LOCUS       sequence-236376-        2162 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly AB part for pTra* (improved pTra from 
            DOI: 10.1021/acssynbio.5b00286). Please see Supplemental Documents 
            for annotated Genbank file..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2162)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: P38m
FEATURES             Location/Qualifiers
     source          1..2162
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(59..850,851..919))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     682..701
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(920..1024)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1092..1110
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1114..1157)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    1160..1181
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    1265..1285
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(1291..1308)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1462..2050)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1542..1561)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
       61 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
      121 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
      181 gtgctgcaat gataccgcgc gacccacgct caccggctcc agatttatca gcaataaacc
      241 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
      301 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
      361 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
      421 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
      481 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
      541 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
      601 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
      661 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
      721 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
      781 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
      841 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
      901 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
      961 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     1021 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
     1081 taacctataa aaataggcgt atcacgaggc agaatttcag ataaaaaaaa tccttagctt
     1141 tcgctaagga tgatttctgg aattcgcggc cgcttctaga gactagtggg tctcaggagg
     1201 cacgtgcaga tctgcacatt tacgcaagaa aatggtttgt tatagtcgaa tattactaga
     1261 gacctactag tagcggccgc tgcaggcttc ctcgctcact gactcgctgc gctcggtcgt
     1321 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     1381 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     1441 aaaggccgcg ttgctggcgt ttttccacag gctccgcccc cctgacgagc atcacaaaaa
     1501 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     1561 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     1621 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     1681 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     1741 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     1801 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     1861 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     1921 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     1981 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     2041 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     2101 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     2161 aa
//
