LOCUS       sequence-236367-        8475 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Produces equimolar expression of mCherry and mTq2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8475)
  AUTHORS   Botman D, de Groot DH, Schmidt P, Goedhart J, Teusink B
  TITLE     In vivo characterisation of fluorescent proteins in budding yeast.
  JOURNAL   Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.1038/s41598-019-38913-z.
  PUBMED    30783202
REFERENCE   2  (bases 1 to 8475)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2019-02-19"; volume: "9(1):2234. doi"; pages: " 
            10.1038/s41598-019-38913-z"
COMMENT     Sequence Label: pDRF1-GW mCherry-T2A-mTurquoise2
FEATURES             Location/Qualifiers
     source          1..8475
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    800..824
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      841..850
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             847..1554
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(992..1010)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1260..1279
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             1561..1614
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      1630..1639
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1636..1638,1639..1641,1642..2355)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1681..1702)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1942..1961)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2289..2310
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(2365..2389)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(2451..2470)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2452..2470)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      2618..2805
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2673..2697)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(2817..2834)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2817..2833)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2826..2848)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3269..4615
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(4726..5529)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(5530..5745)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(5770..5788)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5856..5960
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5961..6029,6030..6821)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6179..6198)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6992..7580
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7481..7500
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7734..7751
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7868..7889
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7904..7909,7910..7927,7928..7934)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7942..7958
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7947..7969
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7966..7982
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7966..7982
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(8012..8467)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8149..8170)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8361..8380
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 caagcttcct gaaacggaga aacataaaca ggcattgctg ggatcaccca tacatcactc
       61 tgttttgcct gaccttttcc ggtaatttga aaacaaaccc ggtctcgaag cggagatccg
      121 gcgataatta ccgcagaaat aaacccatac acgagacgta gaaccagccg cacatggccg
      181 gagaaactcc tgcgagaatt tcgtaaactc gcgcgcattg catctgtatt tcctaatgcg
      241 gcacttccag gcctcgatcg agaccgttta tccattgctt ttttgttgtc tttttccctc
      301 gttcacagaa agtctgaaga agctatagta gaactatgag ctttttttgt ttctgttttc
      361 cttttttttt tttttacctc tgtggaaatt gttactctca cactctttag ttcgtttgtt
      421 tgttttgttt attccaatta tgaccggtga cgaaacgtgg tcgatggtgg gtaccgctta
      481 tgctcccctc cattagtttc gattatataa aaaggccaaa tattgtatta ttttcaaatg
      541 tcctatcatt atcgtctaac atctaatttc tcttaaattt tttctctttc tttcctataa
      601 caccaatagt gaaaatcttt ttttcttcta tatctacaaa aacttttttt ttctatcaac
      661 ctcgttgata aattttttct ttaacaatcg ttaataatta attaattgga aaataaccat
      721 tttttctctc ttttatacac acattcaaaa gaaagaaaaa aaatataccc cagcctcgac
      781 tagtgaattc gcggccatca caagtttgta caaaaaagca ggctgctagc gctaccggtc
      841 gccaccatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc
      901 ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc
      961 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc
     1021 ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg
     1081 aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg
     1141 gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg
     1201 caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc
     1261 cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag
     1321 gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac
     1381 gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac
     1441 aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag
     1501 tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta caagtccgga
     1561 gagggcagag gaagtcttct aacatgcggt gacgtggagg agaatcccgg ccctggggat
     1621 ccaccggtcg ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
     1681 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
     1741 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
     1801 gtgccctggc ccaccctcgt gaccaccctg tcctggggcg tgcagtgctt cgcccgctac
     1861 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
     1921 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
     1981 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     2041 aacatcctgg ggcacaagct ggagtacaac tactttagcg acaacgtcta tatcaccgcc
     2101 gacaagcaga agaacggcat caaggccaac ttcaagatcc gccacaacat cgaggacggc
     2161 ggcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     2221 ctgcccgaca accactacct gagcacccag tccaagctga gcaaagaccc caacgagaag
     2281 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
     2341 gagctgtaca agtaaagcgg ccgcacccag ctttcttgta caaagtggtg tagttaattc
     2401 atgatggccg ctgcaggtcg acctcgaggg ggggcccggt acccaattcg ccctatagtg
     2461 agtcgtatta cgcgcggatc cagctttgga cttcttcgcc agaggtttgg tcaagtctcc
     2521 aatcaaggtt gtcggcttgt ctaccttgcc agaaatttac gaaaagatgg aaaagggtca
     2581 aatcgttggt agatacgttg ttgacacttc taaataagcg aatttcttat gatttatgat
     2641 ttttattatt aaataagtta taaaaaaaat aagtgtatac aaattttaaa gtgactctta
     2701 ggttttaaaa cgaaaattct tattcttgag taactctttc ctgtaggtca ggttgctttc
     2761 tcaggtatag catgaggtcg ctcttattga ccacacctct accggcatgc caattcactg
     2821 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     2881 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     2941 tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt tctccttacg
     3001 catctgtgcg gtatttcaca ccgcataatc ggatcgtact tgttacccat cattgaattt
     3061 tgaacatccg aacctgggag ttttccctga aacagatagt atatttgaac ctgtataata
     3121 atatatagtc tagcgcttta cggaagacaa tgtatgtatt tcggttcctg gagaaactat
     3181 tgcatctatt gcataggtaa tcttgcacgt cgcatccccg gttcattttc tgcgtttcca
     3241 tcttgcactt caatagcata tctttgttaa cgaagcatct gtgcttcatt ttgtagaaca
     3301 aaaatgcaac gcgagagcgc taatttttca aacaaagaat ctgagctgca tttttacaga
     3361 acagaaatgc aacgcgaaag cgctatttta ccaacgaaga atctgtgctt catttttgta
     3421 aaacaaaaat gcaacgcgag agcgctaatt tttcaaacaa agaatctgag ctgcattttt
     3481 acagaacaga aatgcaacgc gagagcgcta ttttaccaac aaagaatcta tacttctttt
     3541 ttgttctaca aaaatgcatc ccgagagcgc tatttttcta acaaagcatc ttagattact
     3601 ttttttctcc tttgtgcgct ctataatgca gtctcttgat aactttttgc actgtaggtc
     3661 cgttaaggtt agaagaaggc tactttggtg tctattttct cttccataaa aaaagcctga
     3721 ctccacttcc cgcgtttact gattactagc gaagctgcgg gtgcattttt tcaagataaa
     3781 ggcatccccg attatattct ataccgatgt ggattgcgca tactttgtga acagaaagtg
     3841 atagcgttga tgattcttca ttggtcagaa aattatgaac ggtttcttct attttgtctc
     3901 tatatactac gtataggaaa tgtttacatt ttcgtattgt tttcgattca ctctatgaat
     3961 agttcttact acaatttttt tgtctaaaga gtaatactag agataaacat aaaaaatgta
     4021 gaggtcgagt ttagatgcaa gttcaaggag cgaaaggtgg atgggtaggt tatataggga
     4081 tatagcacag agatatatag caaagagata cttttgagca atgtttgtgg aagcggtatt
     4141 cgcaatattt tagtagctcg ttacagtccg gtgcgttttt ggttttttga aagtgcgtct
     4201 tcagagcgct tttggttttc aaaagcgctc tgaagttcct atactttcta gctagagaat
     4261 aggaacttcg gaataggaac ttcaaagcgt ttccgaaaac gagcgcttcc gaaaatgcaa
     4321 cgcgagctgc gcacatacag ctcactgttc acgtcgcacc tatatctgcg tgttgcctgt
     4381 atatatatat acatgagaag aacggcatag tgcgtgttta tgcttaaatg cgtacttata
     4441 tgcgtctatt tatgtaggat gaaaggtagt ctagtacctc ctgtgatatt atcccattcc
     4501 atgcggggta tcgtatgctt ccttcagcac taccctttag ctgttctata tgctgccact
     4561 cctcaattgg attagtctca tccttcaatg ctatcatttc ctttgatatt ggatcgatcc
     4621 gatgataagc tgtcaaacat gagaattggg taataactga tataattaaa ttgaagctct
     4681 aatttgtgag tttagtatac atgcatttac ttataataca gttttttagt tttgctggcc
     4741 gcatcttctc aaatatgctt cccagcctgc ttttctgtaa cgttcaccct ctaccttagc
     4801 atcccttccc tttgcaaata gtcctcttcc aacaataata atgtcagatc ctgtagagac
     4861 cacatcatcc acggttctat actgttgacc caatgcgtct cccttgtcat ctaaacccac
     4921 accgggtgtc ataatcaacc aatcgtaacc ttcatctctt ccacccatgt ctctttgagc
     4981 aataaagccg ataacaaaat ctttgtcgct cttcgcaatg tcaacagtac ccttagtata
     5041 ttctccagta gatagggagc ccttgcatga caattctgct aacatcaaaa ggcctctagg
     5101 ttcctttgtt acttcttctg ccgcctgctt caaaccgcta acaatacctg ggcccaccac
     5161 accgtgtgca ttcgtaatgt ctgcccattc tgctattctg tatacacccg cagagtactg
     5221 caatttgact gtattaccaa tgtcagcaaa ttttctgtct tcgaagagta aaaaattgta
     5281 cttggcggat aatgccttta gcggcttaac tgtgccctcc atggaaaaat cagtcaagat
     5341 atccacatgt gtttttagta aacaaatttt gggacctaat gcttcaacta actccagtaa
     5401 ttccttggtg gtacgaacat ccaatgaagc acacaagttt gtttgctttt cgtgcatgat
     5461 attaaatagc ttggcagcaa caggactagg atgagtagca gcacgttcct tatatgtagc
     5521 tttcgacatg atttatcttc gtttcctgca tgtttttgtt ctgtgcagtt gggttaagaa
     5581 tactgggcaa tttcatgttt cttcaacact acatatgcgt atatatacca atctaagtct
     5641 gtgctccttc cttcgttctt ccttctgttc ggagattacc gaatcaaaaa aatttcaagg
     5701 aaaccgaaat caaaaaaaag aataaaaaaa aaatgatgaa ttgaaaagct aattcttgaa
     5761 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     5821 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     5881 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     5941 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     6001 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     6061 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     6121 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     6181 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     6241 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     6301 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     6361 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     6421 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     6481 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     6541 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     6601 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     6661 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     6721 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     6781 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     6841 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     6901 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     6961 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     7021 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     7081 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     7141 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     7201 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     7261 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     7321 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     7381 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     7441 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     7501 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     7561 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     7621 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     7681 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     7741 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     7801 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     7861 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     7921 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     7981 accatgatta cgccaagctt accgcatcag gaaattgtaa gcgttaatat tttgttaaaa
     8041 ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa
     8101 atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac
     8161 aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag
     8221 ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt
     8281 aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg
     8341 gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca
     8401 agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag
     8461 ggcgcgtcca ttcgc
//
