LOCUS       sequence-236372-        8457 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Produces equimolar expression of mKo2 and mTq2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8457)
  AUTHORS   Botman D, de Groot DH, Schmidt P, Goedhart J, Teusink B
  TITLE     In vivo characterisation of fluorescent proteins in budding yeast.
  JOURNAL   Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.1038/s41598-019-38913-z.
  PUBMED    30783202
REFERENCE   2  (bases 1 to 8457)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2019-02-19"; volume: "9(1):2234. doi"; pages: " 
            10.1038/s41598-019-38913-z"
COMMENT     Sequence Label: pDRF1-GW mKo2-T2A-mTurquoise2
FEATURES             Location/Qualifiers
     source          1..8457
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    800..824
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      841..850
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             868..1515
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="SVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRVTM
                     AEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSASV
                     SAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMYLK
                     LEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
     CDS             1543..1596
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      1612..1621
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1618..1620,1621..1623,1624..2337)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1663..1684)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1924..1943)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2271..2292
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(2347..2371)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(2433..2452)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2434..2452)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      2600..2787
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2655..2679)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(2799..2816)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2799..2815)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2808..2830)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3251..4597
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(4708..5511)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(5512..5727)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(5752..5770)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5838..5942
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5943..6011,6012..6803)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6161..6180)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6974..7562
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7463..7482
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7716..7733
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7850..7871
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7886..7891,7892..7909,7910..7916)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7924..7940
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7929..7951
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7948..7964
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7948..7964
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(7994..8449)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8131..8152)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8343..8362
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 caagcttcct gaaacggaga aacataaaca ggcattgctg ggatcaccca tacatcactc
       61 tgttttgcct gaccttttcc ggtaatttga aaacaaaccc ggtctcgaag cggagatccg
      121 gcgataatta ccgcagaaat aaacccatac acgagacgta gaaccagccg cacatggccg
      181 gagaaactcc tgcgagaatt tcgtaaactc gcgcgcattg catctgtatt tcctaatgcg
      241 gcacttccag gcctcgatcg agaccgttta tccattgctt ttttgttgtc tttttccctc
      301 gttcacagaa agtctgaaga agctatagta gaactatgag ctttttttgt ttctgttttc
      361 cttttttttt tttttacctc tgtggaaatt gttactctca cactctttag ttcgtttgtt
      421 tgttttgttt attccaatta tgaccggtga cgaaacgtgg tcgatggtgg gtaccgctta
      481 tgctcccctc cattagtttc gattatataa aaaggccaaa tattgtatta ttttcaaatg
      541 tcctatcatt atcgtctaac atctaatttc tcttaaattt tttctctttc tttcctataa
      601 caccaatagt gaaaatcttt ttttcttcta tatctacaaa aacttttttt ttctatcaac
      661 ctcgttgata aattttttct ttaacaatcg ttaataatta attaattgga aaataaccat
      721 tttttctctc ttttatacac acattcaaaa gaaagaaaaa aaatataccc cagcctcgac
      781 tagtgaattc gcggccatca caagtttgta caaaaaagca ggctgctagc gctaccggtc
      841 gccaccatgg tgagcaaggg cgaggagagt gtgattaaac cagagatgaa gatgaggtac
      901 tacatggacg gctccgtcaa tgggcatgag ttcacaattg aaggtgaagg cacaggcaga
      961 ccttacgagg gacatcaaga gatgacacta cgcgtcacaa tggccgaggg cgggccaatg
     1021 cctttcgcgt ttgacttagt gtcacacgtg ttctgttacg gccacagagt atttactaaa
     1081 tatccagaag agataccaga ctatttcaaa caagcatttc ctgaaggcct gtcatgggaa
     1141 aggtcgttgg agttcgaaga tggtgggtcc gcttcagtca gtgcgcatat aagccttaga
     1201 ggaaacacct tctaccacaa atccaaattt actggggtta actttcctgc cgatggtcct
     1261 atcatgcaaa accaaagtgt tgattgggag ccatcaaccg agaaaattac tgccagcgac
     1321 ggagttctga agggtgatgt tacgatgtac ctaaaacttg aaggaggcgg caatcacaaa
     1381 tgccaaatga agactactta caaggcggca aaagagattc ttgaaatgcc aggagaccat
     1441 tacatcggcc atcgcctcgt caggaaaacc gaaggcaaca ttactgagca ggtagaagat
     1501 gcagtagctc attccactgg cgacgagctg tacaagtccg gagagggcag aggaagtctt
     1561 ctaacatgcg gtgacgtgga ggagaatccc ggccctgggg atccaccggt cgccaccatg
     1621 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
     1681 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
     1741 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     1801 gtgaccaccc tgtcctgggg cgtgcagtgc ttcgcccgct accccgacca catgaagcag
     1861 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
     1921 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     1981 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     2041 ctggagtaca actactttag cgacaacgtc tatatcaccg ccgacaagca gaagaacggc
     2101 atcaaggcca acttcaagat ccgccacaac atcgaggacg gcggcgtgca gctcgccgac
     2161 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     2221 ctgagcaccc agtccaagct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     2281 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaaagc
     2341 ggccgcaccc agctttcttg tacaaagtgg tgtagttaat tcatgatggc cgctgcaggt
     2401 cgacctcgag ggggggcccg gtacccaatt cgccctatag tgagtcgtat tacgcgcgga
     2461 tccagctttg gacttcttcg ccagaggttt ggtcaagtct ccaatcaagg ttgtcggctt
     2521 gtctaccttg ccagaaattt acgaaaagat ggaaaagggt caaatcgttg gtagatacgt
     2581 tgttgacact tctaaataag cgaatttctt atgatttatg atttttatta ttaaataagt
     2641 tataaaaaaa ataagtgtat acaaatttta aagtgactct taggttttaa aacgaaaatt
     2701 cttattcttg agtaactctt tcctgtaggt caggttgctt tctcaggtat agcatgaggt
     2761 cgctcttatt gaccacacct ctaccggcat gccaattcac tggccgtcgt tttacaacgt
     2821 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     2881 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     2941 ctgaatggcg aatggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     3001 caccgcataa tcggatcgta cttgttaccc atcattgaat tttgaacatc cgaacctggg
     3061 agttttccct gaaacagata gtatatttga acctgtataa taatatatag tctagcgctt
     3121 tacggaagac aatgtatgta tttcggttcc tggagaaact attgcatcta ttgcataggt
     3181 aatcttgcac gtcgcatccc cggttcattt tctgcgtttc catcttgcac ttcaatagca
     3241 tatctttgtt aacgaagcat ctgtgcttca ttttgtagaa caaaaatgca acgcgagagc
     3301 gctaattttt caaacaaaga atctgagctg catttttaca gaacagaaat gcaacgcgaa
     3361 agcgctattt taccaacgaa gaatctgtgc ttcatttttg taaaacaaaa atgcaacgcg
     3421 agagcgctaa tttttcaaac aaagaatctg agctgcattt ttacagaaca gaaatgcaac
     3481 gcgagagcgc tattttacca acaaagaatc tatacttctt ttttgttcta caaaaatgca
     3541 tcccgagagc gctatttttc taacaaagca tcttagatta ctttttttct cctttgtgcg
     3601 ctctataatg cagtctcttg ataacttttt gcactgtagg tccgttaagg ttagaagaag
     3661 gctactttgg tgtctatttt ctcttccata aaaaaagcct gactccactt cccgcgttta
     3721 ctgattacta gcgaagctgc gggtgcattt tttcaagata aaggcatccc cgattatatt
     3781 ctataccgat gtggattgcg catactttgt gaacagaaag tgatagcgtt gatgattctt
     3841 cattggtcag aaaattatga acggtttctt ctattttgtc tctatatact acgtatagga
     3901 aatgtttaca ttttcgtatt gttttcgatt cactctatga atagttctta ctacaatttt
     3961 tttgtctaaa gagtaatact agagataaac ataaaaaatg tagaggtcga gtttagatgc
     4021 aagttcaagg agcgaaaggt ggatgggtag gttatatagg gatatagcac agagatatat
     4081 agcaaagaga tacttttgag caatgtttgt ggaagcggta ttcgcaatat tttagtagct
     4141 cgttacagtc cggtgcgttt ttggtttttt gaaagtgcgt cttcagagcg cttttggttt
     4201 tcaaaagcgc tctgaagttc ctatactttc tagctagaga ataggaactt cggaatagga
     4261 acttcaaagc gtttccgaaa acgagcgctt ccgaaaatgc aacgcgagct gcgcacatac
     4321 agctcactgt tcacgtcgca cctatatctg cgtgttgcct gtatatatat atacatgaga
     4381 agaacggcat agtgcgtgtt tatgcttaaa tgcgtactta tatgcgtcta tttatgtagg
     4441 atgaaaggta gtctagtacc tcctgtgata ttatcccatt ccatgcgggg tatcgtatgc
     4501 ttccttcagc actacccttt agctgttcta tatgctgcca ctcctcaatt ggattagtct
     4561 catccttcaa tgctatcatt tcctttgata ttggatcgat ccgatgataa gctgtcaaac
     4621 atgagaattg ggtaataact gatataatta aattgaagct ctaatttgtg agtttagtat
     4681 acatgcattt acttataata cagtttttta gttttgctgg ccgcatcttc tcaaatatgc
     4741 ttcccagcct gcttttctgt aacgttcacc ctctacctta gcatcccttc cctttgcaaa
     4801 tagtcctctt ccaacaataa taatgtcaga tcctgtagag accacatcat ccacggttct
     4861 atactgttga cccaatgcgt ctcccttgtc atctaaaccc acaccgggtg tcataatcaa
     4921 ccaatcgtaa ccttcatctc ttccacccat gtctctttga gcaataaagc cgataacaaa
     4981 atctttgtcg ctcttcgcaa tgtcaacagt acccttagta tattctccag tagataggga
     5041 gcccttgcat gacaattctg ctaacatcaa aaggcctcta ggttcctttg ttacttcttc
     5101 tgccgcctgc ttcaaaccgc taacaatacc tgggcccacc acaccgtgtg cattcgtaat
     5161 gtctgcccat tctgctattc tgtatacacc cgcagagtac tgcaatttga ctgtattacc
     5221 aatgtcagca aattttctgt cttcgaagag taaaaaattg tacttggcgg ataatgcctt
     5281 tagcggctta actgtgccct ccatggaaaa atcagtcaag atatccacat gtgtttttag
     5341 taaacaaatt ttgggaccta atgcttcaac taactccagt aattccttgg tggtacgaac
     5401 atccaatgaa gcacacaagt ttgtttgctt ttcgtgcatg atattaaata gcttggcagc
     5461 aacaggacta ggatgagtag cagcacgttc cttatatgta gctttcgaca tgatttatct
     5521 tcgtttcctg catgtttttg ttctgtgcag ttgggttaag aatactgggc aatttcatgt
     5581 ttcttcaaca ctacatatgc gtatatatac caatctaagt ctgtgctcct tccttcgttc
     5641 ttccttctgt tcggagatta ccgaatcaaa aaaatttcaa ggaaaccgaa atcaaaaaaa
     5701 agaataaaaa aaaaatgatg aattgaaaag ctaattcttg aagacgaaag ggcctcgtga
     5761 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     5821 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     5881 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     5941 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     6001 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     6061 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     6121 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     6181 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     6241 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     6301 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     6361 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     6421 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     6481 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     6541 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     6601 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     6661 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     6721 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     6781 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     6841 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     6901 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     6961 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     7021 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     7081 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     7141 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     7201 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     7261 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     7321 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     7381 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     7441 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     7501 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     7561 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     7621 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     7681 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     7741 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     7801 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     7861 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     7921 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
     7981 ttaccgcatc aggaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt
     8041 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa
     8101 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag
     8161 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt
     8221 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac
     8281 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
     8341 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg
     8401 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc cattcgc
//
