LOCUS       sequence-236228-        1071 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression plasmid for PURE system components.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Shimizu Y, Inoue A, Tomari Y, Suzuki T, Yokogawa T, Nishikawa K, 
            Ueda T
  TITLE     Cell-free translation reconstituted with purified components.
  JOURNAL   Nat Biotechnol. 2001 Aug;19(8):751-5. doi: 10.1038/90802.
  PUBMED    11479568
REFERENCE   2  (bases 1 to 1071)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Biotechnol"; date: "2001-08"; volume: "19"; issue: "8"; pages: 
            "751-5"
COMMENT     Sequence Label: pET21a-MetRS-His
FEATURES             Location/Qualifiers
     source          1..1071
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    9..25
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ataggggatt gtgagcggat aacaattccc ctctagaagg agatcctact atgactcaag
       61 tcgcgaagaa aattctggtg acgtgcgcac tgccgtacgc taacggctca atccacctcg
      121 gccatatgct ggagcacatc cnggctgatg tctgggtccg ttaccagcga atgcgcggcc
      181 acgaggtcaa cttcatctgc gccgacaatg cccacggtac accgatcatg ctgaaagctc
      241 agcagcttgg tatcaccccg gagcagatga ttggcgaaat gagtcaggag catcagactg
      301 atttcgcagg ctttaacatc agctatgaca actatcactc gacgcacagc gaagagaacc
      361 gccngttgtc agaacttatc tactctcgcc tgaaagaaaa cggttttatt aaaaaccgca
      421 ccatctctca gctgtacgat ccggaaaaag gcatgttcct gccggaccgt tttgtgaaag
      481 gcacctgccc gaaatgtaaa tccccggatc aatacggcga tanctgcgaa gtctgcggcg
      541 cgacctacag cccgactgaa ctgatcgagc cgaaatcggt ggtttctggc gctacgccgg
      601 tantgcgtga ttctgaacac ttcttctttg atctgccctc tttcagcgaa atgttgcagg
      661 catggacccg cagcggtgcg ttgcaggagc aggtggcaaa taaaatgcag gagtggtttg
      721 aatctggcct gcaacagtgg gatatctccc gcgacgcccc ttacttcggt tttgaaattc
      781 cgaacgcgcc gggcaaatat ttctacgtct ggctggacgc accgattggc tacatgggtt
      841 ctttcaagaa tctgtgcgac aagcgcggcg acngcgtaag cttcgatgaa tactggaaga
      901 aagactccac cgccgagctg taccacttca tcggtaaaga tattgtttac ttccacagcc
      961 tgttctggcc tgccatgctg gaaggcagca acttccgcaa gccgtccaac ctgtttgttc
     1021 atggctatgt gacggtgaac ggcgcaaaga tgtccaagtc tcgcggcacn t
//
