LOCUS       sequence-236255-        1181 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression plasmid for PURE system components.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Shimizu Y, Inoue A, Tomari Y, Suzuki T, Yokogawa T, Nishikawa K, 
            Ueda T
  TITLE     Cell-free translation reconstituted with purified components.
  JOURNAL   Nat Biotechnol. 2001 Aug;19(8):751-5. doi: 10.1038/90802.
  PUBMED    11479568
REFERENCE   2  (bases 1 to 1181)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Biotechnol"; date: "2001-08"; volume: "19"; issue: "8"; pages: 
            "751-5"
COMMENT     Sequence Label: pQE30-His-RF1
FEATURES             Location/Qualifiers
     source          1..1181
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(2..36)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt
       61 cattactgga tctatcaaca ggagtccaag ctcagctaat taagctttta ttcctgctcg
      121 gacaacgccg ccagttggtc ggcctgatgt tcctggataa tcggttcaat cagcatatcc
      181 agcttacctt ccatcacttc atccaggcgg tagagcgtca ggttgatgcg gtgatcggta
      241 acgcgcccct gcgggaagtt gtaagtacgg ttacggtcgc tgcgatcgcc actccccagc
      301 aggttacgac gggtagacgc ttcggcctgt tggcgttttg ccatttcagc agcgtggatg
      361 cgagcaccga gaacagaaag tgctttagct ttgtttttat gttgtgaacg ttcgtcctga
      421 cattcaacaa caatcccggt cggcaagtga gtaatacgaa ttgccgaatc ggtggtgtta
      481 acgtgctgac cacccgcccc tgacgagcgg aaagtatcaa tgcgtaaatc tgctgggttg
      541 atgtccggca gttctgcgtc aggcagttct ggcattaccg caacggtaca agcagaagta
      601 tgaatacgac cctgcgattc cgtagcagga acacgttgca cgcgatgacc gccggattca
      661 aatttcagac gaccatacac accatcaccg ctaattttgg cgatgatctc tttataacca
      721 ccatgttcac cctcgctggc gctcatgatt tctacccgcc agcggcgggc ttcggcataa
      781 cggctgtaca tacggaacag atcgcccgcg aacagcgccg cttcgtcgcc gccggttccg
      841 gctcggactt cgaggaaggc gttacgttcg tcatcaggat cttttggcag taacagaacc
      901 tgtaattgct gttccagttg ctcgcttttt tctttagctt cgcgcagttc atcctgcgcc
      961 atctcacgca tttcaggatc atcgagcatc atctgtgcgg tttcgatatc ttcctgaacc
     1021 tgttgccagt cggtaaaaca gcgcgaaaca tcacttaact gcgcatattc gcgtgataat
     1081 gcgcgaaaac gttcctggtc ggcgatagtt tgcgcgtcac ccagcaacgc ctgaacttct
     1141 tcatggcgtt catgcagggc ttccagtttg gcaacgatag a
//
