LOCUS       sequence-236356-        2159 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly DE part for L3S1P13 (178x-strength 
            synthetic terminator; see DOI: 10.1038/nmeth.2515). Please see 
            Supplemental Documents for annotated Genbank file..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2159)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: T14m
FEATURES             Location/Qualifiers
     source          1..2159
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..64
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(68..111)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    114..135
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    216..236
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(242..259)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(413..1001)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(493..512)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1172..1963,1964..2032))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1795..1814
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2033..2137)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
       61 aggcagaatt tcagataaaa aaaatcctta gctttcgcta aggatgattt ctggaattcg
      121 cggccgcttc tagagactag tgggtctcaa ggtgacgaac aataaggcct ccctaacggg
      181 gggccttttt tattgataac aaaagcttag agacctacta gtagcggccg ctgcaggctt
      241 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
      301 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
      361 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccaca
      421 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
      481 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
      541 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
      601 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
      661 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
      721 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
      781 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
      841 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
      901 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
      961 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     1021 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     1081 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     1141 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     1201 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     1261 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgcgacccac
     1321 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     1381 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     1441 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     1501 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     1561 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     1621 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     1681 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     1741 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     1801 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     1861 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     1921 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     1981 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     2041 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     2101 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgcca
//
