LOCUS       sequence-236428-        4769 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luciferase reporter driven by the Pou5f1 enhancer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4769)
  AUTHORS   Adachi K, Kopp W, Wu G, Heising S, Greber B, Stehling M, 
            Arauzo-Bravo MJ, Boerno ST, Timmermann B, Vingron M, Scholer HR
  TITLE     Esrrb Unlocks Silenced Enhancers for Reprogramming to Naive 
            Pluripotency.
  JOURNAL   Cell Stem Cell. 2018 Aug 2;23(2):266-275.e6. doi: 
            10.1016/j.stem.2018.05.020. Epub 2018 Jun 14.
  PUBMED    29910149
REFERENCE   2  (bases 1 to 4769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2018.05.020"; journalName: "Cell Stem Cell"; date: 
            "2018-08-2- 2"; volume: "23"; issue: "2"; pages: "266-275.e6"
COMMENT     Sequence Label: KA1865_pGL4-UBCmin-luc2/Pou5f1_CR4
FEATURES             Location/Qualifiers
     source          1..4769
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      182..191
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             188..1840
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    1884..2005
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1895..1914)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1949..1968
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2692..2709)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2863..3451)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2943..2962)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3651..4442,4443..4511))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    4616..4664
                     /note="synthetic polyadenylation signal"
     misc_feature    4678..4769
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     4718..4737
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tggggcggtg aacgccgatg
       61 attatataag gacgcgccgg gtgtggcaca gctagttccg tcgcagccgg gatttgaatt
      121 cctcgactct agaggatctg gcctcggcgg ccaagcttgg caatccggta ctgttggtaa
      181 agccaccatg gaagatgcca aaaacattaa gaagggccca gcgccattct acccactcga
      241 agacgggacc gccggcgagc agctgcacaa agccatgaag cgctacgccc tggtgcccgg
      301 caccatcgcc tttaccgacg cacatatcga ggtggacatt acctacgccg agtacttcga
      361 gatgagcgtt cggctggcag aagctatgaa gcgctatggg ctgaatacaa accatcggat
      421 cgtggtgtgc agcgagaata gcttgcagtt cttcatgccc gtgttgggtg ccctgttcat
      481 cggtgtggct gtggccccag ctaacgacat ctacaacgag cgcgagctgc tgaacagcat
      541 gggcatcagc cagcccaccg tcgtattcgt gagcaagaaa gggctgcaaa agatcctcaa
      601 cgtgcaaaag aagctaccga tcatacaaaa gatcatcatc atggatagca agaccgacta
      661 ccagggcttc caaagcatgt acaccttcgt gacttcccat ttgccacccg gcttcaacga
      721 gtacgacttc gtgcccgaga gcttcgaccg ggacaaaacc atcgccctga tcatgaacag
      781 tagtggcagt accggattgc ccaagggcgt agccctaccg caccgcaccg cttgtgtccg
      841 attcagtcat gcccgcgacc ccatcttcgg caaccagatc atccccgaca ccgctatcct
      901 cagcgtggtg ccatttcacc acggcttcgg catgttcacc acgctgggct acttgatctg
      961 cggctttcgg gtcgtgctca tgtaccgctt cgaggaggag ctattcttgc gcagcttgca
     1021 agactataag attcaatctg ccctgctggt gcccacacta tttagcttct tcgctaagag
     1081 cactctcatc gacaagtacg acctaagcaa cttgcacgag atcgccagcg gcggggcgcc
     1141 gctcagcaag gaggtaggtg aggccgtggc caaacgcttc cacctaccag gcatccgcca
     1201 gggctacggc ctgacagaaa caaccagcgc cattctgatc acccccgaag gggacgacaa
     1261 gcctggcgca gtaggcaagg tggtgccctt cttcgaggct aaggtggtgg acttggacac
     1321 cggtaagaca ctgggtgtga accagcgcgg cgagctgtgc gtccgtggcc ccatgatcat
     1381 gagcggctac gttaacaacc ccgaggctac aaacgctctc atcgacaagg acggctggct
     1441 gcacagcggc gacatcgcct actgggacga ggacgagcac ttcttcatcg tggaccggct
     1501 gaagagcctg atcaaataca agggctacca ggtagcccca gccgaactgg agagcatcct
     1561 gctgcaacac cccaacatct tcgacgccgg ggtcgccggc ctgcccgacg acgatgccgg
     1621 cgagctgccc gccgcagtcg tcgtgctgga acacggtaaa accatgaccg agaaggagat
     1681 cgtggactat gtggccagcc aggttacaac cgccaagaag ctgcgcggtg gtgttgtgtt
     1741 cgtggacgag gtgcctaaag gactgaccgg caagttggac gcccgcaaga tccgcgagat
     1801 tctcattaag gccaagaagg gcggcaagat cgccgtgtaa taattctaga gtcggggcgg
     1861 ccggccgctt cgagcagaca tgataagata cattgatgag tttggacaaa ccacaactag
     1921 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
     1981 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt
     2041 tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtaaaat
     2101 cgataaggat ccttgtcctg gccagtgagt caccaaaaga gaaatcacaa tccataagac
     2161 aaggttggta ttgaatacag acaggactgc tgggctgcag gcatacttga actgtggtgg
     2221 agagtgctgt ctaggcctta gaggctggcc ctgggaggaa ctgggtgtgg ggaggttgta
     2281 gcccgaccct gcccctcccc ccagggaggt tgagagttct gggcagacgg cagatgcata
     2341 acaaaggtgc atgatagctc tgccctgggg gcagagaaga tggttgggga ggggtccctc
     2401 tcgtcctagc ccttccttaa tctgctattg aggaagcttt gtgaacttgg cggcttccaa
     2461 gtcgctgcct ttatttaggt cttccaacta acctatggca ctgttccaca atgaatgtat
     2521 agaaattggg aggtgagcat gacagagtgg agtcgaccga tgcccttgag agccttcaac
     2581 ccagtcagct ccttccggtg ggcgcggggc atgactatcg tcgccgcact tatgactgtc
     2641 ttctttatca tgcaactcgt aggacaggtg ccggcagcgc tcttccgctt cctcgctcac
     2701 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     2761 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     2821 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     2881 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     2941 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     3001 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     3061 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     3121 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     3181 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     3241 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     3301 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     3361 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     3421 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     3481 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     3541 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     3601 tgagtaaact tggtctgaca gcggccgcaa atgctaaacc actgcagtgg ttaccagtgc
     3661 ttgatcagtg aggcaccgat ctcagcgatc tgcctatttc gttcgtccat agtggcctga
     3721 ctccccgtcg tgtagatcac tacgattcgt gagggcttac catcaggccc cagcgcagca
     3781 atgatgccgc gagagccgcg ttcaccggcc cccgatttgt cagcaatgaa ccagccagca
     3841 gggagggccg agcgaagaag tggtcctgct actttgtccg cctccatcca gtctatgagc
     3901 tgctgtcgtg atgctagagt aagaagttcg ccagtgagta gtttccgaag agttgtggcc
     3961 attgctactg gcatcgtggt atcacgctcg tcgttcggta tggcttcgtt caactctggt
     4021 tcccagcggt caagccgggt cacatgatca cccatattat gaagaaatgc agtcagctcc
     4081 ttagggcctc cgatcgttgt cagaagtaag ttggccgcgg tgttgtcgct catggtaatg
     4141 gcagcactac acaattctct taccgtcatg ccatccgtaa gatgcttttc cgtgaccggc
     4201 gagtactcaa ccaagtcgtt ttgtgagtag tgtatacggc gaccaagctg ctcttgcccg
     4261 gcgtctatac gggacaacac cgcgccacat agcagtactt tgaaagtgct catcatcggg
     4321 aatcgttctt cggggcggaa agactcaagg atcttgccgc tattgagatc cagttcgata
     4381 tagcccactc ttgcacccag ttgatcttca gcatctttta ctttcaccag cgtttcgggg
     4441 tgtgcaaaaa caggcaagca aaatgccgca aagaagggaa tgagtgcgac acgaaaatgt
     4501 tggatgctca tactcgtcct ttttcaatat tattgaagca tttatcaggg ttactagtac
     4561 gtctctcaag gataagtaag taatattaag gtacgggagg tattggacag gccgcaataa
     4621 aatatcttta ttttcattac atctgtgtgt tggttttttg tgtgaatcga tagtactaac
     4681 atacgctctc catcaaaaca aaacgaaaca aaacaaacta gcaaaatagg ctgtccccag
     4741 tgcaagtgca ggtgccagaa catttctct
//
