LOCUS       sequence-236240-        1081 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression plasmid for PURE system components.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Shimizu Y, Inoue A, Tomari Y, Suzuki T, Yokogawa T, Nishikawa K, 
            Ueda T
  TITLE     Cell-free translation reconstituted with purified components.
  JOURNAL   Nat Biotechnol. 2001 Aug;19(8):751-5. doi: 10.1038/90802.
  PUBMED    11479568
REFERENCE   2  (bases 1 to 1081)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Biotechnol"; date: "2001-08"; volume: "19"; issue: "8"; pages: 
            "751-5"
COMMENT     Sequence Label: pET21a-TyrRS-His
FEATURES             Location/Qualifiers
     source          1..1081
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    11..27
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             46..68
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ctatagggga ttgtgagcgg ataacaattc ccctctagaa ataattttgt ttaactttaa
       61 gaaggagata tacatatggc aagcagtaac ttgattaaac aattgcaaga gcgggggctg
      121 gtagcccagg tgacggacga ggaagcgtta gcagagcgac tggcgcaagg cccgatcgcg
      181 ctctattgcg gcttcgatcc taccgctgac agcttgcatt tggggcatct tgttccattg
      241 ttatgcctga aacgcttcca gcaggcgggc cacaagccgg ttgcgctggt aggcggcgcg
      301 acgggtctga ttggcgaccc gagcttcaaa gctgccgagc gtaagctgaa caccgaagaa
      361 actgttcagg agtgggtgga caaaatccgt aagcaggttg ccccgttcct cgatttcgac
      421 tgtggagaaa actctgctat cgcggcgaac aactatgact ggttcggcaa tatgaatgtg
      481 ctgaccttcc tgcgcgatat tggcaaacac ttctccgtta accagatgat caacaaagaa
      541 gcggttaagc agcgtctcaa ccgtgaagat caggggattt cgttcactga gttttcctac
      601 aacctgttgc agggttatga cttcgcctgt ctgaacaaac agtacggtgt ggtgctgcaa
      661 attggtggtt ctgaccagtg gggtaacatc acttctggta tcgacctgac ccgtcgtctg
      721 catcagaatc aggtgtttgg cctgaccgtt ccgctgatca ctaaagcaga tggcaccaaa
      781 tttggtaaaa ctgaaggcgg cgcagtctgg ttggatccga agaaaaccag cccgtacaaa
      841 ttctaccagt tctggatcaa cactgcggat gccgacgttt accgcttcct gaagttcttc
      901 acctttatga gcattgaaga gatcaacgcc ctggaagaag aagataaaaa cagcggtaaa
      961 gcaccgcgcg cccagtatgt actggccgga gcaggtgact cgtctggttc acggtgaaga
     1021 aggtttacag gcggcaaaac gtattaccga atgcctgttc agcggttctt tgagtgcsct
     1081 g
//
