LOCUS       sequence-236261-        1138 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression plasmid for PURE system components.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Shimizu Y, Inoue A, Tomari Y, Suzuki T, Yokogawa T, Nishikawa K, 
            Ueda T
  TITLE     Cell-free translation reconstituted with purified components.
  JOURNAL   Nat Biotechnol. 2001 Aug;19(8):751-5. doi: 10.1038/90802.
  PUBMED    11479568
REFERENCE   2  (bases 1 to 1138)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Biotechnol"; date: "2001-08"; volume: "19"; issue: "8"; pages: 
            "751-5"
COMMENT     Sequence Label: pET21a-MTF-His
FEATURES             Location/Qualifiers
     source          1..1138
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             42..64
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1022..1039
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(1092..1110)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 tttaggggaa tgkgagcgat aacaattccc tctagaaata atttgtttaa ctttaagaag
       61 gagatataca tatgtcagaa tcactacgta ttattttgcg ggtacacctg actttgcagc
      121 gcgtcatctc gacgcgctgt tgtcttctgg tcataacgtc gttggcgtgt tcacccagcc
      181 agaccgaccg gcaggacgcg gtaaaaaact gatgcccagc ccggttaaag ttctggctga
      241 ggaaaaaggt ctgcccgttt ttcaacctgt ttccctgcgt ccacaagaaa accagcaact
      301 ggtcgccgaa ctgcaggctg atgttatggt cgtcgtcgcc tatggtttaa ttctgccgaa
      361 agcagtgctg gagatgccgc gtcttggctg tatcaacgtt catggttcac tgctgccacg
      421 ctggcgcggt gctgcaccaa tccaacgctc actatgggcg ggtgatgcag aaactggtgt
      481 gaccattatg caaatggatg tcggtttaga caccggtgat atgctctata agctctcctg
      541 cccgattact gcagaagata ccagtggtac gctgtacgac aagctggcag agcttggccc
      601 acaagggctt atcaccacgt tgaaacaact ggcagacggc acggcgaaac cagaagttca
      661 ggacgaaact cttgtcactt acgccgagaa gttgagtaaa gaagaagcgc gtattgactg
      721 gtcactttcg gcagcacagc ttgaacgctg cattcgcgct ttcaatccat ggccaatgag
      781 ctggctggaa attgaaggac agccggttaa agtctggaaa gcatcggtca ttgatacggc
      841 aaccaacgct gcaccaggaa cgatccttga agccaacaaa caaggcattc aggttgcgac
      901 tggtgatggc atcctgaacc tgctctcgtt acaacctgcg ggtaagaaag cgatgagcgc
      961 gcaagacctc ctgaactctc gtcgggaatg gtttgttccg ggcaaccgtc tggtcctcga
     1021 gcaccaccac caccaccact gagatccggc tgctaacaaa gcccgaaagg aagctgagtt
     1081 ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtc
//
