LOCUS       sequence-236249-        1170 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression plasmid for PURE system components.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Shimizu Y, Inoue A, Tomari Y, Suzuki T, Yokogawa T, Nishikawa K, 
            Ueda T
  TITLE     Cell-free translation reconstituted with purified components.
  JOURNAL   Nat Biotechnol. 2001 Aug;19(8):751-5. doi: 10.1038/90802.
  PUBMED    11479568
REFERENCE   2  (bases 1 to 1170)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Biotechnol"; date: "2001-08"; volume: "19"; issue: "8"; pages: 
            "751-5"
COMMENT     Sequence Label: pQE60-EFG-His
FEATURES             Location/Qualifiers
     source          1..1170
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(1..34)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(107..124)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt tctgaggtca
       61 ttactggatc tatcaacagg agtccaagct cagctaatta agcttagtga tggtgatggt
      121 gatgagatct tttaccacgg gcttcaatta cggcctgagc aacgttactc ggcgcttcat
      181 catacttcag gaattccata gtgtatgatg cacgaccttt ggtcagagaa cgcagctgag
      241 ttgcgtatcc gaacatttca gacagcggta cttcagcgtg gatcttaacg ccagtaactt
      301 cagattcctg acctttgagc ataccacgac gacggctcaa gtcaccgata acgtcaccgg
      361 tgttctcttc cggagtttct acttcaacct tcatgatcgg ctcaagcaga actggtttcg
      421 ctttcttaaa gccttcttta aaggcgatag aagcagccag tttaaacgcc agttcagagg
      481 agtcaacgtc atggtaagaa ccgaagtgca gacgaatacc catgtctact accgggtagc
      541 ctgccagcgg acctgctttc agctgttcct ggataccttt atcaacggcc gggatgtatt
      601 cgccagggat tacaccacct ttaatgtcgt tgatgaactc gtagcctttc gggtttgaac
      661 ccggctccag cgggtacatg tcgataacaa catgaccata ctgaccacga ccaccagact
      721 gtttcgcgtg tttaccttca acatcggtaa ctttctggcg gatagtttca cggtaagcaa
      781 cctgcggttt acctacgttc gcttcaacgt tgaattcacg cttcatacgg tcaacgatga
      841 tgtcgaggtg cagttcgccc atacccgcga tgatggtctg gttagattct tcgtcagtcc
      901 atacacggaa agacgggtct tctttagcca gacggcccag agccagaccc attttttcct
      961 ggtcagcttt ggttttcggt tcaactgcga tggagattac cggctcaggg aattccatac
     1021 gttccagaat gatcggcgca tccgggtcac acagggtgtc accagtggtt acgtctttca
     1081 gaccgatagc agcagcgatg tcgcccgcgc gaacttcttt gatctcttca cgtttgttag
     1141 cgtgcatctg aacgatacga ccgaaacgct
//
