LOCUS       sequence-236378-        2164 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly AB part for pRpa* (improved pRpa from 
            DOI: 10.1021/acssynbio.5b00286). Please see Supplemental Documents 
            for annotated Genbank file..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2164)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: P37m
FEATURES             Location/Qualifiers
     source          1..2164
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     16..34
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(38..81)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    84..105
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    191..211
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(217..234)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(388..976)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(468..487)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1147..1938,1939..2007))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1770..1789
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2008..2112)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 acattaacct ataaaaatag gcgtatcacg aggcagaatt tcagataaaa aaaatcctta
       61 gctttcgcta aggatgattt ctggaattcg cggccgcttc tagagactag tgggtctcag
      121 gaggcacctg tccgatcgga cagtattacg caagaaaatg gtttgttata gtcgaatatt
      181 actagagacc tactagtagc ggccgctgca ggcttcctcg ctcactgact cgctgcgctc
      241 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
      301 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
      361 ccgtaaaaag gccgcgttgc tggcgttttt ccacaggctc cgcccccctg acgagcatca
      421 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
      481 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
      541 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
      601 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
      661 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
      721 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
      781 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
      841 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
      901 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
      961 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     1021 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     1081 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     1141 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     1201 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     1261 tggccccagt gctgcaatga taccgcgcga cccacgctca ccggctccag atttatcagc
     1321 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     1381 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     1441 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     1501 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     1561 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     1621 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     1681 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     1741 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     1801 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     1861 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     1921 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     1981 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     2041 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     2101 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
     2161 catg
//
