LOCUS       sequence-236383-        2210 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly DE part for gQi gRNA (guide RNA for S. 
            pyogenes Cas9; sequence from DOI: 10.1016/j.cell.2013.02.022). 
            Please see Supplemental Documents for annotated Genbank file..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2210)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: UC16m
FEATURES             Location/Qualifiers
     source          1..2210
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(90..109)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(769..1560,1561..1629))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1392..1411
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1630..1734)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1802..1820
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1824..1867)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    1870..1891
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_RNA        1930..2005
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    2023..2043
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(2049..2066)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gctggcgttt ttccacaggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
       61 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
      121 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
      181 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
      241 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
      301 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
      361 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
      421 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
      481 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
      541 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
      601 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
      661 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
      721 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
      781 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
      841 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
      901 gataccgcgc gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
      961 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     1021 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     1081 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     1141 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     1201 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     1261 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     1321 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     1381 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     1441 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     1501 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     1561 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     1621 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     1681 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     1741 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
     1801 aaataggcgt atcacgaggc agaatttcag ataaaaaaaa tccttagctt tcgctaagga
     1861 tgatttctgg aattcgcggc cgcttctaga gactagtggg tctcatacta actttcagtt
     1921 tagcggtctg ttttagagct agaaatagca agttaaaata aggctagtcc gttatcaact
     1981 tgaaaaagtg gcaccgagtc ggtgcttttt taggtagaga cctactagta gcggccgctg
     2041 caggcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     2101 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     2161 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
//
