LOCUS       sequence-236916-        8485 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Produces equimolar expression of yeast codon-optimized mVenus and 
            mTq2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8485)
  AUTHORS   Botman D, de Groot DH, Schmidt P, Goedhart J, Teusink B
  TITLE     In vivo characterisation of fluorescent proteins in budding yeast.
  JOURNAL   Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.1038/s41598-019-38913-z.
  PUBMED    30783202
REFERENCE   2  (bases 1 to 8485)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2019-02-19"; volume: "9(1):2234. doi"; pages: " 
            10.1038/s41598-019-38913-z"
COMMENT     Sequence Label: pDRF1-GW ymVenus-T2A-mTurquoise2
FEATURES             Location/Qualifiers
     source          1..8485
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    800..824
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             851..1564
                     /codon_start=1
                     /product="yellow fluorescent protein optimized for FRET 
                     with CyPet (Nguyen and Daugherty, 2005)"
                     /label=YPet
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             851..1564
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             1571..1624
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      1640..1649
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1646..1648,1649..1651,1652..2365)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1691..1712)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1952..1971)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2299..2320
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(2375..2399)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(2461..2480)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2462..2480)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      2628..2815
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2683..2707)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(2827..2844)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2827..2843)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2836..2858)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3279..4625
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(4736..5539)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(5540..5755)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(5780..5798)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5866..5970
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5971..6039,6040..6831)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6189..6208)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7002..7590
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7491..7510
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7744..7761
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7878..7899
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7914..7919,7920..7937,7938..7944)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7952..7968
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7957..7979
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7976..7992
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7976..7992
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(8022..8477)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8159..8180)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8371..8390
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 caagcttcct gaaacggaga aacataaaca ggcattgctg ggatcaccca tacatcactc
       61 tgttttgcct gaccttttcc ggtaatttga aaacaaaccc ggtctcgaag cggagatccg
      121 gcgataatta ccgcagaaat aaacccatac acgagacgta gaaccagccg cacatggccg
      181 gagaaactcc tgcgagaatt tcgtaaactc gcgcgcattg catctgtatt tcctaatgcg
      241 gcacttccag gcctcgatcg agaccgttta tccattgctt ttttgttgtc tttttccctc
      301 gttcacagaa agtctgaaga agctatagta gaactatgag ctttttttgt ttctgttttc
      361 cttttttttt tttttacctc tgtggaaatt gttactctca cactctttag ttcgtttgtt
      421 tgttttgttt attccaatta tgaccggtga cgaaacgtgg tcgatggtgg gtaccgctta
      481 tgctcccctc cattagtttc gattatataa aaaggccaaa tattgtatta ttttcaaatg
      541 tcctatcatt atcgtctaac atctaatttc tcttaaattt tttctctttc tttcctataa
      601 caccaatagt gaaaatcttt ttttcttcta tatctacaaa aacttttttt ttctatcaac
      661 ctcgttgata aattttttct ttaacaatcg ttaataatta attaattgga aaataaccat
      721 tttttctctc ttttatacac acattcaaaa gaaagaaaaa aaatataccc cagcctcgac
      781 tagtgaattc gcggccatca caagtttgta caaaaaagca ggctgctagc actagtaagc
      841 ttttaattaa atgtctaaag gtgaagaatt attcactggt gttgtcccaa ttttggttga
      901 attagatggt gatgttaatg gtcacaaatt ttctgtctcc ggtgaaggtg aaggtgatgc
      961 tacttacggt aaattgacct taaaattgat ttgtactact ggtaaattgc cagttccatg
     1021 gccaacctta gtcactactt taggttatgg tttgcaatgt tttgctagat acccagatca
     1081 tatgaaacaa catgactttt tcaagtctgc catgccagaa ggttatgttc aagaaagaac
     1141 tatttttttc aaagatgacg gtaactacaa gaccagagct gaagtcaagt ttgaaggtga
     1201 taccttagtt aatagaatcg aattaaaagg tattgatttt aaagaagatg gtaacatttt
     1261 aggtcacaaa ttggaataca actataactc tcacaatgtt tacatcactg ctgacaaaca
     1321 aaagaatggt atcaaagcta acttcaaaat tagacacaac attgaagatg gtggtgttca
     1381 attagctgac cattatcaac aaaatactcc aattggtgat ggtccagtct tgttaccaga
     1441 caaccattac ttatcctatc aatctaaatt atccaaagat ccaaacgaaa agagagacca
     1501 catggtcttg ttagaatttg ttactgctgc tggtattacc catggtatgg atgaattgta
     1561 caaatccgga gagggcagag gaagtcttct aacatgcggt gacgtggagg agaatcccgg
     1621 ccctggggat ccaccggtcg ccaccatggt gagcaagggc gaggagctgt tcaccggggt
     1681 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     1741 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
     1801 caagctgccc gtgccctggc ccaccctcgt gaccaccctg tcctggggcg tgcagtgctt
     1861 cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     1921 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     1981 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     2041 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tactttagcg acaacgtcta
     2101 tatcaccgcc gacaagcaga agaacggcat caaggccaac ttcaagatcc gccacaacat
     2161 cgaggacggc ggcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     2221 ccccgtgctg ctgcccgaca accactacct gagcacccag tccaagctga gcaaagaccc
     2281 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     2341 cggcatggac gagctgtaca agtaaagcgg ccgcacccag ctttcttgta caaagtggtg
     2401 tagttaattc atgatggccg ctgcaggtcg acctcgaggg ggggcccggt acccaattcg
     2461 ccctatagtg agtcgtatta cgcgcggatc cagctttgga cttcttcgcc agaggtttgg
     2521 tcaagtctcc aatcaaggtt gtcggcttgt ctaccttgcc agaaatttac gaaaagatgg
     2581 aaaagggtca aatcgttggt agatacgttg ttgacacttc taaataagcg aatttcttat
     2641 gatttatgat ttttattatt aaataagtta taaaaaaaat aagtgtatac aaattttaaa
     2701 gtgactctta ggttttaaaa cgaaaattct tattcttgag taactctttc ctgtaggtca
     2761 ggttgctttc tcaggtatag catgaggtcg ctcttattga ccacacctct accggcatgc
     2821 caattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     2881 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     2941 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt
     3001 tctccttacg catctgtgcg gtatttcaca ccgcataatc ggatcgtact tgttacccat
     3061 cattgaattt tgaacatccg aacctgggag ttttccctga aacagatagt atatttgaac
     3121 ctgtataata atatatagtc tagcgcttta cggaagacaa tgtatgtatt tcggttcctg
     3181 gagaaactat tgcatctatt gcataggtaa tcttgcacgt cgcatccccg gttcattttc
     3241 tgcgtttcca tcttgcactt caatagcata tctttgttaa cgaagcatct gtgcttcatt
     3301 ttgtagaaca aaaatgcaac gcgagagcgc taatttttca aacaaagaat ctgagctgca
     3361 tttttacaga acagaaatgc aacgcgaaag cgctatttta ccaacgaaga atctgtgctt
     3421 catttttgta aaacaaaaat gcaacgcgag agcgctaatt tttcaaacaa agaatctgag
     3481 ctgcattttt acagaacaga aatgcaacgc gagagcgcta ttttaccaac aaagaatcta
     3541 tacttctttt ttgttctaca aaaatgcatc ccgagagcgc tatttttcta acaaagcatc
     3601 ttagattact ttttttctcc tttgtgcgct ctataatgca gtctcttgat aactttttgc
     3661 actgtaggtc cgttaaggtt agaagaaggc tactttggtg tctattttct cttccataaa
     3721 aaaagcctga ctccacttcc cgcgtttact gattactagc gaagctgcgg gtgcattttt
     3781 tcaagataaa ggcatccccg attatattct ataccgatgt ggattgcgca tactttgtga
     3841 acagaaagtg atagcgttga tgattcttca ttggtcagaa aattatgaac ggtttcttct
     3901 attttgtctc tatatactac gtataggaaa tgtttacatt ttcgtattgt tttcgattca
     3961 ctctatgaat agttcttact acaatttttt tgtctaaaga gtaatactag agataaacat
     4021 aaaaaatgta gaggtcgagt ttagatgcaa gttcaaggag cgaaaggtgg atgggtaggt
     4081 tatataggga tatagcacag agatatatag caaagagata cttttgagca atgtttgtgg
     4141 aagcggtatt cgcaatattt tagtagctcg ttacagtccg gtgcgttttt ggttttttga
     4201 aagtgcgtct tcagagcgct tttggttttc aaaagcgctc tgaagttcct atactttcta
     4261 gctagagaat aggaacttcg gaataggaac ttcaaagcgt ttccgaaaac gagcgcttcc
     4321 gaaaatgcaa cgcgagctgc gcacatacag ctcactgttc acgtcgcacc tatatctgcg
     4381 tgttgcctgt atatatatat acatgagaag aacggcatag tgcgtgttta tgcttaaatg
     4441 cgtacttata tgcgtctatt tatgtaggat gaaaggtagt ctagtacctc ctgtgatatt
     4501 atcccattcc atgcggggta tcgtatgctt ccttcagcac taccctttag ctgttctata
     4561 tgctgccact cctcaattgg attagtctca tccttcaatg ctatcatttc ctttgatatt
     4621 ggatcgatcc gatgataagc tgtcaaacat gagaattggg taataactga tataattaaa
     4681 ttgaagctct aatttgtgag tttagtatac atgcatttac ttataataca gttttttagt
     4741 tttgctggcc gcatcttctc aaatatgctt cccagcctgc ttttctgtaa cgttcaccct
     4801 ctaccttagc atcccttccc tttgcaaata gtcctcttcc aacaataata atgtcagatc
     4861 ctgtagagac cacatcatcc acggttctat actgttgacc caatgcgtct cccttgtcat
     4921 ctaaacccac accgggtgtc ataatcaacc aatcgtaacc ttcatctctt ccacccatgt
     4981 ctctttgagc aataaagccg ataacaaaat ctttgtcgct cttcgcaatg tcaacagtac
     5041 ccttagtata ttctccagta gatagggagc ccttgcatga caattctgct aacatcaaaa
     5101 ggcctctagg ttcctttgtt acttcttctg ccgcctgctt caaaccgcta acaatacctg
     5161 ggcccaccac accgtgtgca ttcgtaatgt ctgcccattc tgctattctg tatacacccg
     5221 cagagtactg caatttgact gtattaccaa tgtcagcaaa ttttctgtct tcgaagagta
     5281 aaaaattgta cttggcggat aatgccttta gcggcttaac tgtgccctcc atggaaaaat
     5341 cagtcaagat atccacatgt gtttttagta aacaaatttt gggacctaat gcttcaacta
     5401 actccagtaa ttccttggtg gtacgaacat ccaatgaagc acacaagttt gtttgctttt
     5461 cgtgcatgat attaaatagc ttggcagcaa caggactagg atgagtagca gcacgttcct
     5521 tatatgtagc tttcgacatg atttatcttc gtttcctgca tgtttttgtt ctgtgcagtt
     5581 gggttaagaa tactgggcaa tttcatgttt cttcaacact acatatgcgt atatatacca
     5641 atctaagtct gtgctccttc cttcgttctt ccttctgttc ggagattacc gaatcaaaaa
     5701 aatttcaagg aaaccgaaat caaaaaaaag aataaaaaaa aaatgatgaa ttgaaaagct
     5761 aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     5821 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     5881 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     5941 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     6001 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     6061 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     6121 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     6181 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     6241 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     6301 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     6361 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     6421 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     6481 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     6541 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     6601 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     6661 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     6721 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     6781 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     6841 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     6901 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     6961 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     7021 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     7081 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     7141 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     7201 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     7261 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     7321 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     7381 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     7441 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     7501 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     7561 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     7621 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     7681 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     7741 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     7801 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     7861 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
     7921 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
     7981 aacagctatg accatgatta cgccaagctt accgcatcag gaaattgtaa gcgttaatat
     8041 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga
     8101 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc
     8161 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac
     8221 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc
     8281 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg
     8341 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag
     8401 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
     8461 gccgctacag ggcgcgtcca ttcgc
//
