LOCUS       sequence-236239-        1235 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression plasmid for PURE system components.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Shimizu Y, Inoue A, Tomari Y, Suzuki T, Yokogawa T, Nishikawa K, 
            Ueda T
  TITLE     Cell-free translation reconstituted with purified components.
  JOURNAL   Nat Biotechnol. 2001 Aug;19(8):751-5. doi: 10.1038/90802.
  PUBMED    11479568
REFERENCE   2  (bases 1 to 1235)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Biotechnol"; date: "2001-08"; volume: "19"; issue: "8"; pages: 
            "751-5"
COMMENT     Sequence Label: pET21a-TyrRS-His
FEATURES             Location/Qualifiers
     source          1..1235
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..67
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(120..137)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cctttcgcaa aacccctcaa gacccgttta gaggccccaa ggggttatgc tagttattgc
       61 tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt tgttagcagc cggatctcag
      121 tggtggtggt ggtggtgctc gagtttccag caaatcagac agtaattctt tttaccgcga
      181 cgcagtaagg taaaacgacc aaacagacga tcttcttctt taaagaagta ttcaggatcg
      241 gactgttttt caccgttaat ggtgatggca ttggaggcga tagttttacg tgcctgacca
      301 cgggaaggtt gcagttcaga atcgaccagt gcctgcatca ggtctgcgcc cttttccatc
      361 tcaaccatcg gtacgccgtc ctgcgccagc tgttcgaagt ccgcttcact cagcgcactc
      421 aaagaaccgc tgaacaggca ttcggtaata cgttttgccg cctgtaaacc ttcttcaccg
      481 tgaaccagac gagtcacctg ctccgccagt acatactggg cgcgcggtgc tttaccgctg
      541 tttttatctt cttcttccag ggcgttgatc tcttcaatgc tcataaaggt gaagaacttc
      601 aggaagcggt aaacgtcggc atccgcagtg ttgatccaga actggtagaa tttgtacggg
      661 ctggttttct tcggatccaa ccagactgcg ccgccttcag ttttaccaaa tttggtgcca
      721 tctgctttag tgatcagcgg aacggtcagg ccaaacacct gattctgatg cagacgacgg
      781 gtcaggtcga taccagaagt gatgttaccc cactggtcag aaccaccaat ttgcagcacc
      841 acaccgtact gtttgttcag acaggcgaag tcataaccct gcaacaggtt gtaggaaaac
      901 tcagtgaacg aaatcccctg atcttcacgg ttgagacgct gcttaaccgc ttctttgttg
      961 atcatctggt taacggagaa gtgtttgcca atatcgcgca ggaaggtcag cacattcata
     1021 ttgccgaacc agtcatagtt gttcgccgcg atagcagagt tttctccmca gtcgaaatcg
     1081 aggaacgggg caacctgctt acggattttg tccacccact cctgaacagt ttcttcggtg
     1141 ttcagcttac sctcggcagc tttgaagctc ggntcgccaa tcagacccgt ccsgccgcct
     1201 accagcsnaa ccggcttgkg gcccgcctgc tggaa
//
