LOCUS       sequence-236260-         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression plasmid for PURE system components.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Shimizu Y, Inoue A, Tomari Y, Suzuki T, Yokogawa T, Nishikawa K, 
            Ueda T
  TITLE     Cell-free translation reconstituted with purified components.
  JOURNAL   Nat Biotechnol. 2001 Aug;19(8):751-5. doi: 10.1038/90802.
  PUBMED    11479568
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Biotechnol"; date: "2001-08"; volume: "19"; issue: "8"; pages: 
            "751-5"
COMMENT     Sequence Label: pQE60-RRF-His
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(34..48,49..54,55..71,72..78)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    54..70
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    86..102
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             704..721
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      794..827
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 ttycmgaggc cctttcgtct tcacctcgag aaatcataaa aaatttattt gctttgtgag
       61 cggataacaa ttataataga ttcaattgtg agcggataac aatttcacac agaattcgtt
      121 ttcaaggatt cgtaacatga ttagcgatat cagaaaagat gctgaagtac gcatggacaa
      181 atgcgtagaa gcgttcaaaa cccaaatcag caaaatacgc acgggtcgtg cttctcccag
      241 cctgctggat ggcattgtcg tggaatatta cggcacgccg acgccgctgc gtcagctggc
      301 aagcgtaacg gtagaagatt cccgtacact gaaaatcaac gtgtttgatc gttcaatgtc
      361 tccggccgtt gaaaaagcga ttatggcgtc cgatcttggc ctgaacccga actctgcggg
      421 tagcgacatc cgtgttccgc tgccgccgct gacggaagaa cgtcgtaaag atctgaccaa
      481 aatcgttcgt ggtgaagcag aacaagcgcg tgttgcagta cgtaacgtgc gtcgtgacgc
      541 gaacgacaaa gtgaaagcac tgttgaaaga taaagagatc agcgaagacg acgatcgccg
      601 ttctcaggac gatgtacaga aactgactga tgctgcaatc aagaaaattg aagcggcgct
      661 ggcagacaaa gaagcagaac tgatgcagtt cggatccaga tctcatcacc atcaccatca
      721 ctaagcttaa ttagctgagc ttggactcct gttgatagat ccagtaatga cctcagaact
      781 ccatctggat ttgttcagaa cgctcggttg ccgccgggcg tttttta
//
