LOCUS       sequence-236241-        1232 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression plasmid for PURE system components.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1232)
  AUTHORS   Shimizu Y, Inoue A, Tomari Y, Suzuki T, Yokogawa T, Nishikawa K, 
            Ueda T
  TITLE     Cell-free translation reconstituted with purified components.
  JOURNAL   Nat Biotechnol. 2001 Aug;19(8):751-5. doi: 10.1038/90802.
  PUBMED    11479568
REFERENCE   2  (bases 1 to 1232)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Biotechnol"; date: "2001-08"; volume: "19"; issue: "8"; pages: 
            "751-5"
COMMENT     Sequence Label: pET21a-ValRS-His
FEATURES             Location/Qualifiers
     source          1..1232
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     40..58
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(111..128)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 aaaacccctc agacccgttt agaggcccca aggggttatg ctagttattg ctcagcggtg
       61 gcagcagcca actcagcttc ctttcgggct ttgttagcag ccggatctca gtggtggtgg
      121 tggtggtgct cgagtgcggc cgcggcgata acagcctgct gttcaatcag tttcgctttc
      181 gcttccgcat agccttccag cttctcacgc tctttcgcga tgaccgcttc cggtgcgcgg
      241 gcgacaaagc cttcgttcgc cagtttgttc tcgatacggc tgatttcacc ttcaatcttc
      301 gccacttctt tcgccagacg cgccagctca tcttctttgt tgatgaggcc agccatcggg
      361 atcagcagct ctgcaccgtc gatgatcttc gtaacggaaa ccggaccttt gtcatcggca
      421 ggcagcacgg tgatactttc cagacgcgcc agggtttgca ggaagccacg gttttcattt
      481 acgcgacgtt ctgcatccgc gctgcaacca cgcagcagca gctccagcgg tttgcccggc
      541 gcgatgttca tttctgcacg gatgttacgt accgcaacga tcgcctgttt cagccattcg
      601 gtgtcggcca gtgcggcttc atcaacctga gatgcatcgt actgcgggaa cggctgcagc
      661 atgatggtgt cggcagtgat accgcaaagt actttcacac gctgccagat ggtttcggtg
      721 atgaacggaa tgatcggatg cgcgaggcgc agcagacctt ccagtacagt caccagcgta
      781 tggcgagtac cgcgcagttc tgcttcggtg ccaccgttca ttaccggctt ggtcagctcg
      841 agataccagt cacagaactg gttccaggtg aactcataca gaatgcctgc ggcgatatcg
      901 aagcggaagc tgtccagcgc ttcgcggtac gctttgatgg tctggttgaa ctccgccaga
      961 atccagcggt ccgccagcga cagcgtcatt tcgccgccgt tgaagccgca atcctgacct
     1021 tctgtgttca tcagcacaaa gcggctggcg ttccacagct tgttacagaa gttacggtaa
     1081 ccttccagac gcttcatatc ccagttgatg tcacgaccgg tagacgccag cgccgccagg
     1141 gtgaagcgca acscgtcagt accgtgcggc tcaataccgt tcgggaactg cttcycggtg
     1201 cscttacgga ttttgtccgc agctgcggct gc
//
