LOCUS       sequence-236236-        1193 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression plasmid for PURE system components.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Shimizu Y, Inoue A, Tomari Y, Suzuki T, Yokogawa T, Nishikawa K, 
            Ueda T
  TITLE     Cell-free translation reconstituted with purified components.
  JOURNAL   Nat Biotechnol. 2001 Aug;19(8):751-5. doi: 10.1038/90802.
  PUBMED    11479568
REFERENCE   2  (bases 1 to 1193)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Biotechnol"; date: "2001-08"; volume: "19"; issue: "8"; pages: 
            "751-5"
COMMENT     Sequence Label: pQE30-His-ThrRS
FEATURES             Location/Qualifiers
     source          1..1193
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(1..34)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt tctgaggtca
       61 ttactggatc tatcaacagg agtccaagct cagctaatta agcttttatt cctccaattg
      121 tttaagactg cggctgcgaa tctcttgttg cagcttctcg atcacttcat ttacgtccat
      181 gcttcccagg tctttaccac ggcgggtgcg aacggcaact ttgcctgatt ccacctcttt
      241 atcaccacag accagcatat atgggacgcg acgcaaagtg tgctcgcgga ttttaaagcc
      301 aatcttctca tttctcaagt ctgctttaac acgaatgccc gcatttgata gtttttgcgt
      361 caattcgtta acgtattcag actgtgaatc ggtaatattc atgataacaa cctgaaccgg
      421 cgcaagccag gtcgggaaga aaccagcgaa ctcttcggtc aggataccga tgaaacgttc
      481 catcgacccc agaattgcgc ggtgaatcat taccggtact ttacgttcat tgtcttcgcc
      541 tacataagaa gcgctcagac gagacggcaa agagaagtcc agctgtactg taccgcactg
      601 ccatgcacga tcgaggcagt catacagggt aaattcaatt ttcggaccgt agaaagcgcc
      661 ttcacccagt tgatattcaa acgggatgtt gttttcttcc agcgcaaccg ccaggtccgc
      721 ctcagcacga tcccacattt cgtcgctgcc aatacgtttt tcaggacgag tggagagttt
      781 gacgacgatc ttctcgaagc caaaagtgct gtacatatca tagactaaac ggatacatcc
      841 gttaacttca tcgcgaattt gttcttcagt acagaagata tgcgcgtcat cctgggtaaa
      901 tccacgcacg cgcatcaggc catgcagcga acctgacggc tcgttacggt ggcagctacc
      961 aaactcggcc atacgcagcg gcagatcgcg ataagacttc agcccctggt tgaaantttg
     1021 tacgtgaccc gggcagttca tcggcttaat gcagtattca cggttctcag aagagntggt
     1081 gaacattgca tctttgtagt tgtcccagtg accggttttt tcccacagga cncggtccat
     1141 catgaacgga cctttaactt cctgaaacgg ggactctttc agttaaaacg aac
//
