LOCUS       sequence-236397-        8484 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Produces equimolar expression of sYFP2 and mTq2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8484)
  AUTHORS   Botman D, de Groot DH, Schmidt P, Goedhart J, Teusink B
  TITLE     In vivo characterisation of fluorescent proteins in budding yeast.
  JOURNAL   Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.1038/s41598-019-38913-z.
  PUBMED    30783202
REFERENCE   2  (bases 1 to 8484)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2019-02-19"; volume: "9(1):2234. doi"; pages: " 
            10.1038/s41598-019-38913-z"
COMMENT     Sequence Label: pDRF1-GW sYFP2-T2A-mTurquoise2
FEATURES             Location/Qualifiers
     source          1..8484
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    800..824
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      841..850
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(847..849,850..852,853..1563)
                     /codon_start=1
                     /product="monomeric optimized variant of EYFP, identical to
                     mVenus but with L68V (Kremers et al., 2006)"
                     /label=SYFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             1570..1623
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      1639..1648
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1645..1647,1648..1650,1651..2364)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     protein_bind    complement(2374..2398)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(2460..2479)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2461..2479)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      2627..2814
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2682..2706)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(2826..2843)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2826..2842)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2835..2857)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3278..4624
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(4735..5538)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(5539..5754)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(5779..5797)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5865..5969
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5970..6038,6039..6830)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6188..6207)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7001..7589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7490..7509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7743..7760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7877..7898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7913..7918,7919..7936,7937..7943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7951..7967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7956..7978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7975..7991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7975..7991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(8021..8476)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8158..8179)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8370..8389
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 caagcttcct gaaacggaga aacataaaca ggcattgctg ggatcaccca tacatcactc
       61 tgttttgcct gaccttttcc ggtaatttga aaacaaaccc ggtctcgaag cggagatccg
      121 gcgataatta ccgcagaaat aaacccatac acgagacgta gaaccagccg cacatggccg
      181 gagaaactcc tgcgagaatt tcgtaaactc gcgcgcattg catctgtatt tcctaatgcg
      241 gcacttccag gcctcgatcg agaccgttta tccattgctt ttttgttgtc tttttccctc
      301 gttcacagaa agtctgaaga agctatagta gaactatgag ctttttttgt ttctgttttc
      361 cttttttttt tttttacctc tgtggaaatt gttactctca cactctttag ttcgtttgtt
      421 tgttttgttt attccaatta tgaccggtga cgaaacgtgg tcgatggtgg gtaccgctta
      481 tgctcccctc cattagtttc gattatataa aaaggccaaa tattgtatta ttttcaaatg
      541 tcctatcatt atcgtctaac atctaatttc tcttaaattt tttctctttc tttcctataa
      601 caccaatagt gaaaatcttt ttttcttcta tatctacaaa aacttttttt ttctatcaac
      661 ctcgttgata aattttttct ttaacaatcg ttaataatta attaattgga aaataaccat
      721 tttttctctc ttttatacac acattcaaaa gaaagaaaaa aaatataccc cagcctcgac
      781 tagtgaattc gcggccatca caagtttgta caaaaaagca ggctgctagc gctaccggtc
      841 gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
      901 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
      961 acctacggca agctgaccct gaagctgatc tgcaccaccg gcaagctgcc cgtgccctgg
     1021 cccaccctcg tgaccaccct gggctacggc gtgcagtgct tcgcccgcta ccccgaccac
     1081 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     1141 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
     1201 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     1261 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcaccgc cgacaagcag
     1321 aagaacggca tcaaggccaa cttcaagatc cgccacaaca tcgaggacgg cggcgtgcag
     1381 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     1441 aaccactacc tgagctacca gtccaagctg agcaaagacc ccaacgagaa gcgcgatcac
     1501 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
     1561 aagtccggag agggcagagg aagtcttcta acatgcggtg acgtggagga gaatcccggc
     1621 cctggggatc caccggtcgc caccatggtg agcaagggcg aggagctgtt caccggggtg
     1681 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     1741 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     1801 aagctgcccg tgccctggcc caccctcgtg accaccctgt cctggggcgt gcagtgcttc
     1861 gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     1921 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     1981 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     2041 gaggacggca acatcctggg gcacaagctg gagtacaact actttagcga caacgtctat
     2101 atcaccgccg acaagcagaa gaacggcatc aaggccaact tcaagatccg ccacaacatc
     2161 gaggacggcg gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     2221 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccaagctgag caaagacccc
     2281 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     2341 ggcatggacg agctgtacaa gtaaagcggc cgcacccagc tttcttgtac aaagtggtgt
     2401 agttaattca tgatggccgc tgcaggtcga cctcgagggg gggcccggta cccaattcgc
     2461 cctatagtga gtcgtattac gcgcggatcc agctttggac ttcttcgcca gaggtttggt
     2521 caagtctcca atcaaggttg tcggcttgtc taccttgcca gaaatttacg aaaagatgga
     2581 aaagggtcaa atcgttggta gatacgttgt tgacacttct aaataagcga atttcttatg
     2641 atttatgatt tttattatta aataagttat aaaaaaaata agtgtataca aattttaaag
     2701 tgactcttag gttttaaaac gaaaattctt attcttgagt aactctttcc tgtaggtcag
     2761 gttgctttct caggtatagc atgaggtcgc tcttattgac cacacctcta ccggcatgcc
     2821 aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     2881 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     2941 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt
     3001 ctccttacgc atctgtgcgg tatttcacac cgcataatcg gatcgtactt gttacccatc
     3061 attgaatttt gaacatccga acctgggagt tttccctgaa acagatagta tatttgaacc
     3121 tgtataataa tatatagtct agcgctttac ggaagacaat gtatgtattt cggttcctgg
     3181 agaaactatt gcatctattg cataggtaat cttgcacgtc gcatccccgg ttcattttct
     3241 gcgtttccat cttgcacttc aatagcatat ctttgttaac gaagcatctg tgcttcattt
     3301 tgtagaacaa aaatgcaacg cgagagcgct aatttttcaa acaaagaatc tgagctgcat
     3361 ttttacagaa cagaaatgca acgcgaaagc gctattttac caacgaagaa tctgtgcttc
     3421 atttttgtaa aacaaaaatg caacgcgaga gcgctaattt ttcaaacaaa gaatctgagc
     3481 tgcattttta cagaacagaa atgcaacgcg agagcgctat tttaccaaca aagaatctat
     3541 acttcttttt tgttctacaa aaatgcatcc cgagagcgct atttttctaa caaagcatct
     3601 tagattactt tttttctcct ttgtgcgctc tataatgcag tctcttgata actttttgca
     3661 ctgtaggtcc gttaaggtta gaagaaggct actttggtgt ctattttctc ttccataaaa
     3721 aaagcctgac tccacttccc gcgtttactg attactagcg aagctgcggg tgcatttttt
     3781 caagataaag gcatccccga ttatattcta taccgatgtg gattgcgcat actttgtgaa
     3841 cagaaagtga tagcgttgat gattcttcat tggtcagaaa attatgaacg gtttcttcta
     3901 ttttgtctct atatactacg tataggaaat gtttacattt tcgtattgtt ttcgattcac
     3961 tctatgaata gttcttacta caattttttt gtctaaagag taatactaga gataaacata
     4021 aaaaatgtag aggtcgagtt tagatgcaag ttcaaggagc gaaaggtgga tgggtaggtt
     4081 atatagggat atagcacaga gatatatagc aaagagatac ttttgagcaa tgtttgtgga
     4141 agcggtattc gcaatatttt agtagctcgt tacagtccgg tgcgtttttg gttttttgaa
     4201 agtgcgtctt cagagcgctt ttggttttca aaagcgctct gaagttccta tactttctag
     4261 ctagagaata ggaacttcgg aataggaact tcaaagcgtt tccgaaaacg agcgcttccg
     4321 aaaatgcaac gcgagctgcg cacatacagc tcactgttca cgtcgcacct atatctgcgt
     4381 gttgcctgta tatatatata catgagaaga acggcatagt gcgtgtttat gcttaaatgc
     4441 gtacttatat gcgtctattt atgtaggatg aaaggtagtc tagtacctcc tgtgatatta
     4501 tcccattcca tgcggggtat cgtatgcttc cttcagcact accctttagc tgttctatat
     4561 gctgccactc ctcaattgga ttagtctcat ccttcaatgc tatcatttcc tttgatattg
     4621 gatcgatccg atgataagct gtcaaacatg agaattgggt aataactgat ataattaaat
     4681 tgaagctcta atttgtgagt ttagtataca tgcatttact tataatacag ttttttagtt
     4741 ttgctggccg catcttctca aatatgcttc ccagcctgct tttctgtaac gttcaccctc
     4801 taccttagca tcccttccct ttgcaaatag tcctcttcca acaataataa tgtcagatcc
     4861 tgtagagacc acatcatcca cggttctata ctgttgaccc aatgcgtctc ccttgtcatc
     4921 taaacccaca ccgggtgtca taatcaacca atcgtaacct tcatctcttc cacccatgtc
     4981 tctttgagca ataaagccga taacaaaatc tttgtcgctc ttcgcaatgt caacagtacc
     5041 cttagtatat tctccagtag atagggagcc cttgcatgac aattctgcta acatcaaaag
     5101 gcctctaggt tcctttgtta cttcttctgc cgcctgcttc aaaccgctaa caatacctgg
     5161 gcccaccaca ccgtgtgcat tcgtaatgtc tgcccattct gctattctgt atacacccgc
     5221 agagtactgc aatttgactg tattaccaat gtcagcaaat tttctgtctt cgaagagtaa
     5281 aaaattgtac ttggcggata atgcctttag cggcttaact gtgccctcca tggaaaaatc
     5341 agtcaagata tccacatgtg tttttagtaa acaaattttg ggacctaatg cttcaactaa
     5401 ctccagtaat tccttggtgg tacgaacatc caatgaagca cacaagtttg tttgcttttc
     5461 gtgcatgata ttaaatagct tggcagcaac aggactagga tgagtagcag cacgttcctt
     5521 atatgtagct ttcgacatga tttatcttcg tttcctgcat gtttttgttc tgtgcagttg
     5581 ggttaagaat actgggcaat ttcatgtttc ttcaacacta catatgcgta tatataccaa
     5641 tctaagtctg tgctccttcc ttcgttcttc cttctgttcg gagattaccg aatcaaaaaa
     5701 atttcaagga aaccgaaatc aaaaaaaaga ataaaaaaaa aatgatgaat tgaaaagcta
     5761 attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     5821 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     5881 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     5941 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     6001 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     6061 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     6121 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     6181 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     6241 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     6301 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     6361 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     6421 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     6481 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     6541 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     6601 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     6661 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     6721 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     6781 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     6841 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     6901 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     6961 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     7021 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     7081 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     7141 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     7201 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     7261 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     7321 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     7381 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     7441 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     7501 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     7561 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     7621 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     7681 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     7741 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     7801 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     7861 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     7921 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     7981 acagctatga ccatgattac gccaagctta ccgcatcagg aaattgtaag cgttaatatt
     8041 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
     8101 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
     8161 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
     8221 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
     8281 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
     8341 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
     8401 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
     8461 ccgctacagg gcgcgtccat tcgc
//
