LOCUS       sequence-236389-        8461 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Produces equimolar expression of mTFP and mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8461)
  AUTHORS   Botman D, de Groot DH, Schmidt P, Goedhart J, Teusink B
  TITLE     In vivo characterisation of fluorescent proteins in budding yeast.
  JOURNAL   Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.1038/s41598-019-38913-z.
  PUBMED    30783202
REFERENCE   2  (bases 1 to 8461)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2019-02-19"; volume: "9(1):2234. doi"; pages: " 
            10.1038/s41598-019-38913-z"
COMMENT     Sequence Label: pDRF1-GW mTFP-T2A-mCherry
FEATURES             Location/Qualifiers
     source          1..8461
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    800..824
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             842..1549
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
     CDS             1556..1609
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      1625..1634
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1631..2341
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1776..1794)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     protein_bind    complement(2351..2375)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(2437..2456)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2438..2456)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      2604..2791
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2659..2683)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(2803..2820)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2803..2819)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2812..2834)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3255..4601
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(4712..5515)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(5516..5731)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(5756..5774)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5842..5946
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5947..6015,6016..6807)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6165..6184)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6978..7566
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7467..7486
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7720..7737
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7854..7875
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7890..7895,7896..7913,7914..7920)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7928..7944
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7933..7955
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7952..7968
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7952..7968
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(7998..8453)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8135..8156)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8347..8366
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 caagcttcct gaaacggaga aacataaaca ggcattgctg ggatcaccca tacatcactc
       61 tgttttgcct gaccttttcc ggtaatttga aaacaaaccc ggtctcgaag cggagatccg
      121 gcgataatta ccgcagaaat aaacccatac acgagacgta gaaccagccg cacatggccg
      181 gagaaactcc tgcgagaatt tcgtaaactc gcgcgcattg catctgtatt tcctaatgcg
      241 gcacttccag gcctcgatcg agaccgttta tccattgctt ttttgttgtc tttttccctc
      301 gttcacagaa agtctgaaga agctatagta gaactatgag ctttttttgt ttctgttttc
      361 cttttttttt tttttacctc tgtggaaatt gttactctca cactctttag ttcgtttgtt
      421 tgttttgttt attccaatta tgaccggtga cgaaacgtgg tcgatggtgg gtaccgctta
      481 tgctcccctc cattagtttc gattatataa aaaggccaaa tattgtatta ttttcaaatg
      541 tcctatcatt atcgtctaac atctaatttc tcttaaattt tttctctttc tttcctataa
      601 caccaatagt gaaaatcttt ttttcttcta tatctacaaa aacttttttt ttctatcaac
      661 ctcgttgata aattttttct ttaacaatcg ttaataatta attaattgga aaataaccat
      721 tttttctctc ttttatacac acattcaaaa gaaagaaaaa aaatataccc cagcctcgac
      781 tagtgaattc gcggccatca caagtttgta caaaaaagca ggctgctagc gctaccggtc
      841 gatggtgagc aagggcgagg agaccacaat gggcgtaatc aagcccgaca tgaagatcaa
      901 gctgaagatg gagggcaacg tgaatggcca cgccttcgtg atcgagggcg agggcgaggg
      961 caagccctac gacggcacca acaccatcaa cctggaggtg aaggagggag cccccctgcc
     1021 cttctcctac gacattctga ccaccgcgtt cgcctacggc aacagggcct tcaccaagta
     1081 ccccgacgac atccccaact acttcaagca gtccttcccc gagggctact cttgggagcg
     1141 caccatgacc ttcgaggaca agggcatcgt gaaggtgaag tccgacatct ccatggagga
     1201 ggactccttc atctacgaga tacacctcaa gggcgagaac ttccccccca acggccccgt
     1261 gatgcagaag aagaccaccg gctgggacgc ctccaccgag aggatgtacg tgcgcgacgg
     1321 cgtgctgaag ggcgacgtca agcacaagct gctgctggag ggcggcggcc accaccgcgt
     1381 tgacttcaag accatctaca gggccaagaa ggcggtgaag ctgcccgact atcactttgt
     1441 ggaccaccgc atcgagatcc tgaaccacga caaggactac aacaaggtga ccgtttacga
     1501 gagcgccgtg gcccgcaact ccaccgacgg catggacgag ctgtacaagt ccggagaggg
     1561 cagaggaagt cttctaacat gcggtgacgt ggaggagaat cccggccctg gggatccacc
     1621 ggtcgccacc atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat
     1681 gcgcttcaag gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga
     1741 gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg
     1801 ccccctgccc ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta
     1861 cgtgaagcac cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa
     1921 gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc
     1981 cctgcaggac ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga
     2041 cggccccgta atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc
     2101 cgaggacggc gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca
     2161 ctacgacgct gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc
     2221 ctacaacgtc aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga
     2281 acagtacgaa cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaagta
     2341 aagcggccgc acccagcttt cttgtacaaa gtggtgtagt taattcatga tggccgctgc
     2401 aggtcgacct cgaggggggg cccggtaccc aattcgccct atagtgagtc gtattacgcg
     2461 cggatccagc tttggacttc ttcgccagag gtttggtcaa gtctccaatc aaggttgtcg
     2521 gcttgtctac cttgccaaaa atttacgaaa agatggaaaa gggtcaaatc gttggtagat
     2581 acgttgttga cacttctaaa taagcgaatt tcttatgatt tatgattttt attattaaat
     2641 aagttataaa aaaaataagt gtatacaaat tttaaagtga ctcttaggtt ttaaaacgaa
     2701 aattcttatt cttgagtaac tctttcctgt aggtcaggtt gctttctcag gtatagcatg
     2761 aggtcgctct tattgaccac acctctaccg gcatgccaat tcactggccg tcgttttaca
     2821 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     2881 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     2941 cagcctgaat ggcgaatggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     3001 ttcacaccgc ataatcggat cgtacttgtt acccatcatt gaattttgaa catccgaacc
     3061 tgggagtttt ccctgaaaca gatagtatat ttgaacctgt ataataatat atagtctagc
     3121 gctttacgga agacaatgta tgtatttcgg ttcctggaga aactattgca tctattgcat
     3181 aggtaatctt gcacgtcgca tccccggttc attttctgcg tttccatctt gcacttcaat
     3241 agcatatctt tgttaacgaa gcatctgtgc ttcattttgt agaacaaaaa tgcaacgcga
     3301 gagcgctaat ttttcaaaca aagaatctga gctgcatttt tacagaacag aaatgcaacg
     3361 cgaaagcgct attttaccaa cgaagaatct gtgcttcatt tttgtaaaac aaaaatgcaa
     3421 cgcgagagcg ctaatttttc aaacaaagaa tctgagctgc atttttacag aacagaaatg
     3481 caacgcgaga gcgctatttt accaacaaag aatctatact tcttttttgt tctacaaaaa
     3541 tgcatcccga gagcgctatt tttctaacaa agcatcttag attacttttt ttctcctttg
     3601 tgcgctctat aatgcagtct cttgataact ttttgcactg taggtccgtt aaggttagaa
     3661 gaaggctact ttggtgtcta ttttctcttc cataaaaaaa gcctgactcc acttcccgcg
     3721 tttactgatt actagcgaag ctgcgggtgc attttttcaa gataaaggca tccccgatta
     3781 tattctatac cgatgtggat tgcgcatact ttgtgaacag aaagtgatag cgttgatgat
     3841 tcttcattgg tcagaaaatt atgaacggtt tcttctattt tgtctctata tactacgtat
     3901 aggaaatgtt tacattttcg tattgttttc gattcactct atgaatagtt cttactacaa
     3961 tttttttgtc taaagagtaa tactagagat aaacataaaa aatgtagagg tcgagtttag
     4021 atgcaagttc aaggagcgaa aggtggatgg gtaggttata tagggatata gcacagagat
     4081 atatagcaaa gagatacttt tgagcaatgt ttgtggaagc ggtattcgca atattttagt
     4141 agctcgttac agtccggtgc gtttttggtt ttttgaaagt gcgtcttcag agcgcttttg
     4201 gttttcaaaa gcgctctgaa gttcctatac tttctagcta gagaatagga acttcggaat
     4261 aggaacttca aagcgtttcc gaaaacgagc gcttccgaaa atgcaacgcg agctgcgcac
     4321 atacagctca ctgttcacgt cgcacctata tctgcgtgtt gcctgtatat atatatacat
     4381 gagaagaacg gcatagtgcg tgtttatgct taaatgcgta cttatatgcg tctatttatg
     4441 taggatgaaa ggtagtctag tacctcctgt gatattatcc cattccatgc ggggtatcgt
     4501 atgcttcctt cagcactacc ctttagctgt tctatatgct gccactcctc aattggatta
     4561 gtctcatcct tcaatgctat catttccttt gatattggat cgatccgatg ataagctgtc
     4621 aaacatgaga attgggtaat aactgatata attaaattga agctctaatt tgtgagttta
     4681 gtatacatgc atttacttat aatacagttt tttagttttg ctggccgcat cttctcaaat
     4741 atgcttccca gcctgctttt ctgtaacgtt caccctctac cttagcatcc cttccctttg
     4801 caaatagtcc tcttccaaca ataataatgt cagatcctgt agagaccaca tcatccacgg
     4861 ttctatactg ttgacccaat gcgtctccct tgtcatctaa acccacaccg ggtgtcataa
     4921 tcaaccaatc gtaaccttca tctcttccac ccatgtctct ttgagcaata aagccgataa
     4981 caaaatcttt gtcgctcttc gcaatgtcaa cagtaccctt agtatattct ccagtagata
     5041 gggagccctt gcatgacaat tctgctaaca tcaaaaggcc tctaggttcc tttgttactt
     5101 cttctgccgc ctgcttcaaa ccgctaacaa tacctgggcc caccacaccg tgtgcattcg
     5161 taatgtctgc ccattctgct attctgtata cacccgcaga gtactgcaat ttgactgtat
     5221 taccaatgtc agcaaatttt ctgtcttcga agagtaaaaa attgtacttg gcggataatg
     5281 cctttagcgg cttaactgtg ccctccatgg aaaaatcagt caagatatcc acatgtgttt
     5341 ttagtaaaca aattttggga cctaatgctt caactaactc cagtaattcc ttggtggtac
     5401 gaacatccaa tgaagcacac aagtttgttt gcttttcgtg catgatatta aatagcttgg
     5461 cagcaacagg actaggatga gtagcagcac gttccttata tgtagctttc gacatgattt
     5521 atcttcgttt cctgcatgtt tttgttctgt gcagttgggt taagaatact gggcaatttc
     5581 atgtttcttc aacactacat atgcgtatat ataccaatct aagtctgtgc tccttccttc
     5641 gttcttcctt ctgttcggag attaccgaat caaaaaaatt tcaaggaaac cgaaatcaaa
     5701 aaaaagaata aaaaaaaaat gatgaattga aaagctaatt cttgaagacg aaagggcctc
     5761 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     5821 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     5881 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     5941 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     6001 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     6061 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     6121 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     6181 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     6241 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     6301 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     6361 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     6421 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     6481 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     6541 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     6601 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     6661 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     6721 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     6781 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     6841 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     6901 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     6961 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     7021 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     7081 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     7141 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     7201 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     7261 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     7321 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     7381 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     7441 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     7501 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     7561 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     7621 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     7681 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     7741 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     7801 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
     7861 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
     7921 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc
     7981 aagcttaccg catcaggaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt
     8041 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
     8101 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
     8161 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
     8221 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
     8281 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
     8341 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
     8401 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtccattcg
     8461 c
//
