LOCUS       sequence-236426-        4526 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luciferase reporter driven by the Nanog enhancer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4526)
  AUTHORS   Adachi K, Kopp W, Wu G, Heising S, Greber B, Stehling M, 
            Arauzo-Bravo MJ, Boerno ST, Timmermann B, Vingron M, Scholer HR
  TITLE     Esrrb Unlocks Silenced Enhancers for Reprogramming to Naive 
            Pluripotency.
  JOURNAL   Cell Stem Cell. 2018 Aug 2;23(2):266-275.e6. doi: 
            10.1016/j.stem.2018.05.020. Epub 2018 Jun 14.
  PUBMED    29910149
REFERENCE   2  (bases 1 to 4526)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2018.05.020"; journalName: "Cell Stem Cell"; date: 
            "2018-08-2- 2"; volume: "23"; issue: "2"; pages: "266-275.e6"
COMMENT     Sequence Label: KA1402_pGL4-UBCmin-luc2/Nanog-A'
FEATURES             Location/Qualifiers
     source          1..4526
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      182..191
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             188..1840
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    1884..2005
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1895..1914)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1949..1968
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2449..2466)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2620..3208)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2700..2719)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3408..4199,4200..4268))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    4373..4421
                     /note="synthetic polyadenylation signal"
     misc_feature    4435..4526
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     4475..4494
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tggggcggtg aacgccgatg
       61 attatataag gacgcgccgg gtgtggcaca gctagttccg tcgcagccgg gatttgaatt
      121 cctcgactct agaggatctg gcctcggcgg ccaagcttgg caatccggta ctgttggtaa
      181 agccaccatg gaagatgcca aaaacattaa gaagggccca gcgccattct acccactcga
      241 agacgggacc gccggcgagc agctgcacaa agccatgaag cgctacgccc tggtgcccgg
      301 caccatcgcc tttaccgacg cacatatcga ggtggacatt acctacgccg agtacttcga
      361 gatgagcgtt cggctggcag aagctatgaa gcgctatggg ctgaatacaa accatcggat
      421 cgtggtgtgc agcgagaata gcttgcagtt cttcatgccc gtgttgggtg ccctgttcat
      481 cggtgtggct gtggccccag ctaacgacat ctacaacgag cgcgagctgc tgaacagcat
      541 gggcatcagc cagcccaccg tcgtattcgt gagcaagaaa gggctgcaaa agatcctcaa
      601 cgtgcaaaag aagctaccga tcatacaaaa gatcatcatc atggatagca agaccgacta
      661 ccagggcttc caaagcatgt acaccttcgt gacttcccat ttgccacccg gcttcaacga
      721 gtacgacttc gtgcccgaga gcttcgaccg ggacaaaacc atcgccctga tcatgaacag
      781 tagtggcagt accggattgc ccaagggcgt agccctaccg caccgcaccg cttgtgtccg
      841 attcagtcat gcccgcgacc ccatcttcgg caaccagatc atccccgaca ccgctatcct
      901 cagcgtggtg ccatttcacc acggcttcgg catgttcacc acgctgggct acttgatctg
      961 cggctttcgg gtcgtgctca tgtaccgctt cgaggaggag ctattcttgc gcagcttgca
     1021 agactataag attcaatctg ccctgctggt gcccacacta tttagcttct tcgctaagag
     1081 cactctcatc gacaagtacg acctaagcaa cttgcacgag atcgccagcg gcggggcgcc
     1141 gctcagcaag gaggtaggtg aggccgtggc caaacgcttc cacctaccag gcatccgcca
     1201 gggctacggc ctgacagaaa caaccagcgc cattctgatc acccccgaag gggacgacaa
     1261 gcctggcgca gtaggcaagg tggtgccctt cttcgaggct aaggtggtgg acttggacac
     1321 cggtaagaca ctgggtgtga accagcgcgg cgagctgtgc gtccgtggcc ccatgatcat
     1381 gagcggctac gttaacaacc ccgaggctac aaacgctctc atcgacaagg acggctggct
     1441 gcacagcggc gacatcgcct actgggacga ggacgagcac ttcttcatcg tggaccggct
     1501 gaagagcctg atcaaataca agggctacca ggtagcccca gccgaactgg agagcatcct
     1561 gctgcaacac cccaacatct tcgacgccgg ggtcgccggc ctgcccgacg acgatgccgg
     1621 cgagctgccc gccgcagtcg tcgtgctgga acacggtaaa accatgaccg agaaggagat
     1681 cgtggactat gtggccagcc aggttacaac cgccaagaag ctgcgcggtg gtgttgtgtt
     1741 cgtggacgag gtgcctaaag gactgaccgg caagttggac gcccgcaaga tccgcgagat
     1801 tctcattaag gccaagaagg gcggcaagat cgccgtgtaa taattctaga gtcggggcgg
     1861 ccggccgctt cgagcagaca tgataagata cattgatgag tttggacaaa ccacaactag
     1921 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
     1981 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt
     2041 tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtaaaat
     2101 cgataaggat ccagagatcg ccagggtctg gaggtgcagc cgtggttaaa agatgaataa
     2161 agtgaaatga ggtaaagcct ctttttgggg gggggggatg tctttagatc agaggatgcc
     2221 ccctaagctt tccctccctc ccagtctggg tcaccttaca gcttcttttg cattacaatg
     2281 tccatggtgg accctgcagg tgggattagt cgaccgatgc ccttgagagc cttcaaccca
     2341 gtcagctcct tccggtgggc gcggggcatg actatcgtcg ccgcacttat gactgtcttc
     2401 tttatcatgc aactcgtagg acaggtgccg gcagcgctct tccgcttcct cgctcactga
     2461 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     2521 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     2581 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     2641 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     2701 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     2761 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     2821 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     2881 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     2941 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     3001 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     3061 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     3121 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     3181 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     3241 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     3301 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     3361 gtaaacttgg tctgacagcg gccgcaaatg ctaaaccact gcagtggtta ccagtgcttg
     3421 atcagtgagg caccgatctc agcgatctgc ctatttcgtt cgtccatagt ggcctgactc
     3481 cccgtcgtgt agatcactac gattcgtgag ggcttaccat caggccccag cgcagcaatg
     3541 atgccgcgag agccgcgttc accggccccc gatttgtcag caatgaacca gccagcaggg
     3601 agggccgagc gaagaagtgg tcctgctact ttgtccgcct ccatccagtc tatgagctgc
     3661 tgtcgtgatg ctagagtaag aagttcgcca gtgagtagtt tccgaagagt tgtggccatt
     3721 gctactggca tcgtggtatc acgctcgtcg ttcggtatgg cttcgttcaa ctctggttcc
     3781 cagcggtcaa gccgggtcac atgatcaccc atattatgaa gaaatgcagt cagctcctta
     3841 gggcctccga tcgttgtcag aagtaagttg gccgcggtgt tgtcgctcat ggtaatggca
     3901 gcactacaca attctcttac cgtcatgcca tccgtaagat gcttttccgt gaccggcgag
     3961 tactcaacca agtcgttttg tgagtagtgt atacggcgac caagctgctc ttgcccggcg
     4021 tctatacggg acaacaccgc gccacatagc agtactttga aagtgctcat catcgggaat
     4081 cgttcttcgg ggcggaaaga ctcaaggatc ttgccgctat tgagatccag ttcgatatag
     4141 cccactcttg cacccagttg atcttcagca tcttttactt tcaccagcgt ttcggggtgt
     4201 gcaaaaacag gcaagcaaaa tgccgcaaag aagggaatga gtgcgacacg aaaatgttgg
     4261 atgctcatac tcgtcctttt tcaatattat tgaagcattt atcagggtta ctagtacgtc
     4321 tctcaaggat aagtaagtaa tattaaggta cgggaggtat tggacaggcc gcaataaaat
     4381 atctttattt tcattacatc tgtgtgttgg ttttttgtgt gaatcgatag tactaacata
     4441 cgctctccat caaaacaaaa cgaaacaaaa caaactagca aaataggctg tccccagtgc
     4501 aagtgcaggt gccagaacat ttctct
//
