LOCUS       sequence-236323-        4550 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly CD part for mfLon-pdt3 (mfLon protease 
            with self-targeting degradation tag pdt3). Please see Supplemental 
            Documents for annotated Genbank file..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4550)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 4550)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: C109m
FEATURES             Location/Qualifiers
     source          1..4550
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     38..56
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(60..103)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    106..127
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    2599..2619
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(2625..2642)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2796..3384)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2876..2895)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3555..4346,4347..4415))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4178..4197
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4416..4520)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggcagaa
       61 tttcagataa aaaaaatcct tagctttcgc taaggatgat ttctggaatt cgcggccgct
      121 tctagagact agtgggtctc aaatgtctaa aaaaatcaaa ctgccgatct tccagatccg
      181 tggttctttc atcgttccgg gtatcaaaga aaacctggaa gttggtcgta aaaacaccct
      241 ggcgtctgtt aactacgcga tcaaaaactc taacaaccag atgatcgcga tcccgcagat
      301 cgacgcgtct gttgaaaaac cggaattctc tgacctgcac gaattcggta tcctgatcga
      361 cttcgaagtt atcaaagaat ggaaagacaa ctctctgacc atctctacca acccgatcca
      421 gcgttgcaaa gttatctctt tcttcgaaaa cgaagatcag gttccgtacg cggaagttga
      481 actgatcgaa tctatcaacg acttctctga cgaagaactg aaagaactga tcgaaaaaat
      541 ctctgacgcg atcaaaacca aagcgtctct ggttaccaaa cagatcaaac agctgatctc
      601 tggtgaatct gacgacctgt ctctggcgtt cgactctatc atgttcaaac tggcgccgtc
      661 taaaatcctg accaacccgg aatacatcac ctctccgtct ctgaaaaccc gttggtctat
      721 catcgaaaaa atcatcttcg cggaagatgg tatcatcacc cgtaacgcgg aatctatcga
      781 cgcggcgcgt cagaaaaacg aaatcgaaca ggaactgaac cacaaactga aagaaaaaat
      841 ggacaaacag cagaaagaat actacctgcg tgaaaaaatg cgtatcatca aagacgaact
      901 ggaagatgaa gatgactctg acgactcttc tctggaaaaa tacaaagaac gtctggcgaa
      961 agaaccgttc ccggaagaag ttaaacgtaa aatcatggcg tctatcaaac gtgttgaagc
     1021 gctgcagtct ggtaccccgg aatggaacac cgaaaaaaac tacatcgact ggatgatgtc
     1081 tatcccgtgg tgggaagaaa ccgaagatct gaccgacctg aaatacgcga aaaaaatcct
     1141 ggacaaacac cactacggta tgaaaaaagt taaagaacgt atcatcgaat acctggcggt
     1201 taaaaccaaa accaaatctc tgaaagcgcc gatcatcacc ctggttggtc cgccgggtgt
     1261 tggtaaaacc tctctggcga aatctatcgc ggaagcggtt ggtaaaaact tcgttaaagt
     1321 ttctctgggt ggtgttaaag acgaatctga aatccgtggt caccgtaaaa cctacgttgg
     1381 ttctatgccg ggtcgtatca tccagaccat gaaacgtgcg aaagttaaaa acccgctgtt
     1441 cctgctggac gaaatcgaca aaatggcgtc tgaccaccgt ggtgacccgg cgtctgcgat
     1501 gctggaagtt ctggacccgg aacagaacaa agaattctct gaccactaca tcgaagaacc
     1561 gtacgacctg tctcaggtta tgttcatcgc gaccgcgaac tacccggaag atatcccgga
     1621 agcgctgtac gaccgtatgg aaatcatcaa cctgtcctct tacaccgaaa tcgaaaaagt
     1681 taaaatcgcg caggactacc tggttccgaa agcgatcgaa cagcacgaac tgacctctga
     1741 agaaatctct ttcaccgaag gtgcgatcaa cgaaatcatc aaatactaca cccgtgaagc
     1801 gggtgttcgt cagctggaac gtcacatcaa ctctatcatc cgtaaataca tcgttaaaaa
     1861 cctgaacggt gaaatggaca aaatcgttat cgacgaaaaa caggttaacg acctgctggg
     1921 taaacgtatc ttcgaccaca ccgaaaaaca ggaagaatct cagatcggtg ttgttaccgg
     1981 tctggcgtac acccagttcg gtggtgacat cctgccgatc gaagtttctc tgtacccggg
     2041 taaaggtaac ctgatcctga ccggtaaact gggtgaagtt atgaaagaat ctgcgaccat
     2101 cgcgctgacc tacgttaaat ctaacttcga aaaattcggt gttgacaaaa aagttttcga
     2161 agaaaacgac atccacgttc acgttccgga aggtgcggtt ccgaaagacg gtccgtctgc
     2221 gggtatcacc atcaccaccg cgctgatctc tgcgctgtct gacaaaccgg tttctaaaga
     2281 aatcggtatg accggtgaaa tcaccctgcg tggtaacgtt ctgccgatcg gtggtctgcg
     2341 tgaaaaatct atctctgcgt ctcgttctgg tctgaaaacc atcatcatcc cgaaaaaaaa
     2401 cgaacgtgac ctggacgaaa tcccggacga agttaaagcg aaactgaaaa tcatcccggc
     2461 ggaaaaatac gaagaagttt tcgcgatcgt tttcaaaacc aaagcggcga acaaaaacga
     2521 agaaaacacc aacgaagtgc cgacctttat gctgaacgcg ggccaggcga acagaagacg
     2581 agtttaaagg tagagaccta ctagtagcgg ccgctgcagg cttcctcgct cactgactcg
     2641 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     2701 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     2761 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc acaggctccg cccccctgac
     2821 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     2881 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     2941 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     3001 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     3061 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     3121 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     3181 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     3241 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     3301 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     3361 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     3421 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     3481 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     3541 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     3601 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     3661 ttaccatctg gccccagtgc tgcaatgata ccgcgcgacc cacgctcacc ggctccagat
     3721 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     3781 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     3841 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     3901 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     3961 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     4021 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     4081 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     4141 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     4201 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     4261 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     4321 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     4381 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     4441 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     4501 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
//
