LOCUS       sequence-236362-        8470 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Produces equimolar expression of eCFP and mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8470)
  AUTHORS   Botman D, de Groot DH, Schmidt P, Goedhart J, Teusink B
  TITLE     In vivo characterisation of fluorescent proteins in budding yeast.
  JOURNAL   Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.1038/s41598-019-38913-z.
  PUBMED    30783202
REFERENCE   2  (bases 1 to 8470)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2019-02-19"; volume: "9(1):2234. doi"; pages: " 
            10.1038/s41598-019-38913-z"
COMMENT     Sequence Label: pDRF1-GW eCFP-T2A-mCherry
FEATURES             Location/Qualifiers
     source          1..8470
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    800..824
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      836..845
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(842..844,845..847,848..1558)
                     /codon_start=1
                     /product="cyan variant of GFP"
                     /label=CFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(887..908)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1148..1167)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1495..1516
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1565..1618
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      1634..1643
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1640..2350
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1785..1803)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2053..2072
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(2360..2384)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(2446..2465)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2447..2465)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      2613..2800
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2668..2692)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(2812..2829)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2812..2828)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2821..2843)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3264..4610
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(4721..5524)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(5525..5740)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(5765..5783)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5851..5955
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5956..6024,6025..6816)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6174..6193)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6987..7575
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7476..7495
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7729..7746
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7863..7884
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7899..7904,7905..7922,7923..7929)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7937..7953
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7942..7964
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7961..7977
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7961..7977
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(8007..8462)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8144..8165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8356..8375
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 caagcttcct gaaacggaga aacataaaca ggcattgctg ggatcaccca tacatcactc
       61 tgttttgcct gaccttttcc ggtaatttga aaacaaaccc ggtctcgaag cggagatccg
      121 gcgataatta ccgcagaaat aaacccatac acgagacgta gaaccagccg cacatggccg
      181 gagaaactcc tgcgagaatt tcgtaaactc gcgcgcattg catctgtatt tcctaatgcg
      241 gcacttccag gcctcgatcg agaccgttta tccattgctt ttttgttgtc tttttccctc
      301 gttcacagaa agtctgaaga agctatagta gaactatgag ctttttttgt ttctgttttc
      361 cttttttttt tttttacctc tgtggaaatt gttactctca cactctttag ttcgtttgtt
      421 tgttttgttt attccaatta tgaccggtga cgaaacgtgg tcgatggtgg gtaccgctta
      481 tgctcccctc cattagtttc gattatataa aaaggccaaa tattgtatta ttttcaaatg
      541 tcctatcatt atcgtctaac atctaatttc tcttaaattt tttctctttc tttcctataa
      601 caccaatagt gaaaatcttt ttttcttcta tatctacaaa aacttttttt ttctatcaac
      661 ctcgttgata aattttttct ttaacaatcg ttaataatta attaattgga aaataaccat
      721 tttttctctc ttttatacac acattcaaaa gaaagaaaaa aaatataccc cagcctcgac
      781 tagtgaattc gcggccatca caagtttgta caaaaaagca ggctgctagc cggtcgccac
      841 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
      901 cggcgacgta aacggccaca ggttcagcgt gtccggcgag ggcgagggcg atgccaccta
      961 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     1021 cctcgtgacc accctgacct ggggcgtgca gtgcttcagc cgctaccccg accacatgaa
     1081 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gtaccatctt
     1141 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     1201 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     1261 caagctggag tacaactaca tcagccacaa cgtctatatc accgccgaca agcagaagaa
     1321 cggcatcaag gcccacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
     1381 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     1441 ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
     1501 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagtc
     1561 cggagagggc agaggaagtc ttctaacatg cggtgacgtg gaggagaatc ccggccctgg
     1621 ggatccaccg gtcgccacca tggtgagcaa gggcgaggag gataacatgg ccatcatcaa
     1681 ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat
     1741 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac
     1801 caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc
     1861 caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga
     1921 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca
     1981 ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt
     2041 cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg
     2101 gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga
     2161 cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct
     2221 gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac
     2281 catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct
     2341 gtacaagtaa agcggccgca cccagctttc ttgtacaaag tggtgtagtt aattcatgat
     2401 ggccgctgca ggtcgacctc gagggggggc ccggtaccca attcgcccta tagtgagtcg
     2461 tattacgcgc ggatccagct ttggacttct tcgccagagg tttggtcaag tctccaatca
     2521 aggttgtcgg cttgtctacc ttgccaaaaa tttacgaaaa gatggaaaag ggtcaaatcg
     2581 ttggtagata cgttgttgac acttctaaat aagcgaattt cttatgattt atgattttta
     2641 ttattaaata agttataaaa aaaataagtg tatacaaatt ttaaagtgac tcttaggttt
     2701 taaaacgaaa attcttattc ttgagtaact ctttcctgta ggtcaggttg ctttctcagg
     2761 tatagcatga ggtcgctctt attgaccaca cctctaccgg catgccaatt cactggccgt
     2821 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     2881 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     2941 acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg tattttctcc ttacgcatct
     3001 gtgcggtatt tcacaccgca taatcggatc gtacttgtta cccatcattg aattttgaac
     3061 atccgaacct gggagttttc cctgaaacag atagtatatt tgaacctgta taataatata
     3121 tagtctagcg ctttacggaa gacaatgtat gtatttcggt tcctggagaa actattgcat
     3181 ctattgcata ggtaatcttg cacgtcgcat ccccggttca ttttctgcgt ttccatcttg
     3241 cacttcaata gcatatcttt gttaacgaag catctgtgct tcattttgta gaacaaaaat
     3301 gcaacgcgag agcgctaatt tttcaaacaa agaatctgag ctgcattttt acagaacaga
     3361 aatgcaacgc gaaagcgcta ttttaccaac gaagaatctg tgcttcattt ttgtaaaaca
     3421 aaaatgcaac gcgagagcgc taatttttca aacaaagaat ctgagctgca tttttacaga
     3481 acagaaatgc aacgcgagag cgctatttta ccaacaaaga atctatactt cttttttgtt
     3541 ctacaaaaat gcatcccgag agcgctattt ttctaacaaa gcatcttaga ttactttttt
     3601 tctcctttgt gcgctctata atgcagtctc ttgataactt tttgcactgt aggtccgtta
     3661 aggttagaag aaggctactt tggtgtctat tttctcttcc ataaaaaaag cctgactcca
     3721 cttcccgcgt ttactgatta ctagcgaagc tgcgggtgca ttttttcaag ataaaggcat
     3781 ccccgattat attctatacc gatgtggatt gcgcatactt tgtgaacaga aagtgatagc
     3841 gttgatgatt cttcattggt cagaaaatta tgaacggttt cttctatttt gtctctatat
     3901 actacgtata ggaaatgttt acattttcgt attgttttcg attcactcta tgaatagttc
     3961 ttactacaat ttttttgtct aaagagtaat actagagata aacataaaaa atgtagaggt
     4021 cgagtttaga tgcaagttca aggagcgaaa ggtggatggg taggttatat agggatatag
     4081 cacagagata tatagcaaag agatactttt gagcaatgtt tgtggaagcg gtattcgcaa
     4141 tattttagta gctcgttaca gtccggtgcg tttttggttt tttgaaagtg cgtcttcaga
     4201 gcgcttttgg ttttcaaaag cgctctgaag ttcctatact ttctagctag agaataggaa
     4261 cttcggaata ggaacttcaa agcgtttccg aaaacgagcg cttccgaaaa tgcaacgcga
     4321 gctgcgcaca tacagctcac tgttcacgtc gcacctatat ctgcgtgttg cctgtatata
     4381 tatatacatg agaagaacgg catagtgcgt gtttatgctt aaatgcgtac ttatatgcgt
     4441 ctatttatgt aggatgaaag gtagtctagt acctcctgtg atattatccc attccatgcg
     4501 gggtatcgta tgcttccttc agcactaccc tttagctgtt ctatatgctg ccactcctca
     4561 attggattag tctcatcctt caatgctatc atttcctttg atattggatc gatccgatga
     4621 taagctgtca aacatgagaa ttgggtaata actgatataa ttaaattgaa gctctaattt
     4681 gtgagtttag tatacatgca tttacttata atacagtttt ttagttttgc tggccgcatc
     4741 ttctcaaata tgcttcccag cctgcttttc tgtaacgttc accctctacc ttagcatccc
     4801 ttccctttgc aaatagtcct cttccaacaa taataatgtc agatcctgta gagaccacat
     4861 catccacggt tctatactgt tgacccaatg cgtctccctt gtcatctaaa cccacaccgg
     4921 gtgtcataat caaccaatcg taaccttcat ctcttccacc catgtctctt tgagcaataa
     4981 agccgataac aaaatctttg tcgctcttcg caatgtcaac agtaccctta gtatattctc
     5041 cagtagatag ggagcccttg catgacaatt ctgctaacat caaaaggcct ctaggttcct
     5101 ttgttacttc ttctgccgcc tgcttcaaac cgctaacaat acctgggccc accacaccgt
     5161 gtgcattcgt aatgtctgcc cattctgcta ttctgtatac acccgcagag tactgcaatt
     5221 tgactgtatt accaatgtca gcaaattttc tgtcttcgaa gagtaaaaaa ttgtacttgg
     5281 cggataatgc ctttagcggc ttaactgtgc cctccatgga aaaatcagtc aagatatcca
     5341 catgtgtttt tagtaaacaa attttgggac ctaatgcttc aactaactcc agtaattcct
     5401 tggtggtacg aacatccaat gaagcacaca agtttgtttg cttttcgtgc atgatattaa
     5461 atagcttggc agcaacagga ctaggatgag tagcagcacg ttccttatat gtagctttcg
     5521 acatgattta tcttcgtttc ctgcatgttt ttgttctgtg cagttgggtt aagaatactg
     5581 ggcaatttca tgtttcttca acactacata tgcgtatata taccaatcta agtctgtgct
     5641 ccttccttcg ttcttccttc tgttcggaga ttaccgaatc aaaaaaattt caaggaaacc
     5701 gaaatcaaaa aaaagaataa aaaaaaaatg atgaattgaa aagctaattc ttgaagacga
     5761 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     5821 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     5881 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     5941 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     6001 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     6061 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     6121 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     6181 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     6241 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     6301 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     6361 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     6421 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     6481 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     6541 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     6601 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     6661 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     6721 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     6781 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     6841 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     6901 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     6961 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     7021 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     7081 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     7141 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     7201 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     7261 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     7321 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     7381 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     7441 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     7501 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     7561 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     7621 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     7681 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     7741 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     7801 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     7861 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
     7921 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
     7981 gattacgcca agcttaccgc atcaggaaat tgtaagcgtt aatattttgt taaaattcgc
     8041 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
     8101 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag
     8161 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
     8221 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc
     8281 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
     8341 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
     8401 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc
     8461 gtccattcgc
//
