LOCUS       sequence-236433-        4808 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luciferase reporter driven by the Esrrb enhancer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4808)
  AUTHORS   Adachi K, Kopp W, Wu G, Heising S, Greber B, Stehling M, 
            Arauzo-Bravo MJ, Boerno ST, Timmermann B, Vingron M, Scholer HR
  TITLE     Esrrb Unlocks Silenced Enhancers for Reprogramming to Naive 
            Pluripotency.
  JOURNAL   Cell Stem Cell. 2018 Aug 2;23(2):266-275.e6. doi: 
            10.1016/j.stem.2018.05.020. Epub 2018 Jun 14.
  PUBMED    29910149
REFERENCE   2  (bases 1 to 4808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2018.05.020"; journalName: "Cell Stem Cell"; date: 
            "2018-08-2- 2"; volume: "23"; issue: "2"; pages: "266-275.e6"
COMMENT     Sequence Label: KA1870_pGL4-UBCmin-luc2/Esrrb-B'
FEATURES             Location/Qualifiers
     source          1..4808
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      182..191
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             188..1840
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    1884..2005
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1895..1914)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1949..1968
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2731..2748)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2902..3490)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2982..3001)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3690..4481,4482..4550))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    4655..4703
                     /note="synthetic polyadenylation signal"
     misc_feature    4717..4808
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     4757..4776
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tggggcggtg aacgccgatg
       61 attatataag gacgcgccgg gtgtggcaca gctagttccg tcgcagccgg gatttgaatt
      121 cctcgactct agaggatctg gcctcggcgg ccaagcttgg caatccggta ctgttggtaa
      181 agccaccatg gaagatgcca aaaacattaa gaagggccca gcgccattct acccactcga
      241 agacgggacc gccggcgagc agctgcacaa agccatgaag cgctacgccc tggtgcccgg
      301 caccatcgcc tttaccgacg cacatatcga ggtggacatt acctacgccg agtacttcga
      361 gatgagcgtt cggctggcag aagctatgaa gcgctatggg ctgaatacaa accatcggat
      421 cgtggtgtgc agcgagaata gcttgcagtt cttcatgccc gtgttgggtg ccctgttcat
      481 cggtgtggct gtggccccag ctaacgacat ctacaacgag cgcgagctgc tgaacagcat
      541 gggcatcagc cagcccaccg tcgtattcgt gagcaagaaa gggctgcaaa agatcctcaa
      601 cgtgcaaaag aagctaccga tcatacaaaa gatcatcatc atggatagca agaccgacta
      661 ccagggcttc caaagcatgt acaccttcgt gacttcccat ttgccacccg gcttcaacga
      721 gtacgacttc gtgcccgaga gcttcgaccg ggacaaaacc atcgccctga tcatgaacag
      781 tagtggcagt accggattgc ccaagggcgt agccctaccg caccgcaccg cttgtgtccg
      841 attcagtcat gcccgcgacc ccatcttcgg caaccagatc atccccgaca ccgctatcct
      901 cagcgtggtg ccatttcacc acggcttcgg catgttcacc acgctgggct acttgatctg
      961 cggctttcgg gtcgtgctca tgtaccgctt cgaggaggag ctattcttgc gcagcttgca
     1021 agactataag attcaatctg ccctgctggt gcccacacta tttagcttct tcgctaagag
     1081 cactctcatc gacaagtacg acctaagcaa cttgcacgag atcgccagcg gcggggcgcc
     1141 gctcagcaag gaggtaggtg aggccgtggc caaacgcttc cacctaccag gcatccgcca
     1201 gggctacggc ctgacagaaa caaccagcgc cattctgatc acccccgaag gggacgacaa
     1261 gcctggcgca gtaggcaagg tggtgccctt cttcgaggct aaggtggtgg acttggacac
     1321 cggtaagaca ctgggtgtga accagcgcgg cgagctgtgc gtccgtggcc ccatgatcat
     1381 gagcggctac gttaacaacc ccgaggctac aaacgctctc atcgacaagg acggctggct
     1441 gcacagcggc gacatcgcct actgggacga ggacgagcac ttcttcatcg tggaccggct
     1501 gaagagcctg atcaaataca agggctacca ggtagcccca gccgaactgg agagcatcct
     1561 gctgcaacac cccaacatct tcgacgccgg ggtcgccggc ctgcccgacg acgatgccgg
     1621 cgagctgccc gccgcagtcg tcgtgctgga acacggtaaa accatgaccg agaaggagat
     1681 cgtggactat gtggccagcc aggttacaac cgccaagaag ctgcgcggtg gtgttgtgtt
     1741 cgtggacgag gtgcctaaag gactgaccgg caagttggac gcccgcaaga tccgcgagat
     1801 tctcattaag gccaagaagg gcggcaagat cgccgtgtaa taattctaga gtcggggcgg
     1861 ccggccgctt cgagcagaca tgataagata cattgatgag tttggacaaa ccacaactag
     1921 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
     1981 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt
     2041 tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtaaaat
     2101 cgataaggat ccagggataa ctctttactg ccacaccctt tgaaaatgga gatgtttacc
     2161 ccctctgtcc ctagtagccc agacgagcct caaacttggc tgtatagctg agcatgacct
     2221 tgaactgttg ctccttctgt cccggattcc caagggctgg cattaccggc tggtatcacc
     2281 tgatttacga ggttgctttc ttttgctggt ggtattcaac tgcaaagttg agctatcaag
     2341 tcattggcaa agaggacaaa gcctttggat ttggaggtgg cccacgaatt ccacagatcc
     2401 gggaagcaca ggcccacgtc ctccactcca aaggatcatt aacagatcct gactgtagtg
     2461 gtgctgacgg cagggagtat agaacccaca acgtgaccgg aacaagcctg tggtgattgc
     2521 ccggaaagca gccaggccac ctgcagcatc cctgcttcaa ggtcaactga aaagcctggg
     2581 ctccagcaga gtcgaccgat gcccttgaga gccttcaacc cagtcagctc cttccggtgg
     2641 gcgcggggca tgactatcgt cgccgcactt atgactgtct tctttatcat gcaactcgta
     2701 ggacaggtgc cggcagcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     2761 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     2821 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     2881 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     2941 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     3001 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     3061 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     3121 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     3181 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     3241 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     3301 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     3361 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     3421 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     3481 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     3541 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     3601 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     3661 cggccgcaaa tgctaaacca ctgcagtggt taccagtgct tgatcagtga ggcaccgatc
     3721 tcagcgatct gcctatttcg ttcgtccata gtggcctgac tccccgtcgt gtagatcact
     3781 acgattcgtg agggcttacc atcaggcccc agcgcagcaa tgatgccgcg agagccgcgt
     3841 tcaccggccc ccgatttgtc agcaatgaac cagccagcag ggagggccga gcgaagaagt
     3901 ggtcctgcta ctttgtccgc ctccatccag tctatgagct gctgtcgtga tgctagagta
     3961 agaagttcgc cagtgagtag tttccgaaga gttgtggcca ttgctactgg catcgtggta
     4021 tcacgctcgt cgttcggtat ggcttcgttc aactctggtt cccagcggtc aagccgggtc
     4081 acatgatcac ccatattatg aagaaatgca gtcagctcct tagggcctcc gatcgttgtc
     4141 agaagtaagt tggccgcggt gttgtcgctc atggtaatgg cagcactaca caattctctt
     4201 accgtcatgc catccgtaag atgcttttcc gtgaccggcg agtactcaac caagtcgttt
     4261 tgtgagtagt gtatacggcg accaagctgc tcttgcccgg cgtctatacg ggacaacacc
     4321 gcgccacata gcagtacttt gaaagtgctc atcatcggga atcgttcttc ggggcggaaa
     4381 gactcaagga tcttgccgct attgagatcc agttcgatat agcccactct tgcacccagt
     4441 tgatcttcag catcttttac tttcaccagc gtttcggggt gtgcaaaaac aggcaagcaa
     4501 aatgccgcaa agaagggaat gagtgcgaca cgaaaatgtt ggatgctcat actcgtcctt
     4561 tttcaatatt attgaagcat ttatcagggt tactagtacg tctctcaagg ataagtaagt
     4621 aatattaagg tacgggaggt attggacagg ccgcaataaa atatctttat tttcattaca
     4681 tctgtgtgtt ggttttttgt gtgaatcgat agtactaaca tacgctctcc atcaaaacaa
     4741 aacgaaacaa aacaaactag caaaataggc tgtccccagt gcaagtgcag gtgccagaac
     4801 atttctct
//
