LOCUS       sequence-236243-         876 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression plasmid for PURE system components.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Shimizu Y, Inoue A, Tomari Y, Suzuki T, Yokogawa T, Nishikawa K, 
            Ueda T
  TITLE     Cell-free translation reconstituted with purified components.
  JOURNAL   Nat Biotechnol. 2001 Aug;19(8):751-5. doi: 10.1038/90802.
  PUBMED    11479568
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Biotechnol"; date: "2001-08"; volume: "19"; issue: "8"; pages: 
            "751-5"
COMMENT     Sequence Label: pQE30-His-IF1
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    3..19
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    35..51
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             70..81
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             100..117
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      362..456
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(566..585)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 ctttgtgagc ggataacaat tataatagat tcaattgtga gcggataaca atttcacaca
       61 gaattcatta aagaggagaa attaactatg agaggatcgc atcaccatca ccatcacgga
      121 tccatggcca aagaagacaa tattgaaatg caaggtaccg ttcttgaaac gttgcctaat
      181 accatgttcc gcgtagagtt agaaaacggt cacgtggtta ctgcacacat ctccggtaaa
      241 atgcgcaaaa actacatccg catcctgacg ggcgacaaag tgactgttga actgaccccg
      301 tacgacctga gcaaaggccg cattgtcttc cgtagtcgct gaagctaatt agctgagctt
      361 ggactcctgt tgatagatcc agtaatgacc tcagaactcc atctggattt gttcagaacg
      421 ctcggttgcc gccgggcgtt ttttattggt gagaatccaa gctagcttgg cgagattttc
      481 aggagctaag gaagctaaaa tggagaaaaa aatcactgga tataccaccg ttgatatatc
      541 ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa
      601 ccagaccgtt cagctggata ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa
      661 gttttatccg gcctttattc acattcttgc ccgcctgatg aatgctcatc cggaatttcg
      721 tatggcaatg aaagacggtg agctggtgat atgggatagt gttcaccctt gttacaccgt
      781 tttccatgag caaactgaaa cgttttcatc gctctggagt gaataccacg acgatttccg
      841 gcagtttcta cacatatatt cgcaagatgt ggcgtg
//
