LOCUS       sequence-236387-        2729 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly CD part for TetR (tet repressor; 
            identical to C2d but without ssrA degradation tag). Please see 
            Supplemental Documents for annotated Genbank file..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2729)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: C27m
FEATURES             Location/Qualifiers
     source          1..2729
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     35..53
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(57..100)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    103..124
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     CDS             140..763
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(213..232)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     misc_feature    775..795
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(801..818)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(972..1560)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1052..1071)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1731..2522,2523..2591))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2354..2373
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2592..2696)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggcagaattt
       61 cagataaaaa aaatccttag ctttcgctaa ggatgatttc tggaattcgc ggccgcttct
      121 agagactagt gggtctcaaa tgtccagatt agataaaagt aaagtgatta acagcgcatt
      181 agagctgctt aatgaggtcg gaatcgaagg tttaacaacc cgtaaactcg cccagaagct
      241 aggtgtagag cagcctacat tgtattggca tgtaaaaaat aagcgggctt tgctcgacgc
      301 cttagccatt gagatgttag ataggcacca tactcacttt tgccctttag aaggggaaag
      361 ctggcaagat tttttacgta ataacgctaa aagttttaga tgtgctttac taagtcatcg
      421 cgatggagca aaagtacatt taggtacacg gcctacagaa aaacagtatg aaactctcga
      481 aaatcaatta gcctttttat gccaacaagg tttttcacta gagaatgcat tatatgcact
      541 cagcgctgtg gggcatttta ctttaggttg cgtattggaa gatcaagagc atcaagtcgc
      601 taaagaagaa agggaaacac ctactactga tagtatgccg ccattattac gacaagctat
      661 cgaattattt gatcaccaag gtgcagagcc agccttctta ttcggccttg aattgatcat
      721 atgcggatta gaaaaacaac ttaaatgtga aagtgggtcc taaaggtaga gacctactag
      781 tagcggccgc tgcaggcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
      841 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
      901 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
      961 ttgctggcgt ttttccacag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     1021 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     1081 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     1141 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     1201 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     1261 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     1321 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     1381 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     1441 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     1501 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     1561 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     1621 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     1681 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     1741 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     1801 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     1861 atgataccgc gcgacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     1921 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     1981 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     2041 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     2101 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     2161 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     2221 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     2281 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     2341 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     2401 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     2461 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     2521 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     2581 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     2641 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     2701 tttccccgaa aagtgccacc tgacgtcta
//
