LOCUS       sequence-236903-       14639 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Targets the human CLYBL safe harbor locus for integration of 
            dox-inducible NGN2-ISL1-LHX3 for differentiation of iPSCs into lower
            motor neurons. Contains a floxed BSD-NLS-mApple selection cassette..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14639)
  TITLE     Next generation vectors for transcription factor mediated 
            differentiation of iPSCs into cortical and motor neurons
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14639)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: CLYBL-TO-hNIL-BSD-mApple
FEATURES             Location/Qualifiers
     source          1..14639
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     266..285
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2052..2429
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     protein_bind    2059..2077
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2095..2113
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2131..2149
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2167..2185
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2203..2221
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2239..2257
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2275..2293
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2338..2362
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3273..3329
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     regulatory      4120..4129
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4401..4454
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     polyA_signal    5700..5821
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5737..5756)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5791..5810
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5870..5886)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5870..5886)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5883..5905)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5894..5910
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5918..5924,5925..5942,5943..5948))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5963..5984
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6101..6118)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     6410..6429
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    6428..6483
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     6529..6548
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(6639..7385)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(7417..7436)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          7444..8461
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(7498..7520)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     promoter        complement(8463..8740)
                     /label=chicken beta-actin promoter
     enhancer        8742..9121
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     polyA_signal    9127..9351
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     9340..9357
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     protein_bind    9373..9406
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      10184..10193
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(10242..10268)
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             complement(10284..10310)
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             complement(10317..10370)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     LTR             10839..11107
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     promoter        complement(11120..11331)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     polyA_signal    11367..11591
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(11596..12396)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="GPRIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQG
                     RPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEE
                     HQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQD
                     IALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(11707..11726)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     12317..12336
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     CDS             complement(12391..12444)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    12468..12493
                     /label=SA
                     /note="splice acceptor site"
     protein_bind    12494..12527
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             join(13385..13453,13454..14245)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(13603..13622)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(14416..14639,1..365)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
       61 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
      121 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
      181 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
      241 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
      301 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
      361 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc gaattcgccc ttagagcaga
      421 gccaggaacc cctgtaggga aggggcagga gagccagggg catgagatgg tggacgagga
      481 agggggacag ggaagcctga gcgcctctcc tgggcttgcc aaggactcaa acccagaagc
      541 ccagagcagg gccttaggga agcgggacgg ctctgttgga gagtccagta ttgaattagt
      601 gatagataca cagaaaaaaa gcaaattgaa taggtgaatg gataaacaaa ctgtggaaca
      661 tccagacaat gaaatattca ccaataaaaa gaaatgagct atcaagccac attggctatg
      721 cccttcacat ttaaggagga accttaaatg catattgcca aattttaaaa gctagtctga
      781 aaatgctaca gactgtatga ttccaattat atgacatttt agaaaaggca aaactataga
      841 gatcagtggt tgccagcggc tcatggaggc aggatgaatt ggtggaacac agggatgctt
      901 tcagggtaat gaaaatatct tgtgtgatcc tgtaataatg gatacgtggc ataatgcatt
      961 tgttaaaacc tgtaaactag ccaggtatag tggctttttt gcacctatag tctcagctac
     1021 ttggtaggct gagacaggaa gatcacttga gcccaggact tcaagaccag cctgggtaat
     1081 atagtaagac cccatctcta ttttttaaag aaattaagaa agaaaagaaa cccatcaact
     1141 gtataacaca aagaatcgta atgtaaacta cagtcctgag taatgaaaca tttttaaaga
     1201 aataaataaa ttcaaggcaa ttattaactt caggaaaaac agtaaagtaa acataagtat
     1261 acaaatcaaa catggctcag ttgtgaaaat atttacaagg tcactttaag taacattgaa
     1321 tgttgatata atcccaaaat atatttatgt tggaaggatg aggagattta attaagggag
     1381 gtggtccctg cagttacgcc aatgataacc cccgccagaa aaatcttagt agccttccct
     1441 ttttgttttc cgtgccccaa ctcggcggat tgactcggcc ccttccggaa acacccgaat
     1501 caacttctag tcaaattatt gttcacgccg caatgaccca cccctggccc gcgtctgtgg
     1561 aactgacccc tggtgtacag gagagttcgc tgctgaaagt ggtcccaaag gggtactagt
     1621 ttttaagctc ccaactcccc ctcccccagc gtctggagga ttccacaccc tcgcaccgca
     1681 ggggcgagga agtgggcgga gtccggtttt ggcgccagcc gctgaggctg ccaagcagaa
     1741 aagccaccgc tgaggagact ccggtcactg tcctcgcccc gcctccccct tccctcccct
     1801 tggggaccac cgggcgccac gccgcgaacg gtaagtgccg cggtcgtcgg cgcctccgcc
     1861 ctccccctag ggccccaatt cccagcgggc gcggcgcgcg gcccctcccc ccgccgggcg
     1921 cgcgcccgct gccccgccct tcgtggccgc ccggcgtggg cggtgccacc cctccccccg
     1981 gcggccccgc gcgcagctcc cggctccctc ccccttcgga tgtggcttga gctgtaggcg
     2041 cggagggtac cagtttactc cctatcagtg atagagaacg tatgaagagt ttactcccta
     2101 tcagtgatag agaacgtatg cagactttac tccctatcag tgatagagaa cgtataagga
     2161 gtttactccc tatcagtgat agagaacgta tgaccagttt actccctatc agtgatagag
     2221 aacgtatcta cagtttactc cctatcagtg atagagaacg tatatccagt ttactcccta
     2281 tcagtgatag agaacgtata agctttaggc gtgtacggtg ggcgcctata aaagcagagc
     2341 tcgtttagtg aaccgtcaga tcgcctggag caattccaca acacttttgt cttataccaa
     2401 ctttccgtac cacttcctac cctcgtaaag gatccgccgc caccatgttc gtcaaatccg
     2461 agaccttgga gttgaaggag gaagaggacg tgttagtgct gctcggatcg gcctcccccg
     2521 ccttggcggc cctgaccccg ctgtcatcca gcgccgacga agaagaggag gaggagccgg
     2581 gcgcgtcagg cggggcgcgt cggcagcgcg gggctgaggc cgggcagggg gcgcggggcg
     2641 gcgtggctgc gggtgcggag ggctgccggc ccgcacggct gctgggtctg gtacacgatt
     2701 gcaaacggcg cccttcccgg gcgcgggccg tctcccgagg cgccaagacg gccgagacgg
     2761 tgcagcgcat caagaagacc cgtagactga aggccaacaa ccgcgagcga aaccgcatgc
     2821 acaacctcaa cgcggcactg gacgcgctgc gcgaggtgct ccccacgttc cccgaggacg
     2881 ccaagctcac caagatcgag accctgcgct tcgcccacaa ctacatctgg gcactcaccg
     2941 agaccctgcg cctggcggat cactgcgggg gcggcggcgg gggcctgccg ggggcgctct
     3001 tctccgaggc agtgttgctg agcccgggag gcgccagcgc cgccctgagc agcagcggag
     3061 acagcccctc gcccgcctcc acgtggagtt gcaccaacag ccccgcgccg tcctcctccg
     3121 tgtcctccaa ttccacctcc ccctacagct gcactttatc gcccgccagc ccggccgggt
     3181 cagacatgga ctattggcag cccccacctc ccgacaagca ccgctatgca cctcacctcc
     3241 ccatagccag ggattgtatc ttgggatccg gagctactaa cttcagcctg ctgaagcagg
     3301 ctggagacgt ggaggagaac cctggaccta tgggagacat gggagatcca ccaaaaaaaa
     3361 aacgtctgat ttccctatgt gttggttgcg gcaatcagat tcacgatcag tatattctga
     3421 gggtttctcc ggatttggaa tggcatgcgg catgtttgaa atgtgcggag tgtaatcagt
     3481 atttggacga gagctgtaca tgctttgtta gggatgggaa aacctactgt aaaagagatt
     3541 atatcaggtt gtacgggatc aaatgcgcca agtgcagcat cggcttcagc aagaacgact
     3601 tcgtgatgcg tgcccgctcc aaggtgtatc acatcgagtg tttccgctgt gtggcctgca
     3661 gccgccagct catccctggg gacgaatttg cgcttcggga ggacggtctc ttctgccgag
     3721 cagaccacga tgtggtggag agggccagtc taggcgctgg cgacccgctc agtcccctgc
     3781 atccagcgcg gccactgcaa atggcagcgg agcccatctc cgccaggcag ccagccctgc
     3841 ggccccacgt ccacaagcag ccggagaaga ccacccgcgt gcggactgtg ctgaacgaga
     3901 agcagctgca caccttgcgg acctgctacg ccgcaaaccc gcggccagat gcgctcatga
     3961 aggagcaact ggtagagatg acgggcctca gtccccgtgt gatccgggtc tggtttcaaa
     4021 acaagcggtg caaggacaag aagcgaagca tcatgatgaa gcaactccag cagcagcagc
     4081 ccaatgacaa aactaatatc caggggatga caggaactcc catggtggct gccagtccag
     4141 agagacacga cggtggctta caggctaacc cagtggaagt acaaagttac cagccacctt
     4201 ggaaagtact gagcgacttc gccttgcaga gtgacataga tcagcctgct tttcagcaac
     4261 tggtcaattt ttcagaagga ggaccgggct ctaattccac tggcagtgaa gtagcatcaa
     4321 tgtcctctca acttccagat acacctaaca gcatggtagc cagtcctatt gaggcattga
     4381 tgcacagcag aggctctggc gagggcagag gcagcctgct gacatgtggc gacgtggaag
     4441 agaaccctgg ccccggtacc atggaagcca gaggcgaact gggccctgcc agagaatctg
     4501 ccggcggaga tctgctgctg gccctgctgg ctagaagggc cgacctgaga agagaaatcc
     4561 ccctgtgcgc cggctgcgac cagcacatcc tggaccggtt tatcctgaag gccctggaca
     4621 gacactggca cagcaagtgc ctgaagtgca gcgactgcca cacccccctg gccgagagat
     4681 gctttagcag aggcgagagc gtgtactgca aggacgactt cttcaagaga ttcggcacca
     4741 agtgcgccgc ctgccagctg ggaattcctc ctacacaggt cgtgcggaga gcccaggact
     4801 tcgtgtacca tctgcactgc ttcgcctgcg tcgtgtgcaa gagacagctg gccaccggcg
     4861 acgagttcta cctgatggaa gatagccggc tcgtgtgtaa agccgactac gagacagcca
     4921 agcagagaga ggccgaggcc accgccaaaa gacccagaac caccatcacc gccaagcagc
     4981 tggaaaccct gaagtccgcc tacaacacca gccccaagcc cgccagacat gtgcgggaac
     5041 agctgagcag cgagacaggc ctggacatga gagtggtgca agtgtggttc cagaacagac
     5101 gggccaaaga gaagcggctg aagaaggacg ccggcaggca gagatggggc cagtacttcc
     5161 ggaacatgaa gcggtccaga ggcggcagca agagcgacaa ggacagcgtg caggaaggcc
     5221 aggacagcga cgccgaggtg tccttcccag atgagcctag cctggccgaa atgggcccag
     5281 ccaatggcct gtatggcagc ctgggagagc caacccaggc tctgggcaga ccttctggcg
     5341 ccctgggcaa cttctctctg gaacacggcg gactggccgg acccgagcag tatagagaac
     5401 tgaggcccgg cagcccttac ggcgtgccac cttctccagc tgcccctcag tctctgccag
     5461 gacctcagcc tctgctgtcc agcctggtgt accccgatac aagcctgggc ctggtgccat
     5521 ctggcgctcc aggcggacct cctccaatga gagtgctggc cggcaatggc cctagcagcg
     5581 atctgagcac aggcagctct ggcggctacc ccgattttcc tgccagccct gcctcctggc
     5641 tggacgaagt ggatcacgcc cagttctgag tttaaactcg cgaatcgatc gcccacccca
     5701 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     5761 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     5821 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
     5881 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     5941 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     6001 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     6061 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     6121 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggacggc cgcacatgta
     6181 aggaaaattt tagggatgtt aaagaaaaaa ataacacaaa acaaaatata aaaaaaatct
     6241 aacctcaagt caaggctttt ctatggaata aggaatggac agcagggggc tgtttcatat
     6301 actgatgacc tctttatagc caacctttgt tcatggcagc cagcatatgg gcatatgttg
     6361 ccaaactcta aaccaaatac tcattctgat gttttaaatg atttgccctc ccatatgtcc
     6421 ttccgagtga gagacacaaa aaattccaac acactattgc aatgaaaata aatttccttt
     6481 attagccaga agtcagatgc tcaaggggct tcatgatgtc cccataattt ttggcagagg
     6541 gaaaaagatc tcagtggtat ttgtgagcca gggcattggc cacaccagcc accaccttct
     6601 gataggcagc ctgcacctga ggagtgaatt cacgcgtttt agcctggcag catatcgaga
     6661 tcgaagtcat cgagagcgtc agcagggagc atatccagat caaaatcatc cagggcatcg
     6721 gcgggcagca tgtccaggtc gaagtcgtcc agggcgtcgg tgggtccgcc gctctcgcac
     6781 ttcagctgct tctcgaggcc gcagatgatc agttccaggc cgaacaggaa ggcgggctcg
     6841 gcgccctgtc tgtcgaacag ctcgatggcc tgcttcagca gggggggcat gctgtcggtg
     6901 gtgggtgtct ctctttcctc tttggcgacc tggtgttcct gttcttccag cacgcagccc
     6961 agggtgaagt ggcccacggc gctcagggcg tacagggcgt tttccaggct gaagccctgc
     7021 tggcacagga aggccagctg gttttccagt gtctcgtact gcttctcggt gggtctggtg
     7081 cccaggtgca ctttggcgcc gtcccggtgg ctcagcaggg cgcatctgta gctcttggcg
     7141 ttgttccgca ggaagtcctg ccagctctcg ccttccagag ggcagctgtg ggtgtggtgc
     7201 cggtccagca tctcgatggg cagggcgtcc agcagggccc gcttgttctt cacgtgccag
     7261 tacagggtgg gctgttccac gcccagtttc tgggccagct tccgggtggt caggccctcg
     7321 atgcccacgc cgttcagcag ttccagggcg ctgttgatca ctttgctctt gtccagccgg
     7381 ctcatgtcga cattctttgc caaaatgatg agacagcaca ataaccagca cgttgcccag
     7441 gagctgtagg aaaaagaaga aggcatgaac atggttagca gaggctctag agccgccggt
     7501 cacacgccag aagccgaacc ccgccctgcc ccgtcccccc cgaaggcagc cgtccccccg
     7561 cggacagccc cgaggctgga gagggagaag gggacggcgg cgcggcgacg cacgaaggcc
     7621 ctccccgccc atttccttcc tgccggcgcc gcaccgcttc gccccgcgcc cgctagaggg
     7681 ggtgcggcgg cgcctcccag atttcggctc cgcacagatt tgggacaaag gaagtccctg
     7741 cgccctctcg cacgattacc ataaaaggca atggctgcgg ctcgccgcgc ctcgacagcc
     7801 gccggcgctc cgggggccgc cgcgcccctc ccccgagccc tccccggccc gaggcggccc
     7861 cgccccgccc ggcaccccca cctgccgcca ccccccgccc ggcacggcga gccccgcgcc
     7921 acgccccgta cggagccccg cacccgaagc cgggccgtgc tcagcaactc ggggaggggg
     7981 gtgcaggggg ggttgcagcc cgaccgacgc gcccacaccc cctgctcacc cccccacgca
     8041 cacaccccgc acgcagcctt tgttcccctc gcagcccccc ccgcaccgcg gggcaccgcc
     8101 cccggccgcg ctcccctcgc gcacactgcg gagcgcacaa agccccgcgc cgcgcccgca
     8161 gcgctcacag ccgccgggca gcgcggagcc gcacgcggcg ctccccacgc acacacacac
     8221 gcacgcaccc cccgagccgc tccccccgca caaagggccc tcccggagcc cctcaaggct
     8281 ttcacgcagc cacagaaaag aaacaagccg tcattaaacc aagcgctaat tacagcccgg
     8341 aggagaaggg ccgtcccgcc cgctcacctg tgggagtaac gcggtcagtc agagccgggg
     8401 cgggcggcgc gaggcggcgg cggagcgggg cacggggcga aggcagcgtc gcagcgactc
     8461 cccgcccgcc gcgcgcttcg ctttttatag ggccgccgcc gccgccgcct cgccataaaa
     8521 ggaaactttc ggagcgcgcc gctctgattg gctgccgccg cacctctccg cctcgccccg
     8581 ccccgcccct cgccccgccc cgccccgcct ggcgcgcgcc cccccccccc ccccgccccc
     8641 atcgctgcac aaaataatta aaaaataaat aaatacaaaa ttgggggtgg ggaggggggg
     8701 gagatgggga gagtgaagca gaacgtgggg ctcacctcga ccatggtaat agcgatgact
     8761 aatacgtaga tgtactgcca agtaggaaag tcccataagg tcatgtactg ggcataatgc
     8821 caggcgggcc atttaccgtc attgacgtca atagggggcg tacttggcat atgatacact
     8881 tgatgtactg ccaagtgggc agtttaccgt aaatactcca cccattgacg tcaatggaaa
     8941 gtccctattg gcgttactat gggaacatac gtcattattg acgtcaatgg gcgggggtcg
     9001 ttgggcggtc agccaggcgg gccatttacc gtaagttatg taacgcggaa ctccatatat
     9061 gggctatgaa ctaatgaccc cgtaattgat tactattaat aactagtcaa taatcaatgt
     9121 cgagacccat agagcccacc gcatccccag catgcctgct attgtcttcc caatcctccc
     9181 ccttgctgtc ctgccccacc ccacccccca gaatagaatg acacctactc agacaatgcg
     9241 atgcaatttc ctcattttat taggaaagga cagtgggagt ggcaccttcc agggtcaagg
     9301 aaggcacggg ggaggggcaa acaacagatg gctggcaact agaaggcaca gtcgaggctg
     9361 atcagcgggt ttataacttc gtataatgta tgctatacga agttatggta ccttactcgt
     9421 ccatgccgcc ggtggagtgg cggccctcgg cgcgttcgta ctgttccacg atggtgttac
     9481 ttgtacagct cgtccattcc gcctgtgctg tgtctgcctt cggctctctc gtactgttcc
     9541 acgatggtgt agtcctcgtt gtgggacacg atgtccagct tgatatccac gatgtaggcg
     9601 ccaggcagct gcacgggttt cttggccttg taggtggttt tcacttcggc ggcatagtgg
     9661 ccgccatcct tcagcttcag ccgcttcttg atctcggact tcagggcgcc atcctcaggg
     9721 tacattcttt cctcgctggc ttcccagccc atggtctttt tctgcatcac ggggccatca
     9781 gagggaaagt tggtgccccg cagcttcact ttgtagatga acacgccgtc ctgcagagag
     9841 ctatcctggt tcacgtggat gatgccgccg tcctcgaagt tcatcactct ctcccatctg
     9901 aagccctcgg ggaagctcag cttgaagtag tcgggaatgt cggcggggtg cttgatgtac
     9961 accttgctgc cgtacataaa ctgagggctc agaatgtccc aggcaaaagg cagagggccg
    10021 cctttggtca ctttcagctt ggcggtctga aaagcctcgt agggtctgcc ctcgccttcg
    10081 ccttcaatct cgaactcgtg gccgttcacg ctgccttcca tgtgcacctt gaaccgcatg
    10141 aattctttga tgatggccat gttgttctct tcgcccttgg acaccatggt ggcgaccggt
    10201 agcgtggcga ccggggtggc ggtggctccc cctgaccctc catccaactt aacgcgcttt
    10261 gcagcaggac ctccagaacc gccgtcgagt ttgacccgtt tagcggcagg gaccggtggg
    10321 ccaggattct cctcgacgtc accgcatgtt agcagacttc ctctgccctc tcccgatccc
    10381 gatccatttc gggtatattt gagtggaatg agttcttcaa tcgtagtttt gactaacttg
    10441 ccattcattt ctattaacac aaaacaatct ggtgcatagt ctgaaatcaa ctccctacac
    10501 ataccacaag gacttaccac tcgaatactt ctatctactt cgtcagaata agggtgtcta
    10561 acagctacaa tcgtgtcaaa atccttttgt ccattcgaaa ctgcactacc aatcgcaatg
    10621 gcttctgcac aaacagttac tcgtcctata tacgcttcaa tatgtactgc cgaaatgatt
    10681 tctcctgttt tcgtacgaat tgccgctccc acatgatgtt tattatcctc ataaagcatt
    10741 gtaatcttct ctgtcgctac ttctactaat tctagatcct gctgggagat gttgaaggtc
    10801 ttcatggtgg cgctagcgta ggcgccggtc acagcttgga tctgtaacgg cgcagaacag
    10861 aaaacgaaac aaagacgtag agttgagcaa gcagggtcag gcaaagcgtg gagagccggc
    10921 tgagtctagg taggctccaa gggagcgccg gacaaaggcc cggtctcgac ctgagcttta
    10981 aacttaccta gacggcggac gcagttcagg aggcaccaca ggcgggaggc ggcagaacgc
    11041 gactcaaccg gcgtggatgg cggcctcagg tagggcggcg ggcgcgtgaa ggagagatgc
    11101 gagcccctcg aagcttcagc tgtgttctgg cggcaaaccc gttgcgaaaa agaacgttca
    11161 cggcgactac tgcacttata tacggttctc ccccaccctc gggaaaaagg cggagccagt
    11221 acacgacatc actttcccag tttaccccgc gccaccttct ctaggcaccg gttcaattgc
    11281 cgacccctcc ccccaacttc tcggggactg tgggcgatgt gcgctctgcc cactgacggg
    11341 caccggagcg atcgcagatc atgcatccat agagcccacc gcatccccag catgcctgct
    11401 attgtcttcc caatcctccc ccttgctgtc ctgccccacc ccacccccca gaatagaatg
    11461 acacctactc agacaatgcg atgcaatttc ctcattttat taggaaagga cagtgggagt
    11521 ggcaccttcc agggtcaagg aaggcacggg ggaggggcaa acaacagatg gctggcaact
    11581 agaaggcaca ggtttttaga agaactcgtc aagaaggcga tagaaggcga tgcgctgcga
    11641 atcgggagcg gcgataccgt aaagcacgag gaagcggtca gcccattcgc cgccaagctc
    11701 ttcagcaata tcacgggtag ccaacgctat gtcctgatag cggtccgcca cacccagccg
    11761 gccacagtcg atgaatccag aaaagcggcc attttccacc atgatattcg gcaagcaggc
    11821 atcgccatgg gtcacgacga gatcctcgcc gtcgggcatg cgcgccttga gcctggcgaa
    11881 cagttcggct ggcgcgagcc cctgatgctc ttcgtccaga tcatcctgat cgacaagacc
    11941 ggcttccatc cgagtacgtg ctcgctcgat gcgatgtttc gcttggtggt cgaatgggca
    12001 ggtagccgga tcaagcgtat gcagccgccg cattgcatca gccatgatgg atactttctc
    12061 ggcaggagca aggtgagatg acaggagatc ctgccccggc acttcgccca atagcagcca
    12121 gtcccttccc gcttcagtga caacgtcgag cacagctgcg caaggaacgc ccgtcgtggc
    12181 cagccacgat agccgcgctg cctcgtcctg cagttcattc agggcaccgg acaggtcggt
    12241 cttgacaaaa agaaccgggc gcccctgcgc tgacagccgg aacacggcgg catcagagca
    12301 gccgattgtc tgttgtgccc agtcatagcc gaatagcctc tccacccaag cggccggaga
    12361 acctgcgtgc aatccatctt gttcaatcct agggccggga ttctcctcca cgtcaccgca
    12421 tgttagaaga cttcctctgc cctctccgct gccagatctc tcgaggccct gtgggaggaa
    12481 gagaagaggt cagataactt cgtataatgt atgctatacg aagttatgcg gccgctggga
    12541 gaagggtaag agagctcaat cctcatctta catagaagga agtcagtaga taatatctta
    12601 catttaaaag tcaagaaata acagtctgac catactatct agaaatatgg aagttaacag
    12661 taaggaagtt aaaacagttg aaagtagttt cgtctagaaa gaaagacttg gggaagaaca
    12721 aagaaggcta attttcatta tgagctttat tgtaacagaa aagagaccac taactcagct
    12781 aggttttccc aattcctcaa cactttgggg cacagtgttt agtcagagct gtgatcactc
    12841 tctgagcaca tggattgtat cttaattttc atctcattga aaatatgtac catttgtcca
    12901 agtggaaact gggctgaagc atgactgtga ccaccaccaa agaaatggct agactcgcta
    12961 aggtctgctg gggtgacccg tgattgtgtt gtgcttgatc ttgttgagca tggctctttt
    13021 aagtcaccat acctgcccct gccaatgcac caaacaccaa cttaaaggga ctttcagctt
    13081 ttgtaagtca gtctcagcct tacttctgaa ccatttcttt gacttctttc atgactctga
    13141 tcctgtccca gttttaagcc tcccagccac cagtcatgct gttttgctct gctatttaca
    13201 ttcttctcta cctactttgg acttgatggt atgcctacta tattagttat ttattgctgt
    13261 gtaacaaacc actctgaaac atagtggctt acaaccacaa ccattttatt ttattatgaa
    13321 actgtgggtc agaatgtata ttggcccatt ggcatgattc tcctgctatt gaaaaaggaa
    13381 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
    13441 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
    13501 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
    13561 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
    13621 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
    13681 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
    13741 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
    13801 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
    13861 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
    13921 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
    13981 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
    14041 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
    14101 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
    14161 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
    14221 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
    14281 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
    14341 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
    14401 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
    14461 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
    14521 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
    14581 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcc
//
