LOCUS       sequence-236424-        4630 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luciferase reporter driven by the Klf4 enhancer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4630)
  AUTHORS   Adachi K, Kopp W, Wu G, Heising S, Greber B, Stehling M, 
            Arauzo-Bravo MJ, Boerno ST, Timmermann B, Vingron M, Scholer HR
  TITLE     Esrrb Unlocks Silenced Enhancers for Reprogramming to Naive 
            Pluripotency.
  JOURNAL   Cell Stem Cell. 2018 Aug 2;23(2):266-275.e6. doi: 
            10.1016/j.stem.2018.05.020. Epub 2018 Jun 14.
  PUBMED    29910149
REFERENCE   2  (bases 1 to 4630)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2018.05.020"; journalName: "Cell Stem Cell"; date: 
            "2018-08-2- 2"; volume: "23"; issue: "2"; pages: "266-275.e6"
COMMENT     Sequence Label: KA1396_pGL4-UBCmin-luc2/Klf4-E'
FEATURES             Location/Qualifiers
     source          1..4630
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      182..191
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             188..1840
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    1884..2005
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1895..1914)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1949..1968
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2553..2570)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2724..3312)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2804..2823)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3512..4303,4304..4372))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    4477..4525
                     /note="synthetic polyadenylation signal"
     misc_feature    4539..4630
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     4579..4598
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tggggcggtg aacgccgatg
       61 attatataag gacgcgccgg gtgtggcaca gctagttccg tcgcagccgg gatttgaatt
      121 cctcgactct agaggatctg gcctcggcgg ccaagcttgg caatccggta ctgttggtaa
      181 agccaccatg gaagatgcca aaaacattaa gaagggccca gcgccattct acccactcga
      241 agacgggacc gccggcgagc agctgcacaa agccatgaag cgctacgccc tggtgcccgg
      301 caccatcgcc tttaccgacg cacatatcga ggtggacatt acctacgccg agtacttcga
      361 gatgagcgtt cggctggcag aagctatgaa gcgctatggg ctgaatacaa accatcggat
      421 cgtggtgtgc agcgagaata gcttgcagtt cttcatgccc gtgttgggtg ccctgttcat
      481 cggtgtggct gtggccccag ctaacgacat ctacaacgag cgcgagctgc tgaacagcat
      541 gggcatcagc cagcccaccg tcgtattcgt gagcaagaaa gggctgcaaa agatcctcaa
      601 cgtgcaaaag aagctaccga tcatacaaaa gatcatcatc atggatagca agaccgacta
      661 ccagggcttc caaagcatgt acaccttcgt gacttcccat ttgccacccg gcttcaacga
      721 gtacgacttc gtgcccgaga gcttcgaccg ggacaaaacc atcgccctga tcatgaacag
      781 tagtggcagt accggattgc ccaagggcgt agccctaccg caccgcaccg cttgtgtccg
      841 attcagtcat gcccgcgacc ccatcttcgg caaccagatc atccccgaca ccgctatcct
      901 cagcgtggtg ccatttcacc acggcttcgg catgttcacc acgctgggct acttgatctg
      961 cggctttcgg gtcgtgctca tgtaccgctt cgaggaggag ctattcttgc gcagcttgca
     1021 agactataag attcaatctg ccctgctggt gcccacacta tttagcttct tcgctaagag
     1081 cactctcatc gacaagtacg acctaagcaa cttgcacgag atcgccagcg gcggggcgcc
     1141 gctcagcaag gaggtaggtg aggccgtggc caaacgcttc cacctaccag gcatccgcca
     1201 gggctacggc ctgacagaaa caaccagcgc cattctgatc acccccgaag gggacgacaa
     1261 gcctggcgca gtaggcaagg tggtgccctt cttcgaggct aaggtggtgg acttggacac
     1321 cggtaagaca ctgggtgtga accagcgcgg cgagctgtgc gtccgtggcc ccatgatcat
     1381 gagcggctac gttaacaacc ccgaggctac aaacgctctc atcgacaagg acggctggct
     1441 gcacagcggc gacatcgcct actgggacga ggacgagcac ttcttcatcg tggaccggct
     1501 gaagagcctg atcaaataca agggctacca ggtagcccca gccgaactgg agagcatcct
     1561 gctgcaacac cccaacatct tcgacgccgg ggtcgccggc ctgcccgacg acgatgccgg
     1621 cgagctgccc gccgcagtcg tcgtgctgga acacggtaaa accatgaccg agaaggagat
     1681 cgtggactat gtggccagcc aggttacaac cgccaagaag ctgcgcggtg gtgttgtgtt
     1741 cgtggacgag gtgcctaaag gactgaccgg caagttggac gcccgcaaga tccgcgagat
     1801 tctcattaag gccaagaagg gcggcaagat cgccgtgtaa taattctaga gtcggggcgg
     1861 ccggccgctt cgagcagaca tgataagata cattgatgag tttggacaaa ccacaactag
     1921 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
     1981 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt
     2041 tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtaaaat
     2101 cgataaggat ctagtccaat gtcaaattcc ggacaaattc ctagaagtgg cctcaaaagc
     2161 tctccccacg aattaacgac tctgatcttc atataaatgg agaaaccccc gcactttgtt
     2221 ggtcagctgc tttcccttat aataatgtat tcaaggttag caggttatag tccatccttt
     2281 gccaggaagg ttgctcagga ttgaacaggc aaggatgacc tccacacaac ttcattcttt
     2341 ttaaaattct attagggatc caggccacat tttcctgatc cattcatcac cctgtagaca
     2401 cgtgcactac ctgtcgaccg atgcccttga gagccttcaa cccagtcagc tccttccggt
     2461 gggcgcgggg catgactatc gtcgccgcac ttatgactgt cttctttatc atgcaactcg
     2521 taggacaggt gccggcagcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     2581 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     2641 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     2701 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     2761 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     2821 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     2881 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     2941 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     3001 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     3061 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     3121 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     3181 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     3241 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     3301 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     3361 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     3421 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     3481 agcggccgca aatgctaaac cactgcagtg gttaccagtg cttgatcagt gaggcaccga
     3541 tctcagcgat ctgcctattt cgttcgtcca tagtggcctg actccccgtc gtgtagatca
     3601 ctacgattcg tgagggctta ccatcaggcc ccagcgcagc aatgatgccg cgagagccgc
     3661 gttcaccggc ccccgatttg tcagcaatga accagccagc agggagggcc gagcgaagaa
     3721 gtggtcctgc tactttgtcc gcctccatcc agtctatgag ctgctgtcgt gatgctagag
     3781 taagaagttc gccagtgagt agtttccgaa gagttgtggc cattgctact ggcatcgtgg
     3841 tatcacgctc gtcgttcggt atggcttcgt tcaactctgg ttcccagcgg tcaagccggg
     3901 tcacatgatc acccatatta tgaagaaatg cagtcagctc cttagggcct ccgatcgttg
     3961 tcagaagtaa gttggccgcg gtgttgtcgc tcatggtaat ggcagcacta cacaattctc
     4021 ttaccgtcat gccatccgta agatgctttt ccgtgaccgg cgagtactca accaagtcgt
     4081 tttgtgagta gtgtatacgg cgaccaagct gctcttgccc ggcgtctata cgggacaaca
     4141 ccgcgccaca tagcagtact ttgaaagtgc tcatcatcgg gaatcgttct tcggggcgga
     4201 aagactcaag gatcttgccg ctattgagat ccagttcgat atagcccact cttgcaccca
     4261 gttgatcttc agcatctttt actttcacca gcgtttcggg gtgtgcaaaa acaggcaagc
     4321 aaaatgccgc aaagaaggga atgagtgcga cacgaaaatg ttggatgctc atactcgtcc
     4381 tttttcaata ttattgaagc atttatcagg gttactagta cgtctctcaa ggataagtaa
     4441 gtaatattaa ggtacgggag gtattggaca ggccgcaata aaatatcttt attttcatta
     4501 catctgtgtg ttggtttttt gtgtgaatcg atagtactaa catacgctct ccatcaaaac
     4561 aaaacgaaac aaaacaaact agcaaaatag gctgtcccca gtgcaagtgc aggtgccaga
     4621 acatttctct
//
