LOCUS       sequence-236366-        3401 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive integrase cassette - NO integrases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 546 to 580)
  AUTHORS   Guiziou S, Mayonove P, Bonnet J
  TITLE     Hierarchical composition of reliable recombinase logic devices.
  JOURNAL   Nat Commun. 2019 Jan 28;10(1):456. doi: 10.1038/s41467-019-08391-y.
  PUBMED    30692530
REFERENCE   2  (bases 1 to 3401)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-01-28"; volume: "10(1):456. doi"; pages: " 
            10.1038/s41467-019-08391-y"
COMMENT     Sequence Label: SG-C-Int-0
FEATURES             Location/Qualifiers
     source          1..3401
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             117..128
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      183..254
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      270..297
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        546..580
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     CDS             644..655
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(1097..1114)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1404..1423)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2019..2113)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             join(2211..2279,2280..3071)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3075..3177)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(3354..3397)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 gatactgtta cttaccgatt attgtgaaga accagaccgt ttgttgtagc acactcggcg
       61 aaaatctgaa tagtaacttc ttgacaatta atcatccggc tcgtaattta tgtggaaaag
      121 aggagaaata ctagataaag ttgtgccgta tccagcggtt accaataata gtctccaggc
      181 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
      241 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
      301 atatagtaaa gttccaataa gactccaggt atctgtccgt gtaaaaaaga gtattgactt
      361 cgcatctttt tgtacctata atgtgtggat cacacaggaa agtactagat tgaacctcta
      421 ctacgagtga gttgagatta cagccttact cggtaccaaa ttccagaaaa gagacgcttt
      481 cgagcgtctt ttttcgtttt ggtcccttac gctattattg aagccagtct gttaccgaag
      541 tgaaattgac ggctagctca gtcctaggta cagtgctagc cagaggaagg aggctcgaac
      601 gattacggat tgctctactg tgactgaagt ttacaaccca attattgaag gccgctaacg
      661 cggccttttt ttgtttctgg tctccctgag gcacagagat ttactttatt cacgacttca
      721 gatacgcaca gctaacacca cgtcgtccct atctgctgcc ctaggtctat gagtggttgc
      781 tggataactt tacgggcatg cataaggctc gtatgatata ttcagggaga ccacaacggt
      841 ttccctctac aaataatttt gtttaacttt agtaatttca acaaaataac taggattcga
      901 aatacggtct actacagaag ggtggtttat cttactcaac gacgaacaat aaggcctccc
      961 taacgggggg ccttttttat tgataacaaa aagatttccc tcgtcacgca gtaagtattt
     1021 atcgtagaag atcactttta tcggtttcca gaacaggtaa gagccaatag tgattacttc
     1081 gcgttatgca ggcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     1141 cggtatcagc tcactcaaag gcggtaatcc tagctagggc gttcggctgc ggcgagcggt
     1201 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     1261 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     1321 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     1381 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     1441 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     1501 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     1561 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     1621 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg ccacgactta
     1681 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     1741 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     1801 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     1861 caaaccaccg ctggtagcgg tggttttttg tttgcaagca gcagattacg cgcagaaaaa
     1921 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     1981 actcacgtta agggattttg gtcatgactt gtgcttggat tctcaccaat aaaaaacgcc
     2041 cggcggcaac cgagcgttct gaacaaatcc agatggagtt ctgaggtcat tactggatct
     2101 atcagcagga gtccaagcga gctcgatatc actaaataca ttcaaatatc tatccgctca
     2161 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagaat atgagtattc
     2221 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     2281 acccagaaac gctggtgaaa gtaaaagatg ccgaagatca gttgggtgca cgtgtgggtt
     2341 acatcgaact ggacctcaac agcggtaaga ttcttgagag ttttcgcccc gaagaacgtt
     2401 tcccaatgat gagcactttt aaagttctgc tctgtggcgc ggtattatcc cgtattgacg
     2461 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     2521 caccagtcac agaaaagcat cttacggacg gcatgacagt acgcgaatta tgcagcgctg
     2581 ccataaccat gagtgataac acggcggcca acttacttct gacaacgatc ggaggaccga
     2641 aggagcttac cgcttttttg cacaacatgg gtgatcatgt aactcgcctt gatcgttggg
     2701 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcta
     2761 tggcaacaac gttgcgcaaa ctcttaactg gcgaacttct tactctcgct tcccggcaac
     2821 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     2881 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     2941 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     3001 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tccctgatta
     3061 agcattggta ataatttagc ttccttagct cctgaaaatc tcgataactc aaaaaatacg
     3121 cccggtagtg atcttatttc attatggtga aagttggaac ctcttacgtg ccgatcaacg
     3181 tctcattttc gccagatatc gacgtctaag aaaccattat tatcatgaca ttaacctata
     3241 aaaataggcg tatcacgagg ccctttcgtc ttcacctcga gtgccacctg acgtctaaga
     3301 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc agaatttcag
     3361 ataaaaaaaa tccttagctt tcgctaagga tgatttctgg a
//
