LOCUS       sequence-23671-e        1011 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Nallamsetty S, Kapust RB, Tozser J, Cherry S, Tropea JE, Copeland 
            TD, Waugh DS
  TITLE     Efficient site-specific processing of fusion proteins by tobacco 
            vein mottling virus protease in vivo and in vitro.
  JOURNAL   Protein Expr Purif. 2004 Nov . 38(1):108-15.
  PUBMED    15477088
REFERENCE   2  (bases 1 to 1011)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif. 2004 Nov . 38(1):108-15."
COMMENT     Sequence Label: pRK1036
FEATURES             Location/Qualifiers
     source          1..1011
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             24..44
                     /codon_start=1
                     /product="tobacco vein mottling virus (TVMV) NIa protease 
                     recognition and cleavage site
                     "
                     /label=TVMV site
                     /translation="ETVRFQS"
     CDS             789..806
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    complement(812..836)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(848..865)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(848..864)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(857..879)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gacagttgtc aaaagcaggc tcggagaccg tgcgtttcca gagcatgagc gagcaacagg
       61 ttcaggaact tgagaaaaag tggtacgcac tgcaggtaga gcccggaaaa gagaacgaag
      121 ctaaggaaaa cttactgaaa gttctggaac tggaaggttt aaaggacctc gttgacgagg
      181 taatagttcc cgcagaggaa aaggtagtca taagggctca gggcaaggaa aagtacaggc
      241 tctctctcaa gggaaacgcg agggacatat ccgttttagg gaagaaaggt gttaccacat
      301 tcaggataga gaacggagaa gttaaggtcg ttgagagtgt tgaaggagac acttgcgtaa
      361 acgcccctcc catatcaaaa cccggacaaa agatcacgtg taaggaaaat aaaacggaag
      421 caaagatagt tctggataac aaaatatttc ccggatacat actcataaag gctcacatga
      481 acgacaagct cctcatggct attgagaaaa ctccccacgt cttcagacct gtaatggtgg
      541 gcggaaagcc cgttcctctg aaagaggaag aggttcaaaa cattctcaat caaataaaga
      601 ggggtgttaa accttccaag gttgagtttg aaaaaggaga tcaggtaagg gttatagaag
      661 gaccgtttat gaactttacg ggaactgttg aggaagttca tcccgagaag aggaaattaa
      721 ctgttatgat aagtattttc ggaaggatga ctccggtaga actggacttt gaccaagtgg
      781 aaaagattca tcaccaccat catcactaat aacccagctt tcttgtacaa agtggtgata
      841 gcttggcact ggccgtcgtt ttacaacgtc gtgactggga aanccctggc gttacccaac
      901 ttaatcgcct ngcaccacat nccccctttc gccagcttgg cgtaatagcg angaggcccg
      961 cacccgatcg ccccttccca acagtttgcg ccagcctgaa tggcgaatgg c
//
