LOCUS       sequence-236421-        9934 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  piggyBac-based expression of the tamoxifen-inducible Cre.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9934)
  AUTHORS   Adachi K, Kopp W, Wu G, Heising S, Greber B, Stehling M, 
            Arauzo-Bravo MJ, Boerno ST, Timmermann B, Vingron M, Scholer HR
  TITLE     Esrrb Unlocks Silenced Enhancers for Reprogramming to Naive 
            Pluripotency.
  JOURNAL   Cell Stem Cell. 2018 Aug 2;23(2):266-275.e6. doi: 
            10.1016/j.stem.2018.05.020. Epub 2018 Jun 14.
  PUBMED    29910149
REFERENCE   2  (bases 1 to 9934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2018.05.020"; journalName: "Cell Stem Cell"; date: 
            "2018-08-2- 2"; volume: "23"; issue: "2"; pages: "266-275.e6"
COMMENT     Sequence Label: KA1158_pPBCAG-MerCreMer-IZ
FEATURES             Location/Qualifiers
     source          1..9934
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     623..642
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   660..694
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        972..1355
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1360..1637
                     /label=chicken beta-actin promoter
     intron          1638..2654
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2578..2600
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2662..2681
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             3737..3757
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3758..4783
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="SNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLL
                     SVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLP
                     RPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFL
                     GIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVER
                     WISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQR
                     YLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLED
                     GD"
     misc_feature    join(5819..6393,6394..6396,6397..6405)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5986..6003)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6213..6232
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6406..6780
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     primer_bind     complement(6838..6857)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6903..6958
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6957..6976)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7319..7335)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7343..7359
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7367..7373,7374..7391,7392..7397))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7412..7433
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     repeat_region   7660..7722
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(7746..7764)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7785..7801)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7809..7825
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7833..7839,7840..7857,7858..7863))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7878..7899
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8016..8033)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8187..8775)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8267..8286)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8946..9737,9738..9806))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9569..9588
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9807..9911)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtaccttaac
      661 cctagaaaga tagtctgcgt aaaattgacg catgcattct tgaaatattg ctctctcttt
      721 ctaaatagcg cgaatccgtc gctgtgcatt taggacatct cagtcgccgc ttggagctcc
      781 cgtgaggcgt gcttgtcaat gcggtaagtg tcactgattt tgaactataa cgaccgcgtg
      841 agtcaaaatg acgcatgatt atcttttacg tgacttttaa gatttaactc atacgataat
      901 tatattgtta tttcatgttc tacttacgtg ataacttatt atatatatat tttcttgtta
      961 tagatatcgt cgacattgat tattgactag ctagttatta atagtaatca attacggggt
     1021 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
     1081 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
     1141 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
     1201 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg
     1261 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc
     1321 agtacatcta cgtattagtc atcgctatta ccatgatggt cgaggtgagc cccacgttct
     1381 gcttcactct ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt
     1441 aattattttg tgcagcgatg ggggcggggg gggggggggg gcgcgcgcca ggcggggcgg
     1501 ggcggggcga ggggcggggc ggggcgaggc ggagaggtgc ggcggcagcc aatcagagcg
     1561 gcgcgctccg aaagtttcct tttatggcga ggcggcggcg gcggcggccc tataaaaagc
     1621 gaagcgcgcg gcgggcggga gtcgctgcgc gctgccttcg ccccgtgccc cgctccgccg
     1681 ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt actcccacag gtgagcgggc
     1741 gggacggccc ttctcctccg ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt
     1801 ttctgtggct gcgtgaaagc cttgaggggc tccgggaggg ccctttgtgc ggggggagcg
     1861 gctcgggggg tgcgtgcgtg tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc
     1921 ccggcggctg tgagcgctgc gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg
     1981 aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag
     2041 gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc
     2101 tgcaaccccc cctgcacccc cctccccgag ttgctgagca cggcccggct tcgggtgcgg
     2161 ggctccgtac ggggcgtggc gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg
     2221 gggtgccggg cggggcgggg ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg
     2281 gcccccggag cgccggcggc tgtcgaggcg cggcgagccg cagccattgc cttttatggt
     2341 aatcgtgcga gagggcgcag ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg
     2401 ggaggcgccg ccgcaccccc tctagcgggc gcggggcgaa gcggtgcggc gccggcagga
     2461 aggaaatggg cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc
     2521 agcctcgggg ctgtccgcgg ggggacggct gccttcgggg gggacggggc agggcggggt
     2581 tcggcttctg gcgtgtgacc ggcggctcta gagcctctgc taaccatgtt catgccttct
     2641 tctttttcct acagctcctg ggcaacgtgc tggttattgt gctgtctcat cattttggca
     2701 aagaattcct cgagatctca cgcgtgacta gcttgcatgc ctgcacacca tgggagatcc
     2761 acgaaatgaa atgggtgctt caggagacat gagggctgcc aacctttggc caagccctct
     2821 tgtgattaag cacactaaga agaatagccc tgccttgtcc ttgacagctg accagatggt
     2881 cagtgccttg ttggatgctg aaccgcccat gatctattct gaatatgatc cttctagacc
     2941 cttcagtgaa gcctcaatga tgggcttatt gaccaaccta gcagataggg agctggttca
     3001 tatgatcaac tgggcaaaga gagtgccagg ctttggggac ttgaatctcc atgatcaggt
     3061 ccaccttctc gagtgtgcct ggctggagat tctgatgatt ggtctcgtct ggcgctccat
     3121 ggaacacccg gggaagctcc tgtttgctcc taacttgctc ctggacagga atcaaggtaa
     3181 atgtgtggaa ggcatggtgg agatctttga catgttgctt gctacgtcaa gtcggttccg
     3241 catgatgaac ctgcagggtg aagagtttgt gtgcctcaaa tccatcattt tgcttaattc
     3301 cggagtgtac acgtttctgt ccagcacctt gaagtctctg gaagagaagg accacatcca
     3361 ccgtgtcctg gacaagatca cagacacttt gatccacctg atggccaaag ctggcctgac
     3421 tctgcagcag cagcatcgcc gcctagctca gctccttctc attctttccc atatccggca
     3481 tatgagtaac aaacgcatgg agcatctcta caacatgaaa tgcaagaacg tggtacccct
     3541 ctatgacctg ctcctggaga tgttggatgc ccaccgcctt catgccccag ccagtcgcat
     3601 gggagtgccc ccagaggagc ccagccagac ccagctggcc accaccagct ccacttcagc
     3661 acattcctta caaacctact acataccccc ggaagcagag ggcttcccca acacgatcgt
     3721 cgacctgcag accatgccca agaagaagag gaaggtgtcc aatttactga ccgtacacca
     3781 aaatttgcct gcattaccgg tcgatgcaac gagtgatgag gttcgcaaga acctgatgga
     3841 catgttcagg gatcgccagg cgttttctga gcatacctgg aaaatgcttc tgtccgtttg
     3901 ccggtcgtgg gcggcatggt gcaagttgaa taaccggaaa tggtttcccg cagaacctga
     3961 agatgttcgc gattatcttc tatatcttca ggcgcgcggt ctggcagtaa aaactatcca
     4021 gcaacatttg ggccagctaa acatgcttca tcgtcggtcc gggctgccac gaccaagtga
     4081 cagcaatgct gtttcactgg ttatgcggcg gatccgaaaa gaaaacgttg atgccggtga
     4141 acgtgcaaaa caggctctag cgttcgaacg cactgatttc gaccaggttc gttcactcat
     4201 ggaaaatagc gatcgctgcc aggatatacg taatctggca tttctgggga ttgcttataa
     4261 caccctgtta cgtatagccg aaattgccag gatcagggtt aaagatatct cacgtactga
     4321 cggtgggaga atgttaatcc atattggcag aacgaaaacg ctggttagca ccgcaggtgt
     4381 agagaaggca cttagcctgg gggtaactaa actggtcgag cgatggattt ccgtctctgg
     4441 tgtagctgat gatccgaata actacctgtt ttgccgggtc agaaaaaatg gtgttgccgc
     4501 gccatctgcc accagccagc tatcaactcg cgccctggaa gggatttttg aagcaactca
     4561 tcgattgatt tacggcgcta aggatgactc tggtcagaga tacctggcct ggtctggaca
     4621 cagtgcccgt gtcggagccg cgcgagatat ggcccgcgct ggagtttcaa taccggagat
     4681 catgcaagct ggtggctgga ccaatgtaaa tattgtcatg aactatatcc gtaacctgga
     4741 tagtgaaaca ggggcaatgg tgcgcctgct ggaagatggc gatggaggtt ctggagatcc
     4801 acgaaatgaa atgggtgctt caggagacat gagggctgcc aacctttggc caagccctct
     4861 tgtgattaag cacactaaga agaatagccc tgccttgtcc ttgacagctg accagatggt
     4921 cagtgccttg ttggatgctg aaccgcccat gatctattct gaatatgatc cttctagacc
     4981 cttcagtgaa gcctcaatga tgggcttatt gaccaaccta gcagataggg agctggttca
     5041 tatgatcaac tgggcaaaga gagtgccagg ctttggggac ttgaatctcc atgatcaggt
     5101 ccaccttctc gagtgtgcct ggctggagat tctgatgatt ggtctcgtct ggcgctccat
     5161 ggaacacccg gggaagctcc tgtttgctcc taacttgctc ctggacagga atcaaggtaa
     5221 atgtgtggaa ggcatggtgg agatctttga catgttgctt gctacgtcaa gtcggttccg
     5281 catgatgaac ctgcagggtg aagagtttgt gtgcctcaaa tccatcattt tgcttaattc
     5341 cggagtgtac acgtttctgt ccagcacctt gaagtctctg gaagagaagg accacatcca
     5401 ccgtgtcctg gacaagatca cagacacttt gatccacctg atggccaaag ctggcctgac
     5461 tctgcagcag cagcatcgcc gcctagctca gctccttctc attctttccc atatccggca
     5521 tatgagtaac aaacgcatgg agcatctcta caacatgaaa tgcaaaaacg tggtacccct
     5581 ctatgacctg ctcctggaga tgttggatgc ccaccgcctt catgccccag ccagtcgcat
     5641 gggagtgccc ccagaggagc ccagccagac ccagctggcc accaccagct ccacttcagc
     5701 acattcctta caaacctact acataccccc ggaagcagag ggcttcccca acacgatctg
     5761 agagctccta ctagctgagc ggccgctcga taagcttgat atcgaattcc gccccccccc
     5821 cctctccctc ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg
     5881 cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga
     5941 aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa
     6001 tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa
     6061 caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct
     6121 gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg
     6181 ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg
     6241 ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca
     6301 catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg
     6361 acgtggtttt cctttgaaaa acacgatgat aatatggcca caaccatggc caagttgacc
     6421 agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac
     6481 cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac
     6541 gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg
     6601 gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac
     6661 ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag
     6721 ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggactga
     6781 ccgacgccga ccaacaccgc cggtccgacg cggcccgacg ggtccgaggg gggtcgatct
     6841 ttttccctct gccaaaaatt atggggacat catgaagccc cttgagcatc tgacttctgg
     6901 ctaataaagg aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact
     6961 cggaaggaca tatgggaggg caaatcattt aaaacatcag aatgagtatt tggtttagag
     7021 tttggcaaca tatgcccata tgctggctgc catgaacaaa ggttggctat aaagaggtca
     7081 tcagtatatg aaacagcccc ctgctgtcca ttccttattc catagaaaag ccttgacttg
     7141 aggttagatt ttttttatat tttgttttgt gttatttttt tctttaacat ccctaaaatt
     7201 ttccttacat gttttactag ccagattttt cctcctctcc tgactactcc cagtcatagc
     7261 tgtccctctt ctcttatgga gatccctcga cctgcagccc aagcttggcg taatcatggt
     7321 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     7381 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     7441 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagcggatc ctttgttact
     7501 ttatagaaga aattttgagt ttttgttttt ttttaataaa taaataaaca taaataaatt
     7561 gtttgttgaa tttattatta gtatgtaagt gtaaatataa taaaacttaa tatctattca
     7621 aattaataaa taaacctcga tatacagacc gataaaacac atgcgtcaat tttacgcatg
     7681 attatcttta acgtacgtca caatatgatt atctttctag ggttaaagag ctccagcttt
     7741 tgttcccttt agtgagggtt aatttcgagc ttggcgtaat catggtcata gctgtttcct
     7801 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     7861 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     7921 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     7981 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     8041 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     8101 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     8161 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     8221 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     8281 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     8341 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     8401 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     8461 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     8521 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     8581 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     8641 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     8701 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     8761 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     8821 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     8881 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     8941 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     9001 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     9061 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     9121 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     9181 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     9241 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     9301 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     9361 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     9421 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     9481 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     9541 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     9601 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     9661 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     9721 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     9781 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     9841 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     9901 ggggttccgc gcacatttcc ccgaaaagtg ccac
//
