LOCUS       sequence-236235-        1264 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression plasmid for PURE system components.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Shimizu Y, Inoue A, Tomari Y, Suzuki T, Yokogawa T, Nishikawa K, 
            Ueda T
  TITLE     Cell-free translation reconstituted with purified components.
  JOURNAL   Nat Biotechnol. 2001 Aug;19(8):751-5. doi: 10.1038/90802.
  PUBMED    11479568
REFERENCE   2  (bases 1 to 1264)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Biotechnol"; date: "2001-08"; volume: "19"; issue: "8"; pages: 
            "751-5"
COMMENT     Sequence Label: pQE30-His-ThrRS
FEATURES             Location/Qualifiers
     source          1..1264
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    11..27
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    43..59
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             78..89
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             108..125
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             132..146
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
ORIGIN
        1 tttatttgct ttgtgagcgg ataacaatta taatagattc aattgtgagc ggataacaat
       61 ttcacacaga attcattaaa gaggagaaat taactatgag aggatcgcat caccatcacc
      121 atcacggatc cgatgacgat gacaaacctg ttataactct tcctgatggc agccaacgcc
      181 attacgatca cgctgtaagc cccatggatg ttgcgctgga cattggtcca ggtctggcga
      241 aagcctgtat cgcagggcgc gttaatggcg aactggttga tgcttgcgat ctgattgaaa
      301 acgacgcaca actgtcgatc attaccgcca aagacgaaga aggtctggag atcattcgtc
      361 actcctgtgc gcacctgtta gggcacgcga ttaaacaact ttggccgcat accaaaatgg
      421 caatcggccc ggttattgac aacggttttt attacgacgt tgatcttgac cgcacgttaa
      481 cccaggaaga tgtcgaagca ctcgagaagc ggatgcatga gcttgctgag aaaaactacg
      541 acgtcattaa gaagaaagtc agctggcacg aagcgcgtga aactttcgcc aaccgtgggg
      601 agagctacaa agtctccatt cttgacgaaa acatcgccca tgatgacaag ccaggtctgt
      661 acttccatga agaatatgtc gatatgtgcc gcggtccgca cgtaccgaac atgcgtttct
      721 gccatcattt caaactaatg aaaacggcag gggcttactg gcgtggcgac agcaacaaca
      781 aaatgttgca acgtatttac ggtacggcgt gggcagacaa aaaagcactt aacgcttacc
      841 tgcagcgcct ggaagaagcc gcgaaacgcg accaccgtaa aatcggtaaa cagctcgacc
      901 tgtaccatat gcaggaagaa gcgccgggta tggtattctg gcacaacgac ggctggacca
      961 tcttccgtga actggaagtg tttgttcgtt ctaaactgaa agagtaccag tatcaggaag
     1021 ttaaaggtcc gttcatgatg gaccgtgtcc tgtgggaaaa aaccggtcac tgggacaact
     1081 acaaagatgc aatgttcmcc acatcttctg araaccgtga atactgcatt aagccgatga
     1141 actgcccggg tcmcgtacaa attttcaacc angggctgaa gtcttatcsc gatctgccgc
     1201 tgcctatggc ccaatttggt aactgccacc gtaacgagcc gtcaggttcc ctgcaggcct
     1261 gatg
//
