LOCUS       sequence-23657-e         818 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Arking DE, Krebsova A, Macek M, Macek M, Arking A, Mian IS, Fried L,
            Hamosh A, Dey S, McIntosh I, Dietz HC
  TITLE     Association of human aging with a functional variant of klotho.
  JOURNAL   Proc Natl Acad Sci U S A. 2002 Jan 22. 99(2):856-61.
  PUBMED    11792841
REFERENCE   2  (bases 1 to 818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2002 Jan 22. 99(2):856-61."
COMMENT     Sequence Label: Klotho (secreted)
FEATURES             Location/Qualifiers
     source          1..818
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(7..24)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(34..75)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 gttatcatgg tgatggtgat gatgaccggt acgcgtagaa tcgagaccga ggagagggtt
       61 agggataggc ttaccttcga accgcgggcc ctctagactc gagcggccgc cactgtgctg
      121 gatatctgca gaattgccct tgtgataggg cttggtgaga ctgctgattg gttttgtcag
      181 ctgacttact tgaatgtagt tgtcaacaac tccccaagca aagtcacagg gaaatgtccc
      241 ttctaggggc tgattttcag gtaagggagg gaagccattt ttctctatca gcttttggta
      301 gaacaaggct gaagactttg gcaacaacat cttgtcctgg cttagaaagt caacatagaa
      361 gagtccacgc ctgatgctgt aacctctgtg ccactcgaaa ccatccatga gggaccatgc
      421 ggtatacccg atgacatcca ccccatccag cttgatggct tttaaggttt ccatgatgaa
      481 ctttttgagg taatacatat atttggcatc atctctcttg gtggtccctg agacaaacca
      541 gccattttcc acaataaata tttgaggatg gttaaattca aggtcaatcc aggaaagcag
      601 ttgcctcagg ttgnanancc nnnttgggcg gaacttcatn ncaagggtcc aaaagttgaa
      661 aactcaaggt gggtccnaag caaagagcaa aaaagtcagc agttcctttg atgaattttt
      721 tcncaatnct cnaaaaacgg gngaaaatag aaanggtttt cnntccgcnc cggnaagtca
      781 catcataaat acgggttggc aacnacctag tacaagtc
//
