
      481 taccggcgcg gcagcggctt ttacttcttc tttagaggct tccaccagtg cttcaacctg
      541 cctcatatcg atgcggttat acagcgcctt gaacggattc actttgtggc ccagcagcgg
      601 ttgctggata ccatcccagg tcagttccgt attgaggaat gcttctgcac gctcggtcag
      661 tttcggcagt accggcttca ggtaagtcat cagcacgcgg aacaggttga tgcccattga
      721 gcaaattgcc tgcaggtcgg catcgcggcc ttcctgtttc gccaccaccc acggagcctg
      781 ttcatcgaca tagcggttag ccagatcagc cagcgccatg atttcgcgca cggctttacc
      841 aaattcacgg ctttcccacg cttcaccaat cacttcagcg gcatcagtga acgttttgta
      901 caactgcggg tcagccagtt cgctt
//
