LOCUS       sequence-236364-        1837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Homology repair template for in frame (first exon) clover knock-in 
            of human LMNA gene .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Pinder J, Salsman J, Dellaire G
  TITLE     Nuclear domain 'knock-in' screen for the evaluation and 
            identification of small molecule enhancers of CRISPR-based genome 
            editing.
  JOURNAL   Nucleic Acids Res. 2015 Oct 30;43(19):9379-92. doi: 
            10.1093/nar/gkv993. Epub 2015 Oct 1.
  PUBMED    26429972
REFERENCE   2  (bases 1 to 1837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gkv993"; journalName: "Nucleic Acids Res"; date: 
            "2015-10-30- 30"; volume: "43"; issue: "19"; pages: "9379-92"
COMMENT     Sequence Label: pCR2.1 Clover-LMNA Donor
FEATURES             Location/Qualifiers
     source          1..1837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(454..456,457..459,460..462,463..1173)
                     /codon_start=1
                     /product="bright green-yellow fluorescent protein derived 
                     from GFP (Lam et al., 2012)"
                     /label=Clover
                     /translation="MEVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLT
                     LKFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKD
                     DGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGI
                     KANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSALSKDPNEKRDHMVL
                     LEFVTAAGITHGMDELYK"
     primer_bind     complement(502..523)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     1110..1131
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ggcaagcttg gagccgacag tgctgagcag gcaggagcca agagagggga agcttgagcc
       61 tcacgcagtt aggggtgcgc tggagagggt ggggcccgac tccgccacac cccaacggtc
      121 cttccccctc ctcaccactc ccgcccccac ccccaatgga tctgggactg cccctttaag
      181 agtagtggcc cctcctccct tcagaggagg acctattaga gcctttgccc cggcgtcggt
      241 gactcagtgt tcgcgggagc gccgcaccta caccagccaa cccagatccc gaggtccgac
      301 agcgcccggc ccagatcccc acgcctgcca ggagcaagcc gagagccagc cggccggcgc
      361 actccgactc cgagcagtct ctgtccttcg acccgagccc cgcgcccttt ccgggacccc
      421 tgccccgcgg gcagcgctgc caacctgccg gccatggagg tgagcaaggg cgaggagctg
      481 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
      541 agcgtccgcg gcgagggcga gggcgatgcc accaacggca agctgaccct gaagttcatc
      601 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt cggctacggc
      661 gtggcctgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
      721 atgcccgaag gctacgtcca ggagcgcacc atctctttca aggacgacgg tacctacaag
      781 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
      841 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa cttcaacagc
      901 cacaacgtct atatcacggc cgacaagcag aagaacggca tcaaggctaa cttcaagatc
      961 cgccacaacg ttgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
     1021 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagccatca gtccgccctg
     1081 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     1141 gggattacac atggcatgga cgagctgtac aagaccccgt cccagcggcg cgccacccgc
     1201 agcggggcgc aggccagctc cactccgctg tcgcccaccc gcatcacccg gctgcaggag
     1261 aaggaggacc tgcaggagct caatgatcgc ttggcggtct acatcgaccg tgtgcgctcg
     1321 ctggaaacgg agaacgcagg gctgcgcctt cgcatcaccg agtctgaaga ggtggtcagc
     1381 cgcgaggtgt ccggcatcaa ggccgcctac gaggccgagc tcggggatgc ccgcaagacc
     1441 cttgactcag tagccaagga gcgcgcccgc ctgcagctgg agctgagcaa agtgcgtgag
     1501 gagtttaagg agctgaaagc gcggtgagtt cgcccaggtg gctgcgtgcc tggcggggag
     1561 tggagagggc ggcgggccgg cgcccctggc cggccgcagg aagggagtga gagggcctgg
     1621 aggccgataa ctttgccata gtctcctccc tccccggaac tgcccccagc gggtgactgg
     1681 cagtgtcaag gggaattgtc aagacaggac agagagggaa gtggtggtct ctgggagagg
     1741 gtcggggagg atataaggaa tggtgggggt atcagggaca agttggggct ggggccggcc
     1801 tgaattcggt cagattggga tttgccaact atttgga
//
