LOCUS       sequence-23688-e         977 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Ruby JG, Jan CH, Bartel DP
  TITLE     Intronic microRNA precursors that bypass Drosha processing.
  JOURNAL   Nature. 2007 Jul 5. 448(7149):83-6.
  PUBMED    17589500
REFERENCE   2  (bases 1 to 977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2007 Jul 5. 448(7149):83-6."
COMMENT     Sequence Label: CJ19 (miR-1006 minigene)
FEATURES             Location/Qualifiers
     source          1..977
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             848..865
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(888..905)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     primer_bind     complement(931..950)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tctgtacacg aatacatcca gtcggtggcc cggtgcctgc agatgatgct ccgtgtggat
       61 gagtatcgat ttgcctttgt gggagtcgac ggaatcagca ctctgatccg tatcttgtcg
      121 acccgtgtca acttccaggt gagtttgaaa ttgaaatgcg taaattgttt ggtacaattt
      181 aaattcgatt tcttattcat aggtgcaata ccagttgatc ttttgcctgt gggtgctgac
      241 cttcaatccc ctgctggccg ccaagatgaa taagttcagc gtgatcccca tcctggctga
      301 tatcctcagc gattgtgcca aggagaaggt gacacgcatt atcctcgcgg tcttccgcaa
      361 tctgatcgag aagccggagg attcatcggt ggccaaggac cattgcatcg ccatggtcca
      421 gtgcaaggtg ctgaagcagc tatccatcct ggagcagcgt cgcttcgacg acgaggacat
      481 taccgccgac gtagagtacc tgagcgagaa gctccagaat tcggtgcaag acttgagctc
      541 ctttgatgag tacgccacag aggtgcgcag cggtcgcttg gaatggtcgc ctgtgcacaa
      601 gtcggccaag ttctggcgcg agaatgccca gcgcctaaac gaaaagaact acgagttgct
      661 gcgcatcctc gtccacctcc tggaaacctc aaaagatgcc atcatccttt ccgtcgcctg
      721 cttcgacatc ggggagtatg tgcgccacta tccccgcggc aagcagcggc cgctcgagtc
      781 tagagggccc ttcgaangta agcctatccc taaccctctc ctcggtctcg attctacgcg
      841 taccggtcat catcaccatc accattgagt ttaaacccgc tgatcagcct cgactgtgcc
      901 ttctaagatc cagacatgat aagatacatt gatgagtttg gacaaaccac aactaatgat
      961 gaaaagttat ggattgg
//
