LOCUS       sequence-236427-        5016 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luciferase reporter driven by the Nanog enhancer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5016)
  AUTHORS   Adachi K, Kopp W, Wu G, Heising S, Greber B, Stehling M, 
            Arauzo-Bravo MJ, Boerno ST, Timmermann B, Vingron M, Scholer HR
  TITLE     Esrrb Unlocks Silenced Enhancers for Reprogramming to Naive 
            Pluripotency.
  JOURNAL   Cell Stem Cell. 2018 Aug 2;23(2):266-275.e6. doi: 
            10.1016/j.stem.2018.05.020. Epub 2018 Jun 14.
  PUBMED    29910149
REFERENCE   2  (bases 1 to 5016)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2018.05.020"; journalName: "Cell Stem Cell"; date: 
            "2018-08-2- 2"; volume: "23"; issue: "2"; pages: "266-275.e6"
COMMENT     Sequence Label: KA1403_pGL4-UBCmin-luc2/Nanog-B'
FEATURES             Location/Qualifiers
     source          1..5016
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      182..191
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             188..1840
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    1884..2005
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1895..1914)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1949..1968
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2939..2956)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3110..3698)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3190..3209)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3898..4689,4690..4758))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    4863..4911
                     /note="synthetic polyadenylation signal"
     misc_feature    4925..5016
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     4965..4984
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tggggcggtg aacgccgatg
       61 attatataag gacgcgccgg gtgtggcaca gctagttccg tcgcagccgg gatttgaatt
      121 cctcgactct agaggatctg gcctcggcgg ccaagcttgg caatccggta ctgttggtaa
      181 agccaccatg gaagatgcca aaaacattaa gaagggccca gcgccattct acccactcga
      241 agacgggacc gccggcgagc agctgcacaa agccatgaag cgctacgccc tggtgcccgg
      301 caccatcgcc tttaccgacg cacatatcga ggtggacatt acctacgccg agtacttcga
      361 gatgagcgtt cggctggcag aagctatgaa gcgctatggg ctgaatacaa accatcggat
      421 cgtggtgtgc agcgagaata gcttgcagtt cttcatgccc gtgttgggtg ccctgttcat
      481 cggtgtggct gtggccccag ctaacgacat ctacaacgag cgcgagctgc tgaacagcat
      541 gggcatcagc cagcccaccg tcgtattcgt gagcaagaaa gggctgcaaa agatcctcaa
      601 cgtgcaaaag aagctaccga tcatacaaaa gatcatcatc atggatagca agaccgacta
      661 ccagggcttc caaagcatgt acaccttcgt gacttcccat ttgccacccg gcttcaacga
      721 gtacgacttc gtgcccgaga gcttcgaccg ggacaaaacc atcgccctga tcatgaacag
      781 tagtggcagt accggattgc ccaagggcgt agccctaccg caccgcaccg cttgtgtccg
      841 attcagtcat gcccgcgacc ccatcttcgg caaccagatc atccccgaca ccgctatcct
      901 cagcgtggtg ccatttcacc acggcttcgg catgttcacc acgctgggct acttgatctg
      961 cggctttcgg gtcgtgctca tgtaccgctt cgaggaggag ctattcttgc gcagcttgca
     1021 agactataag attcaatctg ccctgctggt gcccacacta tttagcttct tcgctaagag
     1081 cactctcatc gacaagtacg acctaagcaa cttgcacgag atcgccagcg gcggggcgcc
     1141 gctcagcaag gaggtaggtg aggccgtggc caaacgcttc cacctaccag gcatccgcca
     1201 gggctacggc ctgacagaaa caaccagcgc cattctgatc acccccgaag gggacgacaa
     1261 gcctggcgca gtaggcaagg tggtgccctt cttcgaggct aaggtggtgg acttggacac
     1321 cggtaagaca ctgggtgtga accagcgcgg cgagctgtgc gtccgtggcc ccatgatcat
     1381 gagcggctac gttaacaacc ccgaggctac aaacgctctc atcgacaagg acggctggct
     1441 gcacagcggc gacatcgcct actgggacga ggacgagcac ttcttcatcg tggaccggct
     1501 gaagagcctg atcaaataca agggctacca ggtagcccca gccgaactgg agagcatcct
     1561 gctgcaacac cccaacatct tcgacgccgg ggtcgccggc ctgcccgacg acgatgccgg
     1621 cgagctgccc gccgcagtcg tcgtgctgga acacggtaaa accatgaccg agaaggagat
     1681 cgtggactat gtggccagcc aggttacaac cgccaagaag ctgcgcggtg gtgttgtgtt
     1741 cgtggacgag gtgcctaaag gactgaccgg caagttggac gcccgcaaga tccgcgagat
     1801 tctcattaag gccaagaagg gcggcaagat cgccgtgtaa taattctaga gtcggggcgg
     1861 ccggccgctt cgagcagaca tgataagata cattgatgag tttggacaaa ccacaactag
     1921 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
     1981 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt
     2041 tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtaaaat
     2101 cgataaggat ccccctagtc cccgctcctt ttcagcacta accatacaag ttcatctttt
     2161 actcttgttt tgactgctaa ccacccagag gacccactta acattccttt ccccacccac
     2221 acagctagtt ccaaccaggt ctaaggcatc tagacgccct cctccctatt caaaccgctt
     2281 ccctggataa ggaatgtagc aagcctgcct tttggctacc agccacctct tcgctcggat
     2341 ctttcaccag agactctcaa agacactaaa gaggcaggac aggaatgggg gttggggagg
     2401 gatccatcgc cgtctcctaa gcagactcct ttgacccgga gctgtgcgcc ctgtaccaaa
     2461 cctttgtaga acttggggta aacttaaggc tatggtggcc ttgactccgt ggacccagag
     2521 gcaagtttcc tcctttagag gactcgcatg cattttgttt ctaatttgaa atgagaaccg
     2581 gcttagagct tgaaccagcc agttctctgg actcctccca gctcttacaa ttcctctccc
     2641 ggacggttcc tagaagacaa aggcaagctt accaaaatta cgtcgccctt gggacacacc
     2701 tagggttccc tggtggcatc tttttttttt cattataaac aggagtaaat ttttgtaagg
     2761 gcagagctgg tagctgaggg agaggaaccc tttggcctgt cgaccgatgc ccttgagagc
     2821 cttcaaccca gtcagctcct tccggtgggc gcggggcatg actatcgtcg ccgcacttat
     2881 gactgtcttc tttatcatgc aactcgtagg acaggtgccg gcagcgctct tccgcttcct
     2941 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     3001 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     3061 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     3121 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     3181 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     3241 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     3301 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     3361 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     3421 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     3481 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     3541 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     3601 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     3661 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     3721 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     3781 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     3841 gtatatatga gtaaacttgg tctgacagcg gccgcaaatg ctaaaccact gcagtggtta
     3901 ccagtgcttg atcagtgagg caccgatctc agcgatctgc ctatttcgtt cgtccatagt
     3961 ggcctgactc cccgtcgtgt agatcactac gattcgtgag ggcttaccat caggccccag
     4021 cgcagcaatg atgccgcgag agccgcgttc accggccccc gatttgtcag caatgaacca
     4081 gccagcaggg agggccgagc gaagaagtgg tcctgctact ttgtccgcct ccatccagtc
     4141 tatgagctgc tgtcgtgatg ctagagtaag aagttcgcca gtgagtagtt tccgaagagt
     4201 tgtggccatt gctactggca tcgtggtatc acgctcgtcg ttcggtatgg cttcgttcaa
     4261 ctctggttcc cagcggtcaa gccgggtcac atgatcaccc atattatgaa gaaatgcagt
     4321 cagctcctta gggcctccga tcgttgtcag aagtaagttg gccgcggtgt tgtcgctcat
     4381 ggtaatggca gcactacaca attctcttac cgtcatgcca tccgtaagat gcttttccgt
     4441 gaccggcgag tactcaacca agtcgttttg tgagtagtgt atacggcgac caagctgctc
     4501 ttgcccggcg tctatacggg acaacaccgc gccacatagc agtactttga aagtgctcat
     4561 catcgggaat cgttcttcgg ggcggaaaga ctcaaggatc ttgccgctat tgagatccag
     4621 ttcgatatag cccactcttg cacccagttg atcttcagca tcttttactt tcaccagcgt
     4681 ttcggggtgt gcaaaaacag gcaagcaaaa tgccgcaaag aagggaatga gtgcgacacg
     4741 aaaatgttgg atgctcatac tcgtcctttt tcaatattat tgaagcattt atcagggtta
     4801 ctagtacgtc tctcaaggat aagtaagtaa tattaaggta cgggaggtat tggacaggcc
     4861 gcaataaaat atctttattt tcattacatc tgtgtgttgg ttttttgtgt gaatcgatag
     4921 tactaacata cgctctccat caaaacaaaa cgaaacaaaa caaactagca aaataggctg
     4981 tccccagtgc aagtgcaggt gccagaacat ttctct
//
