LOCUS       sequence-23694-e         904 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Ruby JG, Jan CH, Bartel DP
  TITLE     Intronic microRNA precursors that bypass Drosha processing.
  JOURNAL   Nature. 2007 Jul 5. 448(7149):83-6.
  PUBMED    17589500
REFERENCE   2  (bases 1 to 904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2007 Jul 5. 448(7149):83-6."
COMMENT     Sequence Label: CJ29 (let-7 perfect site)
FEATURES             Location/Qualifiers
     source          1..904
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             198..239
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             249..266
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(289..306)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    308..442
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(332..351)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     386..405
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(647..667)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 agttnctatc tgaatttgtc aagtaaaagg tcttcatttt tcgcaagaag atgcacctga
       61 tgaaatggga aaatatatca aatcgttcgt tgagcgagtt ctcaaaaatg aacaataatt
      121 ctaggagctc actatacaac ctactacctc aactagttgt tctagagcgg ccgctcgagt
      181 ctagagggcc cttcgaaggt aagcctatcc ctaaccctct cctcggtctc gattctacgc
      241 gtaccggtca tcatcaccat caccattgag tttaaacccg ctgatcagcc tcgactgtgc
      301 cttctaagat ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat
      361 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
      421 tataagctgc aataaacaag ttctagagtc ggtgggcctc gggggcgggt gcggggtcgg
      481 cggggccgcc ccgggtggct tcggtcggag ccatggggtc gtgcgctcct ttcggtcggg
      541 cgctgcgggt cgtggggcgg gcgtcaggca ccgggcttgc gggtcatgca ccaggtgcgc
      601 ggtccttcgg gcacctcgac gtcggcggtg acggtgaagc cgagccgctc gtagaagggg
      661 aggttgcggg gcgcggaggt ctccaggaag gcgggcaccc cggcgcgctc ggccgcctcc
      721 actccgggga gcacgacggc gctgcccaga cccttgccct ggtggtcggg cgagacgccg
      781 acggtggcca ggaaccacgc gggctccttg ggccggtgcg gcgccaggag gccttccatc
      841 tgttgctgcg cggccagccg gancgctcac tcggcagccg gcnttcggaa cgccntcact
      901 cggg
//
