LOCUS       sequence-236346-        8506 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human CYFIP1 in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8506)
  AUTHORS   Pathania M, Davenport EC, Muir J, Sheehan DF, Lopez-Domenech G, 
            Kittler JT
  TITLE     The autism and schizophrenia associated gene CYFIP1 is critical for 
            the maintenance of dendritic complexity and the stabilization of 
            mature spines.
  JOURNAL   Transl Psychiatry. 2014 Mar 25;4:e374. doi: 10.1038/tp.2014.16.
  PUBMED    24667445
REFERENCE   2  (bases 1 to 8506)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Transl 
            Psychiatry."; date: "2014-03-25"; volume: "4:e374. doi"; pages: " 
            10.1038/tp.2014.16"
COMMENT     Sequence Label: CYFIP1.mCherry
FEATURES             Location/Qualifiers
     source          1..8506
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    602..626
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      630..639
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(4395..4419)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      4433..4442
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4439..5149
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4584..4602)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4852..4871
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    5294..5415
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5331..5350)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5385..5404
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(5422..5877)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5559..5580)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5771..5790
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5904..6008
                     /gene="bla"
                     /label=AmpR promoter
     promoter        6010..6367
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6010..6201
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6033..6053)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6218..6353
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6402..7196
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6402..7196
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6456..6475)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7066..7085
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(7384..7403)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    7428..7475
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7804..8392
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8293..8312
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcatca
      601 aacaagtttg tacaaaaaag caggctccac catggcggcc caggtgactc tggaggacgc
      661 gctgtccaac gtggacctcc tggaggagct gcccctgccc gaccagcagc cctgcatcga
      721 gcccccgcca tcctcgctgc tctaccagcc aaatttcaac actaactttg aagacagaaa
      781 tgcatttgtt actggcatcg caagatacat tgaacaagcc accgtccact ctagcatgaa
      841 cgagatgctg gaggagggcc aagaatatgc tgtcatgctg tacacctgga ggagctgctc
      901 ccgggccatc ccacaggtga aatgtaacga gcagcctaac agagtggaaa tctacgagaa
      961 aaccgtggag gttctggagc ctgaggtcac aaaactgatg aatttcatgt acttccagag
     1021 aaatgccatt gagcgtttct gcggggaagt gaggcgcctg tgccatgccg agaggaggaa
     1081 ggacttcgtg tcagaagcct acctgatcac actgggcaaa ttcatcaaca tgttcgctgt
     1141 gctggacgag ctgaagaaca tgaagtgcag tgtgaagaac gaccactcag cgtacaagag
     1201 ggccgctcag tttttacgta aaatggcaga tccacagtcc atccaggaat cgcagaatct
     1261 gtccatgttc ctggccaatc ataacaagat cacacagtct ctgcagcagc agctcgaagt
     1321 gatttctggc tacgaagagc tcctggcaga tattgtgaat ctgtgtgtgg attactacga
     1381 gaacaggatg tatttgacgc ccagtgagaa acacatgctt ctcaaagtca tgggatttgg
     1441 tctgtacctg atggatggga gtgtcagtaa catctataag ttggatgcca agaaaagaat
     1501 aaacttatcc aaaatcgaca agtacttcaa gcaactccag gtggttccac tatttgggga
     1561 catgcaaata gaactggcaa gatatatcaa gaccagcgcc cactacgagg aaaataaatc
     1621 tcgatggacg tgcacatcct ccggcagcag ccctcagtac aacatctgcg agcagatgat
     1681 ccagatccgc gaggaccaca tgcgcttcat ttcggagctg gcgcgctaca gcaacagcga
     1741 ggtggtcacg ggctcgggcc gccaggaggc ccagaagacg gacgcggagt accgcaagct
     1801 cttcgacctg gcgctgcagg gcctgcagct gttgtcgcag tggagcgcgc acgtgatgga
     1861 agtgtattcc tggaagcttg tgcaccccac cgacaagtac tccaacaagg actgccccga
     1921 cagcgctgaa gagtacgagc gtgccacgcg ctacaactac accagcgagg agaagtttgc
     1981 cctagtggag gtgatcgcca tgatcaaagg cctgcaggtg ctgatgggca ggatggagag
     2041 cgtgttcaac cacgccatcc ggcacaccgt ctatgccgca ctgcaggact tctcccaggt
     2101 gacccttagg gagccgctgc ggcaggccat caagaagaag aagaacgtca tccagagtgt
     2161 cctgcaggcc atcaggaaga ccgtgtgtga ctgggagacg gggcatgagc ccttcaatga
     2221 cccagccttg cggggcgaga aggaccccaa gagcggcttc gacataaaag taccacgccg
     2281 cgccgtggga ccctccagca ctcagcttta catggtgaga accatgctag agtccctcat
     2341 tgcagacaaa agtggttcca agaaaacctt gagaagtagc cttgaggggc ccaccatatt
     2401 ggacatagaa aaatttcatc gagagtcatt cttctacact cacttgataa atttcagtga
     2461 aacgctgcag cagtgctgtg acctttcgca gctgtggttc cgagagttct tcctggagct
     2521 gaccatgggc aggaggatcc agttccccat tgagatgtcg atgccctgga tcctgacgga
     2581 ccacatcctg gagaccaagg aggcatcgat gatggagtac gtgctctact ccctggacct
     2641 gtacaatgac agcgcccact acgcgctcac caggttcaac aagcagttcc tgtacgacga
     2701 aattgaggcc gaggtgaatc tatgttttga ccaatttgtt tacaagctag cagaccagat
     2761 atttgcctat tataaggtta tggcaggaag tttgcttctt gataaacggt tacgatcaga
     2821 atgcaagaat cagggagcca cgatccacct cccgccgtct aaccgctacg agacgctgct
     2881 gaagcagagg catgtgcagc tcctcggcag atcaatagac ctcaatcgtc tgatcaccca
     2941 gcgcgtctca gcagccatgt ataagtccct agaactggcg attggacgat ttgaaagtga
     3001 agatttgacc tccatagttg agctggatgg cctgttggaa atcaaccgca tgacccacaa
     3061 gctgctgagc cggtacctga cgctggacgg cttcgacgcc atgttccggg aggccaacca
     3121 caacgtgtca gcgccctacg ggaggatcac cctgcacgtc ttctgggagc tcaactatga
     3181 cttcctgccc aactactgct acaacggctc taccaaccgg tttgttcgga cagtgttacc
     3241 attttctcag gaatttcaaa gagataagca gcctaatgca cagcctcagt atctgcatgg
     3301 atccaaggct ttgaacttgg cctactccag catttacggc agctaccgga acttcgtggg
     3361 acctccacac tttcaagtca tctgccggct tctcggctac cagggtatcg ccgtggtcat
     3421 ggaggagctg ctgaaggtcg tcaagagcct gctgcaaggc acaatcctgc agtacgtgaa
     3481 gacgctgatg gaggtgatgc ccaagatctg ccgcctgccc cggcacgagt acggctctcc
     3541 tggtatcctg gagttcttcc accaccagct gaaggacatc gtggagtacg cagagctgaa
     3601 gacggtgtgc ttccagaacc tgcgggaggt ggggaacgcc atcctcttct gcctgctcat
     3661 cgagcagagc ctgtctttag aagaagtgtg tgacctgctg cacgcggctc ctttccagaa
     3721 catcttgccg cgagtccatg tgaaagaggg ggagagactt gatgccaaaa tgaaaagact
     3781 agaatcaaag tacgccccgc tgcatcttgt cccactgatt gaaagactgg ggacccctca
     3841 gcaaattgcc atcgcaagag agggggacct gctgacaaag gagcgcctct gctgcggcct
     3901 gtccatgttt gaggtcatcc tgacacggat ccggagcttt ctggatgacc ccatctggcg
     3961 cgggcctctg cccagcaatg gggtcatgca tgtggacgag tgtgtggagt ttcacagact
     4021 gtggagtgcc atgcagtttg tctactgcat tcccgtgggg acacacgagt tcacagtcga
     4081 gcagtgcttt ggtgatgggc tacactgggc tggctgtatg atcatcgtac ttcttgggca
     4141 gcagcggcgt tttgctgtgc tggatttctg ctaccatcta cttaaagtcc agaaacatga
     4201 tggcaaagat gagattatta aaaatgtgcc tttgaagaag atggtggaga gaattcgcaa
     4261 gttccagatt ctcaatgatg agatcatcac catcctggat aagtacctga agtcaggcga
     4321 cggggagggc acgccagtgg agcatgtgcg ctgcttccag ccgcccatcc accagtccct
     4381 cgccagcagc ttggacccag ctttcttgta caaagtggtt cgatgggatc ccgccaccat
     4441 ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt
     4501 gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg
     4561 cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt
     4621 cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc
     4681 cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt
     4741 gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg
     4801 cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat
     4861 gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc
     4921 cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga
     4981 ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa
     5041 catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg
     5101 cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagtaag aattcgaagc
     5161 tgtacaagta aagcggccgc gactctagat cataatcagc cataccacat ttgtagaggt
     5221 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc
     5281 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     5341 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     5401 catcaatgta tcttaaggcg taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
     5461 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     5521 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     5581 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     5641 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
     5701 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
     5761 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
     5821 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcag
     5881 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     5941 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     6001 ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa
     6061 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     6121 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     6181 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     6241 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     6301 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     6361 ttgcaaagat cgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat
     6421 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
     6481 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
     6541 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc
     6601 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
     6661 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
     6721 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
     6781 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
     6841 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
     6901 cagccgaact gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga
     6961 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
     7021 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
     7081 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
     7141 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg
     7201 gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga
     7261 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
     7321 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccta gggggaggct
     7381 aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag
     7441 acagaataaa acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg
     7501 ctggcactct gtcgataccc caccgagacc ccattggggc caatacgccc gcgtttcttc
     7561 cttttcccca ccccaccccc caagttcggg tgaaggccca gggctcgcag ccaacgtcgg
     7621 ggcggcaggc cctgccatag cctcaggtta ctcatatata ctttagattg atttaaaact
     7681 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     7741 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     7801 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     7861 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     7921 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     7981 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     8041 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     8101 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     8161 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     8221 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     8281 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     8341 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     8401 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     8461 tgcgttatcc cctgattctg tggataaccg tattaccgcc atgcat
//
