LOCUS       sequence-23681-e         882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Dai C, Whitesell L, Rogers AB, Lindquist S
  TITLE     Heat shock factor 1 is a powerful multifaceted modifier of 
            carcinogenesis.
  JOURNAL   Cell. 2007 Sep 21. 130(6):1005-18.
  PUBMED    17889646
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2007 
            Sep 21. 130(6):1005-18."
COMMENT     Sequence Label: pBabe-cmv-hrGFP-zeo
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             76..795
                     /codon_start=1
                     /product="humanized Renilla green fluorescent protein"
                     /label=hrGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKQILKNTGLQEIMSFKVNLEGVVNNHVFTMEGCGKGNILFGN
                     QLVQIRVTKGAPLPFAFDILSPAFQYGNRTFTKYPEDISDFFIQSFPAGFVYERTLRYE
                     DGGLVEIRSDINLIEEMFVYRVEYKGRNFPNDGPVMKKTITGLQPSFEVVYMNDGVLVG
                     QVILVYRLNSGKFYSCHMRTLMKSKGVVKDFPEYHFIQHRLEKTYVEDGGFVEQHETAI
                     AQLTSLGKPLGSLHEWV"
     primer_bind     complement(832..852)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 cgtgggcntt agcgagctgg ttngtgaccg tcagatccgc tagcgctacc ggactcagat
       61 ctcgacctcg agaccatggt gagcaagcag atcctgaaga acaccggcct gcaggagatc
      121 atgagcttca aggtgaacct ggagggcgtg gtgaacaacc acgtgttcac catggagggc
      181 tgcggcaagg gcaacatcct gttcggcaac cagctggtgc agatccgcgt gaccaagggc
      241 gcccccctgc ccttcgcctt cgacatcctg agccccgcct tccagtacgg caaccgcacc
      301 ttcaccaagt accccgagga catcagcgac ttcttcatcc agagcttccc cgccggcttc
      361 gtgtacgagc gcaccctgcg ctacgaggac ggcggcctgg tggagatccg cagcgacatc
      421 aacctgatcg aggagatgtt cgtgtaccgc gtggagtaca agggccgcaa cttccccaac
      481 gacggccccg tgatgaagaa gaccatcacc ggcctgcagc ccagcttcga ggtggtgtac
      541 atgaacgacg gcgtgctggt gggccaggtg atcctggtgt accgcctgaa cagcggcaag
      601 ttctacagct gccacatgcg caccctgatg aagagcaagg gcgtggtgaa ggacttcccc
      661 gagtaccact tcatccagca ccgcctggag aagacctacg tggaggacgg cggcttcgtg
      721 gagcagcacg agaccgccat cgcccagctg accagcctgg gcaagcccct gggcagcctg
      781 cacgagtggg tgtaataggt aggaattcgc cagcacagtg gtcgaccctg tggaatgtgt
      841 gtcagttagg gtgtggaanc ccagntccca cagcaaattg aa
//
