LOCUS       sequence-236342-        2165 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly DE part for ECK120033737 (313x-strength 
            natural terminator; see DOI: 10.1038/nmeth.2515). Please see 
            Supplemental Documents for annotated Genbank file..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2165)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: T13m
FEATURES             Location/Qualifiers
     source          1..2165
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     118..135
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    141..161
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     misc_feature    248..269
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      272..315
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(319..337)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        405..509
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(510..578,579..1370)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(728..747)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1541..2129
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2030..2049
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
       61 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
      121 gagtcagtga gcgaggaagc ctgcagcggc cgctactagt aggtctctaa gccctttggt
      181 cgaaaaaaaa agcccgcact gtcaggtgcg ggcttttttc tgtgtttcca ccttgagacc
      241 cactagtctc tagaagcggc cgcgaattcc agaaatcatc cttagcgaaa gctaaggatt
      301 ttttttatct gaaattctgc ctcgtgatac gcctattttt ataggttaat gtcatgataa
      361 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
      421 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
      481 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
      541 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
      601 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
      661 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
      721 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
      781 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
      841 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
      901 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
      961 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     1021 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     1081 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     1141 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     1201 ataaatctgg agccggtgag cgtgggtcgc gcggtatcat tgcagcactg gggccagatg
     1261 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     1321 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     1381 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     1441 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     1501 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     1561 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     1621 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     1681 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     1741 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     1801 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     1861 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     1921 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     1981 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     2041 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     2101 gctcgtcagg ggggcggagc ctgtggaaaa acgccagcaa cgcggccttt ttacggttcc
     2161 tggcc
//
