LOCUS       sequence-236315-        9173 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  SMX_DEST vector with 3 cassettes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9173)
  AUTHORS   Sladitschek HL, Neveu PA
  TITLE     MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and 
            Flow Cytometry Approaches in Mammalian Systems.
  JOURNAL   PLoS One. 2015 Apr 24;10(4):e0124958. doi: 
            10.1371/journal.pone.0124958. eCollection 2015.
  PUBMED    25909630
REFERENCE   2  (bases 1 to 9173)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-04-24"; volume: "10(4):e0124958. doi"; pages: " 
            10.1371/journal.pone.0124958. eCollection 2015"
COMMENT     Sequence Label: ES-FUCCI
FEATURES             Location/Qualifiers
     source          1..9173
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        17..121
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(122..190,191..982)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(340..359)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1153..1741
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1642..1661
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2234..2253
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    2252..2307
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     2353..2372
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     intron          2504..3076
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     CDS             complement(3102..4127)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(3102..4127)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(4144..4643)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     polyA_signal    4655..4879
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(4889..5161)
                     /codon_start=1
                     /product="degron consisting of residues 30-120 of human 
                     Cdt1 (Zielke and Edgar, 2015)"
                     /label=Cdt1 (30-120)
                     /translation="PSPARPALRAPASATSGSRKRARPPAAPGRDQARPPARRRLRLSV
                     DEVSSPSTPEAPDIPACPSPGQKIKKSTPAAGQPPHLTSAQDQDTI"
     CDS             complement(5177..5884)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5452..5471)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     5721..5739
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      5881..5890
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(5901..6400)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     regulatory      7875..7884
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(7881..7883,7884..7886,7887..8597)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7926..7947)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8187..8206)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8534..8555
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             8604..8933
                     /codon_start=1
                     /product="degron consisting of residues 1-110 of human 
                     Geminin (Zielke and Edgar, 2015)"
                     /label=Gem1 (1-110)
                     /translation="MNPSMKQKQEEIKENIKNSSVPRRTLKMIQPSASGSLVGRENELS
                     AGLSKRKHRNDHLTSTTSSPGVIVPESSENKNLGGVTQESFDLMIKENPSSQYWKEVAE
                     KRRKAL"
     polyA_signal    8943..9167
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 aattcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
       61 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
      121 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
      181 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
      241 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
      301 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
      361 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
      421 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
      481 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
      541 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
      601 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
      661 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
      721 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
      781 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
      841 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
      901 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
      961 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     1021 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     1081 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     1141 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     1201 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     1261 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     1321 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     1381 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     1441 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     1501 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     1561 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     1621 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     1681 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     1741 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     1801 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     1861 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcc atgagtcgac
     1921 gcagtcgagt tcataagaga agagggacag ctatgactgg gagtagtcag gagaggagga
     1981 aaaatctggc tagtaaaaca tgtaaggaaa attttaggga tgttaaagaa aaaaataaca
     2041 caaaacaaaa tataaaaaaa atctaacctc aagtcaaggc ttttctatgg aataaggaat
     2101 ggacagcagg gggctgtttc atatactgat gacctcttta tagccaacct ttgttcatgg
     2161 cagccagcat atgggcatat gttgccaaac tctaaaccaa atactcattc tgatgtttta
     2221 aatgatttgc cctcccatat gtccttccga gtgagagaca caaaaaattc caacacacta
     2281 ttgcaatgaa aataaatttc ctttattagc cagaagtcag atgctcaagg ggcttcatga
     2341 tgtccccata atttttggca gagggaaaaa gatctcagtg gtatttgtga gccagggcat
     2401 tggccacacc agccaccacc ttctgatagg cagcctgcac ctgaggagtg aattctttgc
     2461 caaaatgatg agacagcaca acaaccagca cgttgcccag gagctgtagg aaagagaaga
     2521 aggcatgaac atggttagca gaggggcccg gtttggactc agagtatttt atcctcatct
     2581 caaacagtgt atatcattgt aaccataaag agaaaggcag gatgatgacc agggtgtagt
     2641 tgtttctacc aataagaata tttccacgcc agccagaatt tatatgcaga aatattctac
     2701 cttatcattt aattataaca attgttctct aaaactgtgc tgaagtacaa tataatatac
     2761 cctgattgcc ttgaaaaaaa agagattaga gaaagtactt acaatctgac aaataaacaa
     2821 aagtgaattt aaaaattcgt tacaaatgca agctaaagtt taacgaaaaa gttacagaaa
     2881 atgaaaagaa aataagagga gacaatggtt gtcaacagag tagaaagtga aagaaacaaa
     2941 attatcatga gggtccatgg tgatacaagg gacatcttcc cattctaaac aacaccctga
     3001 aaactttgcc ccctccatat aacatgaatt ttacaatagc gaaaaagaaa gaacaatcaa
     3061 gggtccccaa actcaccctg aagttctcag tctagctcga cctattcctt tgccctcgga
     3121 cgagtgctgg ggcgtcggtt tccactatcg gcgagtactt ctacacagcc atcggtccag
     3181 acggccgcgc ttctgcgggc gatttgtgta cgcccgacag tcccggctcc ggatcggacg
     3241 attgcgtcgc atcgaccctg cgcccaagct gcatcatcga aattgccgtc aaccaagctc
     3301 tgatagagtt ggtcaagacc aatgcggagc atatacgccc ggagccgcgg cgatcctgca
     3361 agctccggat gcctccgctc gaagtagcgc gtctgctgct ccatacaagc caaccacggc
     3421 ctccagaaga agatgttggc gacctcgtat tgggaatccc cgaacatcgc ctcgctccag
     3481 tcaatgaccg ctgttatgcg gccattgtcc gtcaggacat tgttggagcc gaaatccgcg
     3541 tgcacgaggt gccggacttc ggggcagtcc tcggcccaaa gcatcagctc atcgagagcc
     3601 tgcgcgacgg acgcactgac ggtgtcgtcc atcacagttt gccagtgata cacatgggga
     3661 tcagcaatcg cgcatatgaa atcacgccat gtagtgtatt gaccgattcc ttgcggtccg
     3721 aatgggccga acccgctcgt ctggctaaga tcggccgcag cgatcgcatc catggcctcc
     3781 gcgaccggct gcagaacagc gggcagttcg gtttcaggca ggtcttgcaa cgtgacaccc
     3841 tgtgcacggc gggagatgca ataggtcagg ctctcgctga attccccaat gtcaagcact
     3901 tccggaatcg ggagcgcggc cgatgcaaag tgccgataaa cataacgatc tttgtagaaa
     3961 ccatcggcgc agctatttac ccgcaggaca tatccacgcc ctcctacatc gaagctgaaa
     4021 gcacgagatt cttcgccctc cgagagctgc atcaggtcgg agacgctgtc gaacttttcg
     4081 atcagaaact tctcgacaga cgtcgcggtg agttcaggct ttttcatggt ggcgactcga
     4141 cgtcgaaagg cccggagatg aggaagagga gaacagcgcg gcagacgtgc gcttttgaag
     4201 cgtgcagaat gccgggcctc cggaggacct tcgggcgccc gccccgcccc tgagcccgcc
     4261 cctgagcccg cccccggacc caccccttcc cagcctctga gcccagaaag cgaaggagca
     4321 aagctgctat tggccgctgc cccaaaggcc tacccgcttc cattgctcag cggtgctgtc
     4381 catctgcacg agactagtga gacgtgctac ttccatttgt cacgtcctgc acgacgcgag
     4441 ctgcggggcg ggggggaact tcctgactag gggaggagta gaaggtggcg cgaaggggcc
     4501 accaaagaac ggagccggtt ggcgcctacc ggtggatgtg gaatgtgtgc gaggccagag
     4561 gccacttgtg tagcgccaag tgcccagcgg ggctgctaaa gcgcatgctc cagactgcct
     4621 tgggaaaagc gcctccccta cccggtagac tcgaccatag agcccaccgc atccccagca
     4681 tgcctgctat tgtcttccca atcctccccc ttgctgtcct gccccacccc accccccaga
     4741 atagaatgac acctactcag acaatgcgat gcaatttcct cattttatta ggaaaggaca
     4801 gtgggagtgg caccttccag ggtcaaggaa ggcacggggg aggggcaaac aacagatggc
     4861 tggcaactag aaggcacagc tcgacttaga tggtgtcctg gtcctgcgcg gatgtcaggt
     4921 ggggcggctg acctgctgcc ggggtggatt tctttatctt ctggcccgga gaagggcagg
     4981 ctgggatgtc tggggcctcg ggggtactgg ggctggaaac ctcgtccacc gacagccgca
     5041 gtctcctgcg ggccggtggc ctggcctggt cgcgtccggg ggcggcgggc gggcgggcgc
     5101 gcttgcggct gccactggta gcggaggccg gggcgcggag tgcgggcctg gcggggctgg
     5161 gctcgacatc tgtagacttg tacagctcgt ccatgccgcc ggtggagtgg cggccctcgg
     5221 cgcgttcgta ctgttccacg atggtgtagt cctcgttgtg ggaggtgatg tccaacttga
     5281 tgttgacgtt gtaggcgccg ggcagctgca cgggcttctt ggccttgtag gtggtcttga
     5341 cctcagcgtc gtagtggccg ccgtccttca gcttcagcct ctgcttgatc tcgcccttca
     5401 gggcgccgtc ctcggggtac atccgctcgg aggaggcctc ccagcccatg gtcttcttct
     5461 gcattacggg gccgtcggag gggaagttgg tgccgcgcag cttcaccttg tagatgaact
     5521 cgccgtcctg cagggaggag tcctgggtca cggtcaccac gccgccgtcc tcgaagttca
     5581 tcacgcgctc ccacttgaag ccctcgggga aggacagctt caagtagtcg ggaatgtcgg
     5641 cggggtgctt cacgtaggcc ttggagccgt acatgaactg aggggacagg atgtcccagg
     5701 cgaagggcag ggggccaccc ttggtcacct tcagcttggc ggtctgggtg ccctcgtagg
     5761 ggcggccctc gccctcgccc tcgatctcga actcgtggcc gttcacggag ccctccatgt
     5821 gcaccttgaa gcgcatgaac tccttgatga tggccatgtt atcctcctcg cccttgctca
     5881 ccatggtggc gactcgacgt cgaaaggccc ggagatgagg aagaggagaa cagcgcggca
     5941 gacgtgcgct tttgaagcgt gcagaatgcc gggcctccgg aggaccttcg ggcgcccgcc
     6001 ccgcccctga gcccgcccct gagcccgccc ccggacccac cccttcccag cctctgagcc
     6061 cagaaagcga aggagcaaag ctgctattgg ccgctgcccc aaaggcctac ccgcttccat
     6121 tgctcagcgg tgctgtccat ctgcacgaga ctagtgagac gtgctacttc catttgtcac
     6181 gtcctgcacg acgcgagctg cggggcgggg gggaacttcc tgactagggg aggagtagaa
     6241 ggtggcgcga aggggccacc aaagaacgga gccggttggc gcctaccggt ggatgtggaa
     6301 tgtgtgcgag gccagaggcc acttgtgtag cgccaagtgc ccagcggggc tgctaaagcg
     6361 catgctccag actgccttgg gaaaagcgcc tcccctaccc ggtagactcg acgcctaccc
     6421 gcttccattg ctcagcggtg ctgtccatct gcacgagact agtgagacgt gctacttcca
     6481 tttgtcacgt cctgcacgac gcgagctgcg gggcgggggg gaacttcctg actaggggag
     6541 gagtagaagg tggcgcgaag gggccaccaa agaacggagc cggttggcgc ctaccggtgg
     6601 atgtggaatg tgtgcgaggc cagaggccac ttgtgtagcg ccaagtgccc agcggggctg
     6661 ctaaagcgca tgctccagac tgccttggga aaagcgcctc ccctacccgg tagactcgac
     6721 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     6781 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     6841 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     6901 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     6961 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     7021 gactcgacgc ctacccgctt ccattgctca gcggtgctgt ccatctgcac gagactagtg
     7081 agacgtgcta cttccatttg tcacgtcctg cacgacgcga gctgcggggc gggggggaac
     7141 ttcctgacta ggggaggagt agaaggtggc gcgaaggggc caccaaagaa cggagccggt
     7201 tggcgcctac cggtggatgt ggaatgtgtg cgaggccaga ggccacttgt gtagcgccaa
     7261 gtgcccagcg gggctgctaa agcgcatgct ccagactgcc ttgggaaaag cgcctcccct
     7321 acccggtaga ctcgacgcct acccgcttcc attgctcagc ggtgctgtcc atctgcacga
     7381 gactagtgag acgtgctact tccatttgtc acgtcctgca cgacgcgagc tgcggggcgg
     7441 gggggaactt cctgactagg ggaggagtag aaggtggcgc gaaggggcca ccaaagaacg
     7501 gagccggttg gcgcctaccg gtggatgtgg aatgtgtgcg aggccagagg ccacttgtgt
     7561 agcgccaagt gcccagcggg gctgctaaag cgcatgctcc agactgcctt gggaaaagcg
     7621 cctcccctac ccggtagact cgagttacgc gtaactcgag ctttggggca gcggccaata
     7681 gcagctttgc tccttcgctt tctgggctca gaggctggga aggggtgggt ccgggggcgg
     7741 gctcaggggc gggctcaggg gcggggcggg cgcccgaagg tcctccggag gcccggcatt
     7801 ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct
     7861 ttcgacgtcg agtcgccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc
     7921 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
     7981 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
     8041 tgcccgtgcc ctggcccacc ctcgtgacca ccttcggcta cggcctgatg tgcttcgccc
     8101 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
     8161 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
     8221 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     8281 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
     8341 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg
     8401 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     8461 tgctgctgcc cgacaaccac tacctgagct accagtccgc cctgagcaaa gaccccaacg
     8521 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
     8581 tggacgagct gtacaaggtc gagatgaatc ccagtatgaa gcagaaacaa gaagaaatca
     8641 aagagaatat aaagaatagt tctgtcccaa gaagaactct gaagatgatt cagccttctg
     8701 catctggatc tcttgttgga agagaaaatg agctgtccgc aggcttgtcc aaaaggaaac
     8761 atcggaatga ccacttaaca tctacaactt ccagccctgg ggttattgtc ccagaatcta
     8821 gtgaaaataa aaatcttgga ggagtcaccc aggagtcatt tgatcttatg attaaagaaa
     8881 atccatcctc tcagtattgg aaggaagtgg cagaaaaacg gagaaaggcg ctgtaagtcg
     8941 agctgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     9001 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     9061 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     9121 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggtcg agc
//
