LOCUS       sequence-23690-e         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Ruby JG, Jan CH, Bartel DP
  TITLE     Intronic microRNA precursors that bypass Drosha processing.
  JOURNAL   Nature. 2007 Jul 5. 448(7149):83-6.
  PUBMED    17589500
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2007 Jul 5. 448(7149):83-6."
COMMENT     Sequence Label: CJ22 (miR-1003 perfect site)
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             190..231
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             241..258
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(281..298)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    300..434
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(324..343)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     378..397
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(639..659)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 ctgaatttgt caagtaaaag gtcttcattt ttcgcaagaa gatgcacctg atgaaatggg
       61 aaaatatatc aaatcgttcg ttgagcgagt tctcaaaaat gaacaataat tctaggagct
      121 cctgtgaata tgtaaatgtg agaactagtt gttctagagc ggccgctcga gtctagaggg
      181 cccttcgaag gtaagcctat ccctaaccct ctcctcggtc tcgattctac gcgtaccggt
      241 catcatcacc atcaccattg agtttaaacc cgctgatcag cctcgactgt gccttctaag
      301 atccagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
      361 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
      421 gcaataaaca agttctagag tcggtgggcc tcgggggcgg gtgcggggtc ggcggggccg
      481 ccccgggtgg cttcggtcgg agccatgggg tcgtgcgctc ctttcggtcg ggcgctgcgg
      541 gtcgtggggc gggcgtcagg caccgggctt gcgggtcatg caccaggtgc gcggtccttc
      601 gggcacctcg acgtcggcgg tgacggtgaa gccgagccgc tcgtagaagg ggaggttgcg
      661 gggcgcggag gtctccagga aggcgggcac cccggcgcgc tcggccgcct ccactccggg
      721 gagcacgacg gcgctgccca gacccttgcc ctggtggtcg ggcgagacgc cgacggtggc
      781 caggaaccac gcgggctcct tgggccggtg cggcgccagg aggccttcca tctgtgctgc
      841 gcggccagcn ggaccgctca ctcggcatgc gcggccgatt cgngaaacga
//
