LOCUS       sequence-236242-        1090 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression plasmid for PURE system components.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Shimizu Y, Inoue A, Tomari Y, Suzuki T, Yokogawa T, Nishikawa K, 
            Ueda T
  TITLE     Cell-free translation reconstituted with purified components.
  JOURNAL   Nat Biotechnol. 2001 Aug;19(8):751-5. doi: 10.1038/90802.
  PUBMED    11479568
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Biotechnol"; date: "2001-08"; volume: "19"; issue: "8"; pages: 
            "751-5"
COMMENT     Sequence Label: pET21a-ValRS-His
FEATURES             Location/Qualifiers
     source          1..1090
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    19..43
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 tatacgactc actatagggg aattgtgagc ggataacaat tcccctctag aaggagatgg
       61 aaaaatggaa aagacatata acccacaaga tatcgaacag ccgctttacg agcactggga
      121 aaagcagggc tactttaagc ctaatggcga tgaaagccag gaaagtttct gcatcatgat
      181 cccgccgccg aacgtcacng gcagtttgca tatgggtcac gccttccagc aaaccatcat
      241 ggataccatg atccgctatc agcgcatgca gggcaaaaac accctgtggc aggtcggtac
      301 tgaccacgcc gggatcgcta cccagatggt cgttgagcgc aagattgccg cagaagaagg
      361 taaaacccgt cacgactacg gccgcgaagc tttcatcgac aaaatctggg aatggaaagc
      421 ggaatctggc ggcaccatta cccgtcagat gcgccgtctc ggcaactccg tcgactggga
      481 gcgtgaacgc ttcaccatgg acgaaggcct gtccaatgcg gtgaaagaag ttttcgttcg
      541 tctgtataaa gaagacctga tttaccgtgg caaacgcctg gtaaactggg atccgaaact
      601 gcgcaccgct atctctgacc tggaagtcga aaaccgcgaa tcgaaaggtt cgatgtggca
      661 catccgctat ccgctggctg acggtgcgaa aaccgcagac ggtaaagatt atctggtggt
      721 cgcgactacc cgtccagaaa ccctgctggg cgatactggc gtagccgtta acccggaaga
      781 tccgcgttac aaagatctga ttggcaaata tgtcattctg ccgctggtta accgtcgtat
      841 tccgatcgtt ggcgacgaac acgccgacat ggaaaaaggc accggctgcg tgaaaatcac
      901 tccggcgcac gactttaacg actatgaagt gggtaaacgt cacgccctgc cgatgatcaa
      961 catcctgacc tttgacggcg atatccgtga aagcgcccag gtgttcgata ccaaaggtaa
     1021 cgaatctgac gtttattcca gcgaaatccc tgcanagttc cagaaactgg aagcgttttg
     1081 ctgcacgtaa
//
