LOCUS       sequence-236932-        4922 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast tagging vector to create a gene fusion with yeast optimized 
            mTq2 with CaUra3 selection. *An updated version of this plasmid is 
            available: #168057 pFA6a-link-ymTq2-URA3..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4922)
  AUTHORS   Botman D, de Groot DH, Schmidt P, Goedhart J, Teusink B
  TITLE     In vivo characterisation of fluorescent proteins in budding yeast.
  JOURNAL   Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.1038/s41598-019-38913-z.
  PUBMED    30783202
REFERENCE   2  (bases 1 to 4922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2019-02-19"; volume: "9(1):2234. doi"; pages: " 
            10.1038/s41598-019-38913-z"
COMMENT     Sequence Label: pFA6a-ymTurquoise2-CaURA3
FEATURES             Location/Qualifiers
     source          1..4922
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     65..84
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        165..183
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     165..182
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     terminator      1012..1199
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(1067..1091)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        1274..1617
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     terminator      2436..2633
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(2740..2759)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2741..2759)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2846..2863)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3017..3605)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3097..3116)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3776..4567,4568..4636))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4399..4418
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4637..4741)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4809..4827
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4865..4887)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac
       61 tatgcggcat cagagcagat tgtactgaga gtgcaccata tggacatatt gtcgttagaa
      121 cgcggctaca attaatacat aaccttatgt atcatacaca tacgatttag gtgacactat
      181 agaacgcggc cgccagctga agcttcgtac gctgcaggtc gacggatcgg tgacggtgct
      241 ggtttaatta aatggttagt aaaggtgaag aattgttcac tggtgtagtg ccaattttag
      301 tagaattaga tggtgacgtt aacggtcata agttctctgt aagcggtgaa ggtgaaggtg
      361 acgcaacata cggtaaatta actttgaaat tcatttgtac tacaggtaaa ttaccagttc
      421 catggccaac tttggttact actttgtctt ggggtgttca atgtttcgct agatacccag
      481 atcatatgaa gcaacatgat ttctttaagt ctgctatgcc agaaggttac gttcaagaaa
      541 gaactatctt cttcaaggat gatggtaact ataagactag agctgaagtt aagttcgaag
      601 gagatacttt agttaataga attgaattga agggtattga ttttaaggaa gatggtaata
      661 ttttgggtca taagttggaa tataattact tctctgataa cgtttacatt actgctgata
      721 agcaaaagaa cggtattaag gcgaacttca agattagaca taacattgaa gatggtggtg
      781 ttcaattagc tgatcattac caacaaaaca ctccaattgg tgacggtcca gttttgttgc
      841 cagataacca ttacttgtct actcaatcta agttgtctaa ggatccaaac gaaaagagag
      901 atcatatggt tttgttggaa ttcgttactg ctgctgggat cactttaggt atggatgaat
      961 tgtataaata agcggccgcc tcgaggtcga cggcgcgcca cttctaaata agcgaatttc
     1021 ttatgattta tgatttttat tattaaataa gttataaaaa aaataagtgt atacaaattt
     1081 taaagtgact cttaggtttt aaaacgaaaa ttcttattct tgagtaactc tttcctgtag
     1141 gtcaggttgc tttctcaggt atagtatgag gtcgctctta ttgaccacac ctctaccggc
     1201 agatccgcta gggataacag ggtaatatag atctgtttag cttgcctcgt ccccgccggg
     1261 tcacccggcc agcgacatgg aggcccagaa taccctcctt gacagtcttg acgtgcgcag
     1321 ctcaggggca tgatgtgact gtcgcccgta catttagccc atacatcccc atgtataatc
     1381 atttgcatcc atacattttg atggccgcac ggcgcgaagc aaaaattacg gctcctcgct
     1441 gcggacctgc gagcagggaa acgctcccct cacagacgcg ttgaattgtc cccacgccgc
     1501 gcccctgtag agaaatataa aaggttagga tttgccactg aggttcttct ttcatatact
     1561 tccttttaaa atcttgctag gatacagttc tcacatcaca tccgaacata aacaaccatg
     1621 acagtcaaca ctaagaccta tagtgagaga gcagaaactc atgcctcacc agtagcacaa
     1681 cgattatttc gattaatgga actgaagaaa accaatttat gtgcatcaat tgatgttgat
     1741 accactaagg aattccttga attaattgat aaattgggtc cttatgtatg cttaatcaag
     1801 actcatattg atataatcaa tgatttttcc tatgaatcca ctattgaacc attattagaa
     1861 ctttcacgta aacatcaatt tatgattttt gaagatagaa aatttgctga tattggtaat
     1921 accgtgaaga aacaatatat tggtggagtt tataaaatta gtagttgggc agatattact
     1981 aatgctcatg gtgtcactgg gaatggagta gttgaaggat taaaacaggg agctaaagaa
     2041 accaccacca accaagagcc aagagggtta ttgatgttag ctgaattatc atcagtggga
     2101 tcattagcat atggagaata ttctcaaaaa actgttgaaa ttgctaaatc cgataaggaa
     2161 tttgttattg gatttattgc ccaacgtgat atgggtggac aagaagaagg atttgattgg
     2221 cttattatga cacctggagt tggattagat gataaaggtg atggattagg acaacaatat
     2281 agaactgttg atgaagttgt tagcactgga actgatatta tcattgttgg tagaggattg
     2341 tttggtaaag gaagagatcc agatattgaa ggtaaaaggt atagagatgc tggttggaat
     2401 gcttatttga aaaagactgg ccaattataa tcagtactga caataaaaag attcttgttt
     2461 tcaagaactt gtcatttgta tagttttttt atattgtagt tgttctattt taatcaaatg
     2521 ttagcgtgat ttatattttt tttcgcctcg acatcatctg cccagatgcg aagttaagtg
     2581 cgcagaaagt aatatcatgc gtcaatcgta tgtgaatgct ggtcgctata ctgctgtcga
     2641 ttcgatacta acgccgccat ccagtgtcga aaacgagctc gaattcatcg atgatatcag
     2701 atccactagt ggcctatgcg gccgcggatc tgccggtctc cctatagtga gtcgtattaa
     2761 tttcgataag ccaggttaac ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     2821 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     2881 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     2941 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     3001 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     3061 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     3121 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     3181 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     3241 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     3301 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     3361 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     3421 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     3481 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     3541 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     3601 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     3661 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     3721 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     3781 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     3841 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     3901 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     3961 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     4021 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     4081 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     4141 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     4201 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     4261 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     4321 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     4381 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     4441 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     4501 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     4561 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     4621 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     4681 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     4741 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
     4801 cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg
     4861 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     4921 gg
//
