LOCUS       sequence-236341-        8506 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human CYFIP2 in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8506)
  AUTHORS   Pathania M, Davenport EC, Muir J, Sheehan DF, Lopez-Domenech G, 
            Kittler JT
  TITLE     The autism and schizophrenia associated gene CYFIP1 is critical for 
            the maintenance of dendritic complexity and the stabilization of 
            mature spines.
  JOURNAL   Transl Psychiatry. 2014 Mar 25;4:e374. doi: 10.1038/tp.2014.16.
  PUBMED    24667445
REFERENCE   2  (bases 1 to 8506)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Transl 
            Psychiatry."; date: "2014-03-25"; volume: "4:e374. doi"; pages: " 
            10.1038/tp.2014.16"
COMMENT     Sequence Label: CYFIP2.mCherry
FEATURES             Location/Qualifiers
     source          1..8506
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      285..873
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     774..793
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        1048..1351
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1352..1555
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1506..1526
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    1589..1613
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5382..5406)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      5420..5429
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5426..6136
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5571..5589)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5839..5858
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    6281..6402
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6318..6337)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6372..6391
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(6409..6864)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6546..6567)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6758..6777
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6891..6995
                     /gene="bla"
                     /label=AmpR promoter
     promoter        6997..7354
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6997..7188
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7020..7040)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7205..7340
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7389..8183
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7389..8183
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7443..7462)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8053..8072
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8371..8390)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8415..8462
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc
       61 ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata
      121 gcctcaggtt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
      181 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
      241 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
      301 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
      361 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
      421 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
      481 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
      541 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
      601 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
      661 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
      721 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
      781 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
      841 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
      901 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
      961 gtggataacc gtattaccgc catgcattag ttattaatag taatcaatta cggggtcatt
     1021 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
     1081 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
     1141 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
     1201 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
     1261 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
     1321 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
     1381 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
     1441 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
     1501 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctggttt
     1561 agtgaaccgt cagatccgct agcatcaaac aagtttgtac aaaaaagcag gctccaccat
     1621 gaccacgcac gtcaccctgg aagatgccct gtccaacgtg gacctgcttg aagagcttcc
     1681 cctccccgac cagcagccat gcatcgagcc tccaccttcc tccatcatgt accaggctaa
     1741 ctttgacaca aactttgagg acaggaatgc atttgtcacg ggcattgcaa ggtacattga
     1801 gcaggctaca gtccactcca gcatgaatga gatgctggag gaaggacatg agtatgcggt
     1861 catgctgtac acctggcgca gctgttcccg ggccattccc caggtgaaat gcaacgagca
     1921 gcccaaccga gtagagatct atgagaagac agtagaggtg ctggagccgg aggtcaccaa
     1981 gctcatgaag ttcatgtatt ttcagcgcaa ggccatcgag cggttctgca gcgaggtgaa
     2041 gcggctgtgc catgccgagc gcaggaagga ctttgtctct gaggcctacc tcctgaccct
     2101 tggcaagttc atcaacatgt ttgctgtcct ggatgagcta aagaacatga agtgcagcgt
     2161 caagaatgac cactctgcct acaagagggc agcacagttc ctgcggaaga tggcagatcc
     2221 ccagtctatc caggagtcgc agaacctttc catgttcctg gccaaccaca acaggatcac
     2281 ccagtgtctc caccagcaac ttgaagtgat cccaggctat gaggagctgc tggctgacat
     2341 tgtcaacatc tgtgtggatt actacgagaa caagatgtac ctgactccca gtgagaaaca
     2401 tatgctcctc aaggtgatgg gctttggcct ctacctaatg gatggaaatg tcagtaacat
     2461 ttacaaactg gatgccaaga agagaattaa tcttagcaaa attgataaat tctttaagca
     2521 gctgcaggtg gtgccccttt tcggcgacat gcagatagag ctggccagat acattaagac
     2581 cagtgctcac tatgaagaga acaagtccaa gtggacgtgc acccagagca gcatcagccc
     2641 ccagtacaat atctgcgagc agatggttca gatccgggat gaccacatcc gcttcatctc
     2701 cgagctcgct cgctacagca acagtgaggt ggtgacgggc tcagggctgg acagccagaa
     2761 gtcagacgag gagtatcgcg agctcttcga cctagccctg cggggtctgc agcttctatc
     2821 caagtggagc gcccacgtca tggaggtgta ctcttggaag ctggttcatc ccacagacaa
     2881 gttctgcaac aaggactgtc ctggcaccgc ggaggaatat gagagagcca cacgctacaa
     2941 ttacaccagt gaggaaaaat ttgccttcgt tgaggtgatc gccatgatca aaggcctgca
     3001 ggtgctcatg ggcaggatgg agagcgtctt caaccaggcc atcaggaaca ccatctacgc
     3061 ggcattgcag gacttcgccc aggtgacgct gcgtgagccc ctgcggcagg cggtacggaa
     3121 gaagaagaat gtcctcatca gcgtcctaca ggcaattcga aagaccatct gtgactggga
     3181 gggagggcga gagcccccta atgacccatg cttgagaggg gagaaggacc ccaaaggtgg
     3241 atttgatatc aaggtgcccc ggcgtgctgt ggggccatcc agcacacagc tgtacatggt
     3301 gcggaccatg cttgaatcac tcattgcaga caaaagcggc tccaagaaga ccctgaggag
     3361 cagcctggat ggacccattg tcctcgccat agaggacttt cacaaacagt ccttcttctt
     3421 cacacatctg ctcaacatca gtgaagccct gcagcagtgt tgtgacctct cccagctctg
     3481 gttccgagaa ttcttcctgg agttaaccat gggccgacga atccagttcc ccatcgagat
     3541 gtccatgccc tggattctaa cggaccatat cctggaaacc aaagaacctt ccatgatgga
     3601 gtatgtcctc taccctctgg atctgtacaa cgacagcgcc tactatgctc tgaccaagtt
     3661 taaaaagcag ttcctgtacg atgagataga agctgaggtg aacctgtgtt ttgatcagtt
     3721 tgtctacaag ctggcagacc agatctttgc ttactacaaa gccatggctg gcagtgtcct
     3781 gttggataaa cgttttcgag ctgagtgtaa gaattatggc gtcatcattc cgtatccacc
     3841 gtccaatcgc tatgaaacac tgctgaagca gagacacgtc cagctgttgg gtagatcaat
     3901 tgacttgaac agactcatta cccagcgcac ctctgccgcc atgtataaat ccttggacca
     3961 agctatcagc cgctttgaga gtgaggacct gacctccatt gtggagctgg agtggctgct
     4021 ggagattaac cggctcacgc atcggctgct ctgtaagcat atgacgctgg acagcttcga
     4081 tgccatgttc cgagaggcca atcacaatgt gtccgccccc tatggccgta tcaccctgca
     4141 tgtcttctgg gaactgaact ttgactttct ccccaactac tgctacaatg ggtccactaa
     4201 ccgttttgtg cggactgcca ttcctttcac ccaagaacca caacgagaca aacctgccaa
     4261 cgtccagcct tattacctct atggatccaa gcctctcaac attgcctaca gccacatcta
     4321 cagctcctac aggaatttcg tggggccacc tcatttcaag actatctgca gactcctggg
     4381 ttatcagggc atcgctgtgg tcatggagga actgctaaag attgtgaaga gcttgctcca
     4441 aggaaccatt ctccagtatg tgaaaacact gatagaggtg atgcccaaga tatgccgctt
     4501 gccccgacat gagtatggct ccccagggat cctggagttc ttccaccacc agctgaagga
     4561 catcattgag tacgcagagc tcaaaacaga cgtgttccag agcctgaggg aagtgggcaa
     4621 tgccatcctc ttctgcctcc tcatagagca agctctgtct caggaggagg tctgcgattt
     4681 gctccatgcc gcacccttcc aaaacatctt gcctagagtc tacatcaaag agggggagcg
     4741 cctggaggtc cggatgaaac gtctggaagc caagtatgcc ccgctccacc tggtccctct
     4801 gatcgagcgg ctggggaccc ctcagcaaat cgccattgct cgcgagggtg acctcctgac
     4861 caaggagcgg ctgtgctgtg gcctgtccat gttcgaggtc atcctgaccc gcattcggag
     4921 ctacctgcag gaccccatct ggcggggccc accgcccacc aatggcgtca tgcacgtcga
     4981 tgagtgtgtg gagttccacc ggctgtggag cgccatgcag ttcgtgtact gcatccctgt
     5041 gggaaccaac gagttcacag ctgagcagtg tttcggcgat ggcttgaact gggctggttg
     5101 ctccatcatt gtcctgctgg gccagcagcg tcgctttgac ctgttcgact tctgttacca
     5161 cctgctaaaa gtgcagaggc aggacgggaa ggatgaaatc attaagaatg tgcccctgaa
     5221 gaagatggcc gaccggatca ggaagtatca gatcttgaac aatgaggttt ttgccatcct
     5281 gaacaaatac atgaagtccg tggagacaga cagttccact gtggagcatg tgcgctgctt
     5341 ccagccaccc atccaccagt ccttggccac cacttgcttg gacccagctt tcttgtacaa
     5401 agtggttcga tgggatcccg ccaccatggt gagcaagggc gaggaggata acatggccat
     5461 catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt
     5521 cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa
     5581 ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta
     5641 cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt
     5701 ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt
     5761 gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac
     5821 caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc
     5881 cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct
     5941 gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt
     6001 gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga
     6061 ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga
     6121 cgagctgtac aagtaagaat tcgaagctgt acaagtaaag cggccgcgac tctagatcat
     6181 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
     6241 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
     6301 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     6361 gcattctagt tgtggtttgt ccaaactcat caatgtatct taaggcgtaa attgtaagcg
     6421 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
     6481 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
     6541 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
     6601 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
     6661 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
     6721 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
     6781 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
     6841 ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     6901 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     6961 gataaatgct tcaataatat tgaaaaagga agagtcctga ggcggaaaga accagctgtg
     7021 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     7081 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     7141 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     7201 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     7261 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     7321 aggaggcttt tttggaggcc taggcttttg caaagatcga tcaagagaca ggatgaggat
     7381 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
     7441 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     7501 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     7561 atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     7621 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     7681 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
     7741 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     7801 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     7861 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca
     7921 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
     7981 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     8041 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     8101 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     8161 gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac
     8221 gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
     8281 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
     8341 gttcttcgcc caccctaggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg
     8401 aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt
     8461 tcataaacgc ggggttcggt cccagggctg gcactctgtc gatacc
//
