LOCUS       sequence-236248-        1203 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression plasmid for PURE system components.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Shimizu Y, Inoue A, Tomari Y, Suzuki T, Yokogawa T, Nishikawa K, 
            Ueda T
  TITLE     Cell-free translation reconstituted with purified components.
  JOURNAL   Nat Biotechnol. 2001 Aug;19(8):751-5. doi: 10.1038/90802.
  PUBMED    11479568
REFERENCE   2  (bases 1 to 1203)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Biotechnol"; date: "2001-08"; volume: "19"; issue: "8"; pages: 
            "751-5"
COMMENT     Sequence Label: pQE60-EFG-His
FEATURES             Location/Qualifiers
     source          1..1203
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    12..28
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    44..60
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 atttatttgc tttgtgagcg gataacaatt ataatagatt caattgtgag cggataacaa
       61 tttcacacag aattggataa acgaggaaac aaatggctcg tacaacaccc atcgcacgct
      121 accgtaacat cggtatcagt gcgcacatcg acgccggtaa aaccactact accgaacgta
      181 ttctgttcta caccggtgta aaccataaaa tcggtgaagt tcatgacggc gctgcaacca
      241 tggactggat ggagcaggag caggaacgtg gtattaccat cacttccgct gcgactactg
      301 cattctggtc tggtatggct aagcagtatg agccgcatcg catcaacatc atcgacaccc
      361 cggggcacgt tgacttcaca atcgaagtag aacgttccat gcgtgttctc gatggtgcgg
      421 taatggttta ctgcgcagtt ggtggtgttc agccgcagtc tgaaaccgta tggcgtcagg
      481 caaacaaata taaagttccg cgcattgcgt tcgttaacaa aatggaccgc atgggtgcga
      541 acttcctgaa agttgttaac cagatcaaaa cccgtctggg cgcgaacccg gttccgctgc
      601 agctggcgat tggtgctgaa gaacatttca ccggtgttgt tgacctggtg aaaatgaaag
      661 ctatcaactg gaacgacgct gaccagggcg taaccttcga atacgaagat atcccggcag
      721 acatggttga actggctaac gaatggcacc agaacctgat cgaatccgca gctgaagctt
      781 ctgaagagct gatggaaaaa tacctgggtg gtgaagaact gactgaagca gaaatcaaag
      841 gtgctctgcg acagcgcgtt ctgaacaacg aaatcatcct ggtaacctgt ggttctgcgt
      901 tcaagaacaa aggtgttcag gcgatgctgg atgcggtaat tgattacctg ccatccccgg
      961 ttgacgtacc tgcgatcaac ggtatcctgg acgacggtaa agacactccg gctgaacgtc
     1021 acgcaagtga tgacgagccg ttctctgcac tggcgttcaa aatcgctacc gacccgtttg
     1081 ttggtaacct gaccttcttc cgtgtttact ccggtgtggt taactctggt gawaccgtac
     1141 tgaactccgt gaaagctgca cgtnarcgtt tcggtcgtat cgttcagatg cmcgctaaca
     1201 aac
//
